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Collagen composite material as well as preparation method and application thereof

A technology of collagen and composite materials, which is applied in prosthetics, medical science, etc., can solve the problems of the limitation of the degree of regulation of the degradation rate of the amount of chemical cross-linking agent and the impact on the biocompatibility of the collagen matrix, and achieve the controllable degradation rate, The effect of faster degradation rate and slower degradation rate

Pending Publication Date: 2021-12-03
江苏集萃新型药物制剂技术研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of chemical cross-linking agents can effectively adjust the degradation time of the collagen matrix, the chemical cross-linking agents used are cytotoxic and will affect the biocompatibility of the collagen matrix. Therefore, the amount of chemical cross-linking agents and the degradation rate control There are certain limits

Method used

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  • Collagen composite material as well as preparation method and application thereof
  • Collagen composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Preparation of collagen solution

[0057] Adjust the temperature of the cooling water bath of the reaction kettle to 5°C, add collagen and 0.08M acetic acid solution, stir until dissolved, and prepare the collagen concentration in the solution to be 10mg / ml.

[0058] (2) Preparation of polymer solution

[0059] Add high molecular polymer (polyethylene oxide, molecular weight 220kDa) and 0.08M acetic acid solution into a sealed glass bottle, put it in a 50°C water bath, stir until dissolved and cool to room temperature, the final concentration of the prepared solution is 10mg / ml .

[0060] (3) mixed solution

[0061] The above two solutions were stirred and mixed in a reaction kettle at 5°C, and the final mass ratio of collagen to polymer was 4:1. Then vacuum degas below 20 Pa for 30 minutes.

[0062] (4) Freeze-drying film production

[0063] Put the above-mentioned degassed mixed solution into a freeze-drying mold, and put it into a freeze-dryer for freeze-dry...

Embodiment 2

[0068] (1) Preparation of collagen solution

[0069] Adjust the temperature of the cooling water bath in the reactor to 4°C, add collagen and 0.1M citric acid solution, stir until dissolved, and prepare the solution with a final concentration of 5.5 mg / ml.

[0070] (2) Preparation of polymer solution

[0071] Add high molecular polymer (chitosan, molecular weight is 160kDa) and 0.1M citric acid solution in a sealed glass bottle, put it in a 55°C water bath, stir until dissolved and cool to room temperature, the final concentration of the prepared solution is 5.5mg / ml .

[0072] (3) mixed solution

[0073] The above two solutions were stirred and mixed in a reaction kettle at 5° C., and the final collagen-macromolecular polymer mass ratio was 6:1. Thereafter degassed under vacuum for 30 minutes.

[0074] Degassing, freeze-drying and heat treatment were carried out in the manner of Example 1.

[0075] The final collagen composite product was obtained with a measured moistur...

Embodiment 3

[0077] (1) Preparation of collagen solution

[0078] Adjust the temperature of the cooling water bath of the reaction kettle to 8°C, add collagen and 0.12M oxalic acid solution, stir until dissolved, and prepare the solution with a final concentration of 7.5mg / ml.

[0079] (2) Preparation of polymer solution

[0080] Add high molecular polymer (xanthan gum, molecular weight 160Da) and 0.12M oxalic acid solution into a sealed glass bottle, put it in a 60°C water bath, stir until dissolved and cool to room temperature, the final concentration of the prepared solution is 7.5mg / ml.

[0081] (3) mixed solution

[0082] The above two solutions were stirred and mixed in a reaction kettle at 5°C, and the final collagen-polymer mass ratio was 2:1.

[0083] Degassing, freeze-drying and heat treatment were carried out in the manner of Example 1.

[0084] The final collagen composite product was obtained with a measured moisture content of 0.8% by weight.

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Abstract

The invention relates to the technical field of biological materials, in particular to a collagen composite material and a preparation method and application thereof. The collagen composite material comprises a high-molecular polymer and collagen dispersed in the high-molecular polymer, and the weight ratio of the high-molecular polymer to the collagen is 1: (0.01-20). The collagen composite material provided by the invention can effectively reduce the degradation efficiency of collagen, and has good mechanical properties.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a collagen composite material and its preparation method and application. Background technique [0002] Collagen is the most abundant protein in the human body, accounting for about 25%-35% of the total protein in the body. Because of its excellent low immunogenicity, it is often used as a material for the preparation of tissue engineering scaffolds for the repair of damaged tissues. However, after the collagen matrix is ​​implanted into the human body, it has the disadvantages of fast degradation rate and inability to maintain structural stability. For this reason, the preparation of collagen matrix often uses chemical cross-linking agents to modify the collagen matrix to prolong its degradation time in vivo. Although the use of chemical cross-linking agents can effectively adjust the degradation time of the collagen matrix, the chemical cross-linking agents used are cyto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L71/02C08J5/18A61L27/48A61L27/58
CPCC08L89/00C08J5/18A61L27/48A61L27/58C08J2389/00C08J2471/02C08L71/02
Inventor 吴斯倞全丹毅
Owner 江苏集萃新型药物制剂技术研究所有限公司