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Pine wood nematode inhibiting composition containing oligopeptide and validamycin

A technology of pine wood nematodes and Jinggangmycin, which is applied in the direction of nematicides, biocides, animal repellants, etc., can solve the problems of limited injection period, limited control drugs, and no longer exist

Active Publication Date: 2021-11-05
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the epidemic of pine wood nematode is not effectively controlled, not only will the landscape dominated by pine trees such as Huangshan pine cease to exist, but it will also cause serious damage to the ecological environment of our country.
However, there are currently very limited preventive medicines
For example, in terms of prevention and control, the main problems at present are: (1) Abamectin and emamectin are injected into the tree trunk, and the duration of the drug effect is short, only two years, and the injection period is also limited; (2) Others are under development. New drugs such as Japan's Green Guard cannot be widely used due to their high prices; (3) large areas of forests need spraying and other methods that are more convenient than trunk injection, but these existing agents are due to various reasons of safety and cost , difficult to do

Method used

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  • Pine wood nematode inhibiting composition containing oligopeptide and validamycin
  • Pine wood nematode inhibiting composition containing oligopeptide and validamycin
  • Pine wood nematode inhibiting composition containing oligopeptide and validamycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Separation and identification of oligopeptides in Sj18 extract

[0038] After the fermentation product of the fungus sj18 was filtered, the mycelium was collected and extracted in a water bath at 70°C. The water extract was adsorbed by macroporous resin, purified by activated carbon, and then purified by G-15 and G-10 dextran gel. The peaks were collected at 214nm by ultraviolet chromatography detector, and the functional identification was carried out to determine the main components. The peaks are collected in large quantities. For the method of functional testing, see the authorized patent "A method for high-throughput screening of pine wood nematode inhibitors" (patent number 2020112915539).

[0039] The collected peaks are further detected and collected by the sugar column. The detection and collection position is the main peak of the sugar column, and its retention time is 3.88min ( figure 1 . liquid chromatogram), and the purified fraction was denote...

Embodiment 2

[0047] Example 2: Polypeptide Synthesis and Toxicity Test

[0048] For the synthesis of oligopeptides, take EQCSCPQ as an example, see Figure 5

[0049] The synthetic oligopeptide was tested for toxicity, the effect is as follows Figure 6 shown. The results showed that the oligopeptide EQCSCPQ had a certain effect on killing nematodes. Considering that the spatial structure of the synthetic peptide might be different from that of the natural peptide, it was normal that the activity was not completely consistent.

Embodiment 3

[0050] Example 3: Nematicidal effect and compatibility of Sj18A

[0051] Take 0.1g Jinggangmycin soluble powder (20% effective content) and dissolve it in 20mL water, take 40μL tebuconazole suspending agent and add 20mL water to dissolve, and then follow "A high-throughput screening method for pine wood nematode inhibitors" ( In the method in Patent No. 2020112915539), 25 μL of Jinggangmycin aqueous solution and tebuconazole suspension were respectively added to 50 μL of nematode liquid. Test results such as Figure 7 As shown, although sj18A alone can kill nematodes ( Figure 7 in sj18A-1), but the use concentration is higher (0.25%), and it is difficult to kill nematodes at lower concentrations ( Figure 7 middle sj18A-2); however, the addition of Jinggangmycin and tebuconazole greatly improved its sensitivity, especially the addition of Jinggangmycin made the efficacy of low-concentration sj18A more than 3 times, and the combination of the two had a better effect it is g...

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Abstract

The invention relates to the field of biological pest control, and discloses a pine wood nematode inhibiting composition containing oligopeptide and validamycin. The oligopeptide in the composition is extracted from xylariaceae fungus sj18, and is composed of seven amino acids. The oligopeptide has the effect of inhibiting and killing pine wood nematodes, but the oligopeptide needs higher concentration when being independently used, so that the practicability is low. The validamycin alone has no nematode killing effect. When the two components are compounded for use, a remarkable synergistic effect is achieved, the use concentration is greatly reduced, a safe and efficient nematicide is obtained, the use cost and the pollution to the environment are greatly reduced, and the main action mechanism is to regulate associated flora of nematodes to kill pine wood nematodes.

Description

technical field [0001] The invention relates to the field of biological pest control, in particular to a composition for inhibiting pine wood nematode containing oligopeptide and Jinggangmycin. Background technique [0002] There are more than 80 species of pine trees in the world, mainly divided into masson pine, Chinese pine, white bark pine, Huangshan pine, black pine, Yunnan pine, golden pine, sylvestris pine, cedar and so on. Most of the above species are the main tree species for afforestation on barren hills in my country. However, in 1982, pine wood nematode was introduced from abroad in my country. Since then, the disease has spread rapidly. At present, the disease has been found in 588 county-level administrative regions in 18 provinces, with an occurrence area of ​​9.74 million mu. Pine wood nematode disease has become the number one forest disease and insect pest, and the damage is very serious. The total number of pine trees lost due to pine wood nematode disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N37/46A01N63/30A01P5/00
CPCA01N43/16A01N37/46A01N63/30
Inventor 朱汤军彭华正金群英叶华琳
Owner ZHEJIANG FORESTRY ACAD
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