Preservative for preventing and treating pear anthracnose and application thereof

A technology of antistaling agent and anthracnose, which is applied in the field of antistaling agent for the prevention and treatment of pear anthracnose, which can solve the problems of toxicity, residue, and human hazards, and achieve the effects of delaying drug resistance, reducing usage, and significant economic and social benefits

Active Publication Date: 2021-12-03
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical preservatives can play an antiseptic and fresh-keeping role, most of them have varying degrees of toxicity and residues, which are potentially harmful to the human body

Method used

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  • Preservative for preventing and treating pear anthracnose and application thereof
  • Preservative for preventing and treating pear anthracnose and application thereof
  • Preservative for preventing and treating pear anthracnose and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The virulence of embodiment 1 bamboo vinegar liquid and bactericide to test phytopathogenic bacteria

[0024] Crop pathogens tested: pear anthracnose

[0025] Determination method: The mycelium growth rate method was used to determine the toxicity of bamboo vinegar and fungicides to the tested plant pathogenic bacteria.

[0026] Dilute the tested bamboo vinegar solution to an appropriate concentration with sterile water. Take 1 mL of diluted bamboo vinegar solution, add it to 24 mL of PDA medium and mix evenly, pour it into a culture dish to make a culture plate containing bamboo vinegar. The PDA medium plate added with the same amount of sterile water was used as blank control (CK).

[0027] The fungicide to be tested was dissolved in acetonitrile and diluted to an appropriate concentration. Take 1 mL of the fungicide solution, mix it with 24 mL of PDA medium, and pour it into a petri dish to make different concentrations of drug-containing medium. The PDA medium pl...

Embodiment 2

[0031] Embodiment 2 compound combination joint action test

[0032] According to the EC50 of each single agent and bamboo vinegar solution, various proportioning combinations were designed through orthogonal experiments, and each proportioning combination was diluted to an appropriate concentration with sterile water. Take 1 mL of diluted bamboo vinegar solution, add it to 24 mL of PDA medium and mix evenly, pour it into a culture dish to make a culture plate containing bamboo vinegar. The PDA medium plate added with the same amount of sterile water was used as blank control (CK).

[0033] Under a sterile environment, use a puncher to make a bacterium cake with a diameter of 5.0 mm for the test bacteria cultured on the solid PDA medium. Inoculate the bacteria cake of the tested pathogenic bacteria (mycelia face down). Invert the inoculated PDA medium plate and place it in an incubator at 28°C for cultivation. Cultivate until the CK hyphae grow to about 85% of the petri dish...

Embodiment 3

[0047] Embodiment 3 living body antibacterial activity assay

[0048] Test method: in the embodiment, the formula and different medicaments are used for fruit dipping treatment, and each fruit is pricked (3mm deep) after drying, and each fruit is pricked 3 times. Paste 5mm bacteria cake in each hole, and each group handles 6 pears. After culturing at room temperature for 6 days, the diameter of the lesion was measured to verify the control effect of the agent on anthracnose.

[0049] Test drug:

[0050] Table 5 Composition of compound ingredients (1L) and prevention and control objects

[0051] Composite group name bamboo vinegar thiophanate-methyl Tebuconazole pyraclostrobin Auxiliary dosage form Control object (Compound 1) pyraclostrobin · tebuconazole · bamboo vinegar 745.7ml / 11.4g 17g 225.9g water emulsion anthrax (Compound 3) pyraclostrobin·thiophanate-methyl·bamboo vinegar 844.6ml 43.9g / 43.9g 67.6g Suspending agent ...

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Abstract

The invention provides a preservative for preventing and treating pear anthracnose, the active components in the preservative comprise a medicament A, a medicament B and bamboo vinegar, and the medicament A and the medicament B are a combination of pyraclostrobin and tebuconazole or a combination of pyraclostrobin and thiophanate-methyl. The method can effectively control the harm of anthracnose to the storage period of pears, effectively reduce the dosage of medicaments, delay the drug resistance of anthracnose to medicaments, improve the preservation effect and reduce the storage loss, and has remarkable economic and social benefits.

Description

technical field [0001] The invention belongs to the field of plant preservatives, and in particular relates to a preservative for preventing and treating pear anthracnose and an application thereof. Background technique [0002] Pear anthracnose, also known as bitter rot, is a disease that occurs on pears caused by the infestation of C. The pathogen is Colletrichum gloeosporioides Penz. The pathogen survives the winter as mycelium on stiff fruit or diseased branches, and infects pear trees in the second year when conditions are suitable. After picking, the disease gradually develops as the vitality of pears decreases, and often continues to expand during storage and even causes a large number of fruits to rot, causing certain economic losses. Pear anthracnose is common and occurs in pear producing areas all over the world. [0003] At present, pear storage and preservation methods include chemical preservatives, natural preservatives, biological preservatives, and control...

Claims

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Application Information

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IPC IPC(8): A23B7/154A01N47/24A01N43/653A01N47/34A01N61/00A01P3/00
CPCA23B7/154A01N47/24A01N43/653A01N47/34A01N61/00A23V2002/00A23V2200/222A23V2200/242A23V2250/5086A23V2250/51088A23V2250/5022
Inventor 吴祥为唐勇峰胡奎奎李晓萌
Owner ANHUI AGRICULTURAL UNIVERSITY
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