Excrement preserving fluid, preparation method and application thereof

Abstract

An excrement preserving fluid provided by the invention comprises trihydroxymethyl aminomethane, ethylenediamine tetraacetic acid, sodium chloride, polyethylene glycol octylphenol ether, ethanol and the balance of deionized water, and the excrement preserving fluid provided by the invention has different action reagents, can effectively protect human body exfoliated cells in excrement, prevents the exfoliated cells from being further degraded, the next step of detection aiming at the cast-off cells can be conveniently carried out; and exfoliated cells in the excrement sample can be effectively preserved, the sample can be preserved at normal temperature for a long time, cell damage, nucleic acid degradation and microbial activity in the sample can be effectively inhibited, and subsequent detection is not influenced. In addition, the invention further provides a preparation method and application of the excrement preserving fluid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a feces preservation solution, a preparation method and an application thereof. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive system, and its morbidity and mortality rank among the top in China. In my country, about 380,000 new patients with colorectal cancer are diagnosed every year, and the number of deaths due to colorectal cancer is as high as 180,000. Since the early symptoms of colorectal cancer are not obvious, 20% of patients with colorectal cancer have metastasized when they are first diagnosed, and the 5-year survival rate of colorectal cancer patients without metastasis is as high as 90% after active treatment. rate down to 10%. This shows that early diagnosis of colorectal cancer is particularly important. [0003] At the current stage, the application of high-throughput sequencing technology has further promot...

AI Technical Summary

Problems solved by technology

[0004] However, feces contain a large number of complex components, such as protease, DNase and other nucleic acid degrading enzymes, which make the cells and nucleic acids in the stool sample vulnerable to damage and degradation.

At present, the commonly used preservation method is to place the sample at -20°C or lower after collection to avoid the degradation of exfoliated cells or other biochemical reactions. This leads to further loss of exfoliated cells, and extraction failure affects subsequent detection

The current methods of fecal sample storage have shortcomings such as short storage time, low sample availability after storage, and harsh storage conditions.

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