Coprinus comatus mycelium polysaccharide as well as preparation method and application thereof

A technology of mycelium polysaccharides and Coprinus comatus, applied in biochemical equipment and methods, methods based on microorganisms, pharmaceutical formulations, etc., can solve the problems that affect the wide application of Coprinus comatus polysaccharides, vulnerability to climate influence, long cultivation cycle and cost and other problems, to achieve good antioxidant activity, easy to control growth, and increase yield

Inactive Publication Date: 2021-11-16
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the sporocarps of Coprinus comatus mainly obtained by wild picking or artificial cultivation, and then the polysaccharides of Coprinus comatus are extracted, which requires long cultivation period and cost for picking and cultivating, and is easily affected by climate. , thus affecting the wide application of Coprinus comatus polysaccharide in medicine

Method used

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  • Coprinus comatus mycelium polysaccharide as well as preparation method and application thereof
  • Coprinus comatus mycelium polysaccharide as well as preparation method and application thereof
  • Coprinus comatus mycelium polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Inoculate Coprinus comatus bacteria into a plate containing PDA, activate it in a constant temperature incubator at 28°C, and wait until the mycelia cover the plate. Take 1cm with a sterile punch 2 Mycelium was inoculated into 200mL liquid medium (potato 20.0g, glucose 2.0g, MgSO 4 ·7H 2 0 1.5g, KH2 PO 4 1.5 g, 1000 mL of water, the pH of which is 7), static culture at 26.5° C. for 1 day, and then shake on a rotary shaker at 180 rpm for 7 days to obtain a liquid culture solution. The liquid culture solution is dried at 80° C., crushed and passed through a 200-mesh sieve to obtain Coprinus comatus mycelium powder.

[0041] (2) Single factor experiment: using water as the extraction solvent, the extraction time (1, 2, 3, 4, 5h), the extraction temperature (50, 60, 70, 80, 90, 100°C), the ratio of solid to liquid ( 10:1, 20:1, 30:1, 40:1, 50:1mL / mg) as variables, taking the yield of polysaccharide (IPS) as a reference, the hydrothermal treatment of Coprinus comatu...

Embodiment 2

[0067] (1) obtain Coprinus comatus mycelium by fermenting and cultivating according to the optimal culture condition of embodiment 1, get Coprinus comatus mycelium drying and pulverizing, pour it into 200 mesh sieves subsequently and obtain Coprinus comatus mycelium powder;

[0068] (2) According to the optimized experimental conditions in Example 1, under actual operation, the optimal extraction process was adjusted to a liquid-to-solid ratio of 21:1 and leaching in a hot water bath at 81°C for 2 hours to obtain an extract;

[0069] (3) After filtering the extract, centrifuge and collect the supernatant: filter the extract with a sieve, and centrifuge the obtained liquid mixture at 3000r / min for 15min, collect the supernatant and carry out concentrated dialysis;

[0070] (4) Add absolute ethanol (final concentration: 75%) to the collected supernatant, then let it stand overnight at 4°C, discard the supernatant and collect the precipitate;

[0071] (5) Freeze-drying: place the...

Embodiment 3

[0094] Example 3 Research on Antioxidant Activity of Coprinus comatus Mycelia Polysaccharides

[0095] Antioxidative ability of polysaccharides and 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) free radical, 1,1-diphenyl-2-trinitrate It is related to the scavenging and reducing ability of diphenylhydrazine (DPPH) free radicals, hydroxyl free radicals, and superoxide anion free radicals. This embodiment measures the scavenging rates of these five free radicals to determine their antioxidant capacity.

[0096] (1) ABTS free radical scavenging activity:

[0097] ① ABTS stock solution (7.4mmol / L) and potassium persulfate (K 2 S 2 o 8 ) stock solution (2.6mmol / L) was mixed at a volume ratio of 1:1, stored in the dark for 16 hours, diluted 40-50 times with pH 7.4 phosphate buffer (0.01mol / L), and then measured at a wavelength of 734nm. Finally, the absorbance of the solution is stabilized at A 734nm =0.700±0.01. The sample concentration is 0.5, 1....

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Abstract

The invention relates to the technical field of polysaccharide extraction, in particular to coprinus comatus mycelium polysaccharide and a preparation method and application thereof. The monosaccharide of the coprinus comatus mycelium polysaccharide comprises fucoidin, glucose, galactose, glucuronic acid, mannose, rhamnose and xylose; the coprinus comatus mycelium polysaccharide has an alpha-configuration glucosidic bond. The preparation method comprises the following steps: performing liquid fermentation on a coprinus comatus strain to obtain fermentation liquor, drying and crushing to obtain coprinus comatus mycelium; and carrying out hydrothermal extraction on the coprinus comatus mycelium to obtain an extracting solution, centrifuging, collecting supernate, and carrying out alcohol precipitation and drying to obtain the coprinus comatus mycelium polysaccharide. The coprinus comatus mycelium polysaccharide disclosed by the invention has relatively strong antioxidant activity; according to the present invention, the mouse spleen lymphocyte proliferation can be promoted, the macrophage RAW264.7 growth can be activated, the macrophage RAW264.7 can be promoted to produce NO, and the secretion of immune-related cell factors such as IL-1beta, IL-6, IL-10, TNF-alpha and the like can be promoted;.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a Coprinus comatus mycelium polysaccharide, a preparation method and application thereof. Background technique [0002] Coprinus comatus, also known as thorny mushroom, is named for its shape like a chicken leg. It is a rare fungus with commercial potential that has been artificially developed in recent years, and is known as "the new star in the fungus". Coprinus comatus is rich in nutrition, delicious in taste, excellent in taste, and has high nutritional value. It has been proven to contain rich biologically active substances, excellent biological activity and medicinal value. In recent years, the health value of Coprinus comatus has gradually aroused people's awareness, and it has been identified by the Food and Agriculture Organization of the United Nations and the World Health Organization as one of the 16 rare edible fungi with three functions of "natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A61K31/715A61K36/07A61P37/04A61P39/06C12P19/04C12R1/645
CPCC08B37/0003C08B37/006C12P19/04A61K31/715A61K36/07A61P39/06A61P37/04A23L33/125A23V2002/00A23V2200/324A23V2200/30A23V2250/51
Inventor 宋琳鞠豪于志洋高羿牛蕴泽陈冉
Owner QINGDAO UNIV OF SCI & TECH
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