Preparation method of test strip for rapidly detecting chlorothalonil residues in grains

A technology of chlorothalonil and test strips, applied in the field of detection

Pending Publication Date: 2021-11-09
HEILONGJIANG PONY TESTING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, time-resolved fluorescent microspheres are rarely reported as immunochromatographic detection methods for the detection of pesticide residues in grains as immunoprobes.

Method used

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  • Preparation method of test strip for rapidly detecting chlorothalonil residues in grains
  • Preparation method of test strip for rapidly detecting chlorothalonil residues in grains
  • Preparation method of test strip for rapidly detecting chlorothalonil residues in grains

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) The method for preparing fluorescent probes by coupling europium nanomaterials with monoclonal antibodies is as follows: take 800 μL of 0.2moL / L borate buffer solution in a 2mL centrifuge tube, add 200 μL of polystyrene fluorescent microsphere latex, and vortex Mix well; place the centrifuge tube in a digitally controlled high-power ultrasonic instrument for 12 minutes to disperse the latex material evenly in the solution; add 40 μL of 15 mg / mL EDC aqueous solution, vortex at room temperature for 10 minutes; then 14000r / min, 10℃ Centrifuge at high speed for 10 min under the conditions, discard the supernatant to remove excess EDC, redissolve the precipitate with 1mL borate buffer, repeat the sonication step; add a certain amount of antibody aqueous solution, vortex and mix, and transfer to a shaker shaker at room temperature After 12 hours, fully couple the antibody with the microsphere material; repeat the centrifugation step, discard the supernatant to remove the u...

Embodiment 2

[0023] (1) The method for preparing fluorescent probes by coupling europium nanomaterials with monoclonal antibodies is as follows: take 800 μL 0.2moL / L borate buffer solution in a 2 mL centrifuge tube, add 200 μL polystyrene fluorescent microsphere latex, and vortex to mix Uniformly; place the centrifuge tube in a digitally controlled high-power ultrasonic instrument for 10 minutes to disperse the latex material evenly in the solution; add 40 μL of 15 mg / mL EDC aqueous solution, vortex at room temperature for 15 minutes; then 14000r / min, 10℃ Centrifuge at high speed for 10min, discard the supernatant to remove excess EDC, redissolve the precipitate with 1mL boric acid buffer, repeat the sonication step; add a certain amount of antibody aqueous solution, vortex and mix, and transfer to a shaker shaker at room temperature for 12h , to fully couple the antibody to the microsphere material; repeat the centrifugation step, discard the supernatant to remove the uncoupled antibody, r...

Embodiment 3

[0029] (1) The method for preparing fluorescent probes by coupling europium nanomaterials with monoclonal antibodies is as follows: take 800 μL of 0.2moL / L borate buffer solution in a 2mL centrifuge tube, add 200 μL of polystyrene fluorescent microsphere latex, and vortex Mix well; place the centrifuge tube in a digitally controlled high-power ultrasonic instrument for 15 minutes to disperse the latex material evenly in the solution; add 40 μL of 15 mg / mL EDC aqueous solution, vortex at room temperature for 18 minutes; then 14000r / min, 10℃ Centrifuge at high speed for 10 min under the conditions, discard the supernatant to remove excess EDC, redissolve the precipitate with 1mL borate buffer, repeat the sonication step; add a certain amount of antibody aqueous solution, vortex and mix, and transfer to a shaker shaker at room temperature After 12 hours, fully couple the antibody with the microsphere material; repeat the centrifugation step, discard the supernatant to remove the u...

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Abstract

The invention discloses a preparation method of a test strip for rapidly detecting chlorothalonil residues in grains, and belongs to the technical field of detection. The method comprises the following steps: (1) preparing a fluorescent probe; (2) screening detection conditions of the test strip: the labeling concentration of a monoclonal antibody coupled with europium, the dosage of a fluorescent probe, the concentration of a detection antigen on a detection line, and the antigen spraying rate (0.4-0.8 [mu]L / cm) of the detection line; (3) preparing a time-resolved fluorescence immunochromatography test strip; (4) sample detection: (1) sample pretreatment; (2) screening extraction time; (3) screening a dilution ratio of a sample extracting solution; (4) detection analysis and qualitative analysis: under an ultraviolet lamp, judging a qualitative result through naked eyes; and quantitative analysis: within 30 seconds after the reaction is finished, reading a result by adopting a fluorescence reading instrument to obtain the content of chlorothalonil in the detected grains. The test strip detection method for chlorothalonil residues, established by the invention, has the advantages of good accuracy, precision and reproducibility, simplicity and rapidness in operation and visual detection.

Description

technical field [0001] The invention belongs to the technical field of detection, and mainly relates to a preparation method of a test strip for rapidly detecting chlorothalonil residues in grains. Background technique [0002] my country is a big producer and user of pesticides. According to statistics, my country can produce 3.82 million tons of pesticides every year, accounting for about one-tenth of the world's total pesticide production; the amount of pesticides used is also much higher than the world average level, and the total annual use of pesticides is as high as 1.3 million tons. There are many kinds of pesticides, which can be divided into organic pesticides and inorganic pesticides according to their chemical structure, among which organic pesticides can be subdivided into organochlorines, organophosphorus, pyrethroids and carbamates, etc. The most widely used pesticides in the agricultural field are also the types of pesticides with the most serious residues a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N30/02G01N30/72G01N33/53G01N33/533G01N33/558G01N33/577G01N33/58
CPCG01N21/6428G01N21/643G01N30/02G01N30/72G01N33/577G01N33/558G01N33/5308G01N33/582G01N33/585G01N33/533
Inventor 王中江郭增旺赫志强韩丽杰王卓
Owner HEILONGJIANG PONY TESTING TECH CO LTD
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