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Application of PAIP1 autoantibody in auxiliary diagnosis of esophageal squamous carcinoma

A technology for auxiliary diagnosis and esophageal squamous cell carcinoma, applied in the field of medicine and biology, it can solve the problems of high markers, lack of specificity and sensitivity, and achieve the effect of reducing mortality and avoiding invasive diagnosis.

Pending Publication Date: 2021-11-19
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, up to now, in esophageal squamous cell carcinoma, there is still no marker with strong specificity and high sensitivity

Method used

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  • Application of PAIP1 autoantibody in auxiliary diagnosis of esophageal squamous carcinoma
  • Application of PAIP1 autoantibody in auxiliary diagnosis of esophageal squamous carcinoma
  • Application of PAIP1 autoantibody in auxiliary diagnosis of esophageal squamous carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Using human proteome chips to screen markers for the diagnosis of esophageal squamous cell carcinoma

[0035] 1. Experimental sample:

[0036]The sera of 30 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 24 normal subjects (normal control group) were collected from the specimen bank of the Henan Provincial Key Laboratory of Cancer Epidemiology; among them, the serum of 30 patients with esophageal squamous cell carcinoma came from Patients with esophageal squamous cell carcinoma confirmed by pathology without any treatment; 24 normal human serums were obtained from healthy subjects, and the inclusion criteria for healthy subjects were: no cardiovascular, respiratory, liver, kidney, gastrointestinal tract , endocrine, blood, mental, or nervous system diseases and the history of the above diseases, no acute or chronic diseases, no autoimmune diseases, no evidence of any tumor-related; moreover, 30 patients with es...

Embodiment 2

[0055] Example 2: ELISA detection of serum expression levels of autoantibodies against tumor-associated antigen PAIP1

[0056] Enzyme linked immunosorbent assay (ELISA) was used to further detect the expression level of anti-PAIP1 autoantibodies in the plasma of large sample population.

[0057] 1. Experimental sample:

[0058] The samples of 160 patients with primary esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 229 normal controls (normal control group) included in this study were all obtained from the specimen bank of the Key Laboratory of Cancer Epidemiology in Henan Province; among them, 160 cases The serum of patients with esophageal squamous cell carcinoma was obtained from patients with esophageal squamous cell carcinoma confirmed by pathology without any treatment; the serum of 160 normal people was obtained from healthy subjects, and the inclusion criteria for healthy subjects were: no cardiovascular, respiratory, Liver, kidney, g...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a biomarker for auxiliary diagnosis of esophageal squamous carcinoma and a detection kit. The biomarker for auxiliary diagnosis of esophageal squamous carcinoma provided by the invention is an autoantibody of an anti-tumor associated antigen PAIP1, the expression level of the marker in serum of a patient with esophageal squamous carcinoma is higher than that of a normal person, and the expression level has significant difference. The invention also provides a kit for auxiliary diagnosis of esophageal squamous carcinoma, the kit contains a reagent for detecting the marker, and the reagent is used for detecting the biomarker in a sample through enzyme-linked immunosorbent assay, a protein chip, immunoblotting or microfluidic immunoassay. By detecting the expression level of the anti-tumor associated antigen PAIP1 in human serum, esophageal squamous carcinoma patients and normal people can be effectively distinguished, and the kit can be used for auxiliary diagnosis of esophageal squamous carcinoma.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses the application of PAIP1 autoantibody in auxiliary diagnosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer (EC) is one of the common digestive tract cancers, which seriously threatens human health all over the world. Global cancer epidemiological statistics show that in 2020, the incidence and mortality of esophageal cancer rank the 8th and 6th among malignant tumors, respectively. China, in particular, is a country with a high incidence of esophageal cancer in the world, and the incidence and mortality of esophageal cancer ranks sixth and fourth among malignant tumors in China. Esophageal squamous cell carcinoma accounts for more than 90% of all esophageal cancers in China, and is the most common esophageal cancer in China. [0003] Esophageal squamous cell carcinoma has an insidious onset and a poor prognosis. I...

Claims

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Application Information

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IPC IPC(8): G01N33/564G01N33/574
CPCG01N33/564G01N33/57407G01N33/57488G01N2333/47
Inventor 代丽萍杨倩李科明仵金玉王鹏叶华史健翔
Owner ZHENGZHOU UNIV
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