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Preparation method of size-controllable giant liposome capable of being separated from substrate

A technology of giant liposomes and lipids, applied in the direction of microsphere preparation, chemical instruments and methods, microcapsule preparations, etc., can solve the problems of giant liposomes, expensive equipment, high cost, etc., and achieve easy large-scale industrialization The effect of controllable production and growth size and simple operation

Active Publication Date: 2021-10-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned methods all require higher process technology and expensive equipment, and the operation steps are complicated and costly.
At the same time, the edge of the microporous structure will have a strong adhesion to the membrane, making it difficult for the prepared giant liposomes to break away from the substrate and form a suspended state, which makes the application range of this method narrow

Method used

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  • Preparation method of size-controllable giant liposome capable of being separated from substrate
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  • Preparation method of size-controllable giant liposome capable of being separated from substrate

Examples

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Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 A method for preparing giant liposomes with controllable size and detachable substrate

[0032] The preparation process of giant liposomes is as follows: figure 1 Shown, concrete preparation method comprises the following steps:

[0033] (1) Cleaning of ITO glass substrate: Place the ITO glass substrate (a cube substrate with a thickness of 0.1 cm and a side length of 3 cm) in isopropanol, acetone, deionized water and absolute ethanol for 15 minutes for ultrasonic cleaning, and then use Dry with high-purity nitrogen for later use;

[0034] (2) Preparation of lipid molecule solution: Add 75 mg of POPC to a mixed solvent of 19 mL of chloroform and 1 mL of acetonitrile, then shake evenly until completely dissolved to obtain a lipid molecule solution with a concentration of 3 mg / mL;

[0035] (3) Preparation of lipid film: 400 μL lipid molecule solution was spin-coated on the ITO substrate to obtain a wet film sample. Dense dry lipid film covering the substrate,...

Embodiment 2

[0040] Example 2 A method for preparing giant liposomes with controllable size and detachable substrate

[0041] The preparation process of giant liposomes is as follows: figure 1 Shown, concrete preparation method comprises the following steps:

[0042] Step (1), (2), (3) are with embodiment 1;

[0043] (4) Preparation of island-shaped lipid film: fix the above-mentioned substrate covered with lipid film on the panel (working platform) of the high-precision electric displacement stage, set the movement parameter of the panel X-axis direction to 468 by the controller, and The step moving distance is 260 μm, and the lipid film is cut along the Y-axis direction with the microneedle for 10 cuts at each single step; then the substrate is rotated 90°, and the above cutting operation is repeated to obtain a 40 μm side length. Square island lipid membrane ( image 3 b).

[0044] (5) Preparation of giant liposomes: Paste a hollow insulating silica gel gasket with a thickness of 3m...

Embodiment 3

[0047] Example 3 A method for preparing giant liposomes with controllable size and detachable substrate

[0048] The preparation process of giant liposomes is as follows: figure 1 Shown, concrete preparation method comprises the following steps:

[0049] Step (1), (2), (3) are with embodiment 1;

[0050] (4) Preparation of island-shaped lipid film: fix the above-mentioned substrate covered with lipid film on the panel (working platform) of the high-precision electric displacement stage, set the movement parameter of the panel X-axis direction to 468 by the controller, and The step moving distance is 270 μm, and the lipid film is cut along the Y-axis direction with the microneedle for 10 cuts at each single step; then the substrate is rotated 90°, and the above cutting operation is repeated to obtain a 50 μm side length. Square island lipid membrane ( image 3 c).

[0051] (5) Preparation of giant liposomes: Paste a hollow insulating silica gel gasket with a thickness of 3m...

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Abstract

The invention belongs to the technical field of liposomes, particularly relates to a preparation method of a size-controllable giant liposome capable of being separated from a substrate, and aims to solve the problems that the size uniformity of the giant liposome prepared by the existing giant liposome preparation method is poor and is difficult to control. According to the invention, by means of a thin film microstructure processing device constructed by a high-precision displacement table and a cutting microneedle, a lipid membrane can be processed into square island-shaped lipid membrane structures with different sizes; and meanwhile, a single giant liposome with a specific size can be obtained on a single island-shaped lipid membrane in an external electric field stimulation mode, the size of the obtained giant liposome is in a range of 5-100 microns, and the growth size is controllable. Besides, the prepared giant lipidosome can be separated from the substrate and transferred into other solutions, so that many suspended giant lipidosome with good size uniformity can be obtained, can be transferred into an observation vessel to be controlled and loaded, and can be applied to more diverse research and wider application fields.

Description

technical field [0001] The invention belongs to the technical field of liposomes, and in particular relates to a method for preparing giant liposomes with controllable size and detachable substrate. Background technique [0002] Giant liposomes are single-layer closed spherical vesicles surrounded by bilayers formed by lipid molecules in aqueous solution through hydrophobic interactions, and their size is generally in the range of 1-100 μm. Because giant liposomes have the advantages of structure and composition similar to natural biological membranes, size and physical properties similar to real cells, and observation, implementation and operation under an optical microscope, they are widely used in molecular biology, cell biology, drug delivery, Chemical reactions and other fields have very broad application prospects. With the development of science and technology, various fields have put forward higher requirements for giant liposomes, including the controllability and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J13/04B01J19/08
CPCB01J13/02B01J13/04B01J19/087
Inventor 朱文鹏梁植华熊伟明邢未未郑跃
Owner SUN YAT SEN UNIV