Azo dye degrading bacterium and application thereof
A technology of azo dyes and degrading bacteria, which is applied in the field of microorganisms, can solve the problems of difficult azo dye wastewater treatment, high salinity of printing and dyeing wastewater, and difficulty for microorganisms to adapt to high-salt environment, etc., achieving remarkable degradation effect and good decolorization effect
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Embodiment 1
[0026] Isolation of strain Marinobacterium S1: 1) Take 10 ml of activated sludge sample into the culture medium with 5% salinity, add 50 mg / L Acid Scarlet GR, after enrichment until there is obvious decolorization phenomenon, then in 5% salinity Streak continuously in the culture medium, culture at 30 °C until decolorization, pick a single colony for further purification and inoculate in a 5% salinity medium containing 100 mg / L Acid Scarlet GR, and culture it statically at 30 °C.
[0027] The culture medium of described 5% salinity consists of: Na 2 SO 4 0.5g; NH 4 Cl 0.3 g; CaCl 2 0.1 g; KH 2 PO 4 0.2g; KCl 0.5g; MgCl 2 ·6H 2 O 6 g; NaCl 40.0 g, yeast powder 3.0 g, pH 7.0, 1×10 5 Pa sterilized for 30min vested.
[0028] The strain Marinobacterium S1 is Gram-negative, facultative anaerobic bacteria, and its color is milky white.
[0029] Bacterial genomic DNA was extracted and amplified by PCR using thin 16SrRNA universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3', SEQ...
Embodiment 2
[0031] Research on the decolorization of Acid Scarlet GR by Marinobacterium sp.S1: the strain Marinobacterium sp.S1 screened in Example 1 was inoculated in a medium with 5% salinity, and cultured statically at 30 °C for 12 h. Inoculate Marinobacterium sp.S1 at 10% (V / V) in a 5% salinity medium containing 100 mg / L Acid Scarlet GR, culture at 30 °C, and measure the absorbance of the culture solution every 2 h Calculate the decolorization rate. Repeat 3 times. Taking time as the abscissa and the decolorization rate as the ordinate, draw a graph, and the results are shown in Figure 2.
Embodiment 3
[0033] The effect of salinity on the degradation of Acid Scarlet GR by Marinobacterium sp.S1: In order to study the effect of salinity on the degradation of Acid Scarlet GR by Marinobacterium sp. %, 7% and 9% culture medium, the pH of the culture medium is 7.5, and the concentration of Acid Scarlet GR is 100 mg / L. In a constant temperature incubator at 30 ℃, the samples are taken at regular intervals, and the remaining The concentration of Acid Scarlet GR, with time as the abscissa, and the degradation rate as the ordinate, the results are as follows image 3 It was shown that at a salinity of 1% to 7%, the degradation rate of acid scarlet GR by the flora can reach more than 88%.
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