Excrement preserving fluid, preparation method and application thereof

A technology of preservation solution and feces, applied in the biological field, can solve the problems of loss of exfoliated cells, short storage time, detection failure of extraction, etc., and achieve the effect of inhibiting cell damage and preventing degradation

Pending Publication Date: 2021-12-03
SUZHOU ZHONGKE ADVANCED TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, feces contain a large number of complex components, such as protease, DNase and other nucleic acid degrading enzymes, which make the cells and nucleic acids in the stool sample vulnerable to damage and degradation.
At present, the commonly used preservation method is to place the sample at -20°C or lower after collection to avoid the degradation of exfoliated cells or other biochemical reactions. This leads to further loss of exfoliated cells, and extraction failure affects subsequent detection
The current methods of fecal sample storage have shortcomings such as short storage time, low sample availability after storage, and harsh storage conditions.

Method used

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  • Excrement preserving fluid, preparation method and application thereof
  • Excrement preserving fluid, preparation method and application thereof
  • Excrement preserving fluid, preparation method and application thereof

Examples

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preparation example Construction

[0038] The present application also provides a method for preparing said feces preservation solution, comprising the following steps:

[0039] Dissolving trishydroxymethylaminomethane, ethylenediaminetetraacetic acid, sodium chloride, polyethylene glycol octylphenyl ether and ethanol in deionized water; filtering the above solution to obtain the feces preservation solution.

[0040] The feces preservation solution is used for preserving human feces.

[0041] The feces preservation solution provided by this application has different functional reagents, which can effectively protect the exfoliated cells in the feces and prevent further degradation of the exfoliated cells, so as to facilitate the next step detection of the exfoliated cells; and can effectively preserve the exfoliated cells in the feces samples Exfoliated cells can store samples at room temperature for a long time, effectively inhibiting cell damage, nucleic acid degradation and microbial activity in samples, wit...

Embodiment 1

[0044] This embodiment provides a kind of preservation solution and application thereof of exfoliated cells in human feces, including: 0.2mol / L Tris, 0.025mol / L EDTA, 0.14mol / L NaCl, 1.5% Triton X- 100, 5% ethanol and deionized water.

[0045] The preparation method of exfoliated cell preservation liquid in described feces, comprises the following steps:

[0046] 1) Dissolve 200 mol parts of Tris, 25 mol parts of EDTA, 140 mol parts of NaCl, 15 parts by volume of Triton X-100 and 50 parts by volume of ethanol in deionized water, and set the volume to 1 L. The concentration of Tris in the feces preservation solution after constant volume is 0.2mol / L, the concentration of EDTA is 0.025mol / L, the concentration of NaCl is 0.14mol / L, the concentration of Triton X-100 is 1.5%, the concentration of ethanol 5%. Wherein, the ratio of molar parts and volume parts is mmol:mL.

[0047] 2) Filter the feces preservation solution obtained in step 1) with a disposable membrane filter devic...

Embodiment 2

[0050] The application provides a preservation solution of exfoliated cells in human feces and its application, including: 0.3mol / L Tris, 0.03mol / L EDTA, 0.14mol / L NaCl, 1.5% Triton X-100 , 10% ethanol and deionized water.

[0051] The preparation method of exfoliated cell preservation liquid in described feces, comprises the following steps:

[0052] 1) Dissolve 300 mol parts of Tris, 30 mol parts of EDTA, 140 mol parts of NaCl, 15 parts by volume of Triton X-100 and 100 parts by volume of ethanol in deionized water, and set the volume to 1 L. The concentration of Tris in the feces preservation solution after constant volume is 0.3mol / L, the concentration of EDTA is 0.03mol / L, the concentration of NaCl is 0.14mol / L, the concentration of Triton X-100 is 1.5%, the concentration of ethanol 10%. Wherein, the ratio of molar parts and volume parts is mmol:mL.

[0053] 3) Use a disposable filter membrane device to filter the feces preservation solution obtained in step 1), and stor...

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PUM

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Abstract

An excrement preserving fluid provided by the invention comprises trihydroxymethyl aminomethane, ethylenediamine tetraacetic acid, sodium chloride, polyethylene glycol octylphenol ether, ethanol and the balance of deionized water, and the excrement preserving fluid provided by the invention has different action reagents, can effectively protect human body exfoliated cells in excrement, prevents the exfoliated cells from being further degraded, the next step of detection aiming at the cast-off cells can be conveniently carried out; and exfoliated cells in the excrement sample can be effectively preserved, the sample can be preserved at normal temperature for a long time, cell damage, nucleic acid degradation and microbial activity in the sample can be effectively inhibited, and subsequent detection is not influenced. In addition, the invention further provides a preparation method and application of the excrement preserving fluid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a feces preservation solution, a preparation method and an application thereof. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive system, and its morbidity and mortality rank among the top in China. In my country, about 380,000 new patients with colorectal cancer are diagnosed every year, and the number of deaths due to colorectal cancer is as high as 180,000. Since the early symptoms of colorectal cancer are not obvious, 20% of patients with colorectal cancer have metastasized when they are first diagnosed, and the 5-year survival rate of colorectal cancer patients without metastasis is as high as 90% after active treatment. rate down to 10%. This shows that early diagnosis of colorectal cancer is particularly important. [0003] At the current stage, the application of high-throughput sequencing technology has further promot...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12Q1/24
CPCA01N1/021C12Q1/24
Inventor 王文婧张炳为
Owner SUZHOU ZHONGKE ADVANCED TECH RES INST CO LTD
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