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Metabonomics analysis method of Au-Pd/wtE. coli nano material

A technology of nanomaterials and analysis methods, applied in the field of metabolomics analysis of Au-Pd/wtE.coli nanomaterials, can solve the problem of little understanding of the interaction between Au-Pd core-shell nanoparticles and organisms, and the inherent interference of the interaction system The process and molecular mechanism are not clear, and the biological sample processing conditions, extraction conditions, and detection conditions in the metabolomics analysis method cannot be determined, so as to avoid abnormal metabolism and uneven sample collection, cost-effective, and simple design.

Active Publication Date: 2021-10-29
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

[0007] At present, there is still little understanding of the interaction between Au-Pd core-shell nanoparticles and organisms, and the internal intervention process and molecular mechanism of the interaction system are still unclear, so it is impossible to determine the metabolomics based on the target metabolites to be detected. Processing conditions, extraction conditions and detection conditions of biological samples in analytical methods

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  • Metabonomics analysis method of Au-Pd/wtE. coli nano material
  • Metabonomics analysis method of Au-Pd/wtE. coli nano material
  • Metabonomics analysis method of Au-Pd/wtE. coli nano material

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Embodiment 1

[0055] Example 1 Non-targeted and spatial metabolomics combined analysis method

[0056] The specific steps of this embodiment are as follows figure 1 shown, including:

[0057] 1. Biological preparation of Au-Pd / wtE.Coli nanomaterials:

[0058] (1) Reagent source

[0059] Escherichia coli wtE.coli (DH5[alpha]) strain was purchased from Novagen, Madison, Wisconsin. HUVECs were obtained from the American Tissue Culture Collection (ATCC). Tryptone, yeast powder, NaCl, HAuCl 4 ·3H 2 O, K 2 PdCl 4 、HCOONH 4 , 2,2,6,6-tetramethylpiperidine and lutidine-N-oxide were purchased from Sigma-Aldrich (Louis, MO, USA). Dulbecco's modified eagle medium (DMEM) and cell culture plates were purchased from Corning (NY, USA). Heat-inactivated fetal bovine serum (FBS), penicillin-streptomycin solution, phosphate-buffered saline (PBS) and trypsin were purchased from Gibco (ThermoFisher, USA). A 0.22 μm filter was purchased from Biosharp (Anhui, China). The reagents used were of analyti...

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Abstract

The invention relates to the field of nano material preparation and metabonomics analysis, in particular to a metabonomics analysis method of an Au-Pd / wtE. coli nano material. The metabonomics analysis method provided by the invention comprises the following steps: (1) performing DESI-MSI scanning on frozen sections of tissues or organs of an animal model to obtain DESI-MSI detection results of water-soluble metabolites and fat-soluble metabolites; wherein the animal model is an animal model which is fed with the Au-Pd / wtE.coli nano material; the DESI-MSI detection conditions comprise that in a positive mode, the voltage is 4.5-5kV; in the negative mode, the voltage is -4.5 to -5 kV; the spatial resolution is 50 to 100 [mu] m; the solvent spray is a 90-95% methanol aqueous solution; and (2) carrying out space metabonomics analysis on the DESI-MSI detection result. In addition, the metabonomics analysis method disclosed by the invention can also comprise the step of carrying out non-targeted metabonomics detection and analysis on the cells co-cultured by the Au-Pd / wtE. coli nano material. The invention provides a basis for evaluating the Au-Pd / wtE. coli synthesis technology and the biological response characteristic, and has a wide application prospect.

Description

technical field [0001] The invention relates to the fields of nanomaterial preparation and metabolomics analysis, in particular to a metabolomics analysis method for Au-Pd / wtE.coli nanomaterials. Background technique [0002] As a modern detection and analysis technology with typical characteristics and significant advantages, metabolomics has attracted great attention from researchers for its internal related principles and practical applications. This technology has shown significant advantages in the fields of revealing metabolic processes and life behaviors in the body. At the same time, research on further technological innovation and development based on modern metabolomics has been going on for many years with fruitful results. At present, metabolomics has successfully realized the detection and analysis of the structure and content of thousands of metabolite small molecules (<1500Da), revealing their inherent connection with organisms. [0003] Due to the advanta...

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Application Information

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IPC IPC(8): G01N27/64G01N30/02G01N30/72G01N30/86G01N30/88
CPCG01N27/64G01N30/02G01N30/72G01N30/8634G01N30/8696G01N30/88G01N2030/8813G01N2560/00G01N2570/00
Inventor 张定坤龚萌
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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