Plant based functional materials
A plant-based, biological technology, applied in the fields of food science, silicon compounds, inorganic chemistry, etc., can solve the problems of plant-based protein unsuccessful biomaterials, low mechanical properties, low solubility, etc.
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Embodiment 1
[0169] Free-standing hydrogels were prepared according to the following procedure.
[0170] Glacial acetic acid was mixed with deionized water in different ratios (10% v / v, 30% v / v, 50% v / v, 70% v / v and 90% v / v). Soy protein isolate was added to the DI water / acetic acid solution at a final protein concentration of 100 mg / ml. A dispersion of insoluble protein is obtained. To solubilize the protein, the mixture was exposed to sonication for 30 minutes (high frequency power output = 70W, frequency = 20KHz, amplitude = 90%). During this process, the sample temperature was maintained at 85°C-90°C. After 30 minutes, a completely translucent liquid solution was obtained. The samples were cooled at room temperature for 5 min. During this process, the liquid sample turned into a clear and free-standing hydrogel that could be observed after the vial was inverted.
[0171] For different acetic acid / DI water ratios, the hydrogels prepared according to Example 1 were as figure 1 show...
Embodiment 2
[0193] Hydrogels were prepared using lactic acid as a co-solvent according to the following procedure.
[0194] Lactic acid was mixed with deionized water in different ratios (10% v / v, 30% v / v, 50% v / v, 70% v / v and 90% v / v). Soy protein isolate was added to the DI water / lactic acid solution at a final protein concentration of 100 mg / ml. A dispersion of insoluble protein is obtained. To solubilize the protein, the mixture was exposed to sonication for 30 min (high frequency power output = 70 W, frequency = 20 KHz, amplitude = 90%). During this process, the sample temperature was maintained at 85°C-90°C. After 30 min, a completely clear liquid solution was obtained. The samples were cooled at room temperature for 5 min.
[0195] gel electrophoresis
[0196] Different water:lactic acid ratios (from 0 to 90% v / v) were performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE 4-12% gels with MES buffer ) analysis of different hydrolyzed ...
Embodiment 3
[0199] Embodiment 3-microgel making
[0200]Microfluidic devices (droplet generators) were fabricated using standard soft lithography techniques using a negative master photoresist (SU83050). The continuous oil phase (2% 008-fluorosurfactant in Fluorinert FC-40) was loaded into a 2 ml tube while the dispersed liquid SPI phase (85 mg / ml SPI in 40% v / v acetic acid, kept at 85°C) into 1.5ml tubes and quickly placed on a heating block at 85°C. To prevent gelation of the SPI solution during transfer to the microfluidic device, a custom silicone heater (Holroyd Components) including 1 / 32" ID stainless steel tubing was used to maintain the connection between the SPI reservoir and the inlet in the microfluidic device. The temperature of the PTFE tubing. The silicon heater temperature is controlled by a custom-made temperature controller. The two solutions are pumped into a standard flow-focused droplet generator by a pressure-driven system (Elveflow OB1) to generate ~100 μm diamete...
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