Method for displaying character information on surface of cell

A text information, cell surface technology, applied in the field of information storage, can solve the problems of data can not be recovered, no protein display and other problems, to save the extraction of DNA or protein, save the tedious process effect

Pending Publication Date: 2021-12-03
上海国龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but does not display the protein on the cell surface
If only DNA is used as the

Method used

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  • Method for displaying character information on surface of cell
  • Method for displaying character information on surface of cell
  • Method for displaying character information on surface of cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 A method for displaying textual information in the form of protein on the surface of Pichia pastoris cells and its application, including the following steps:

[0019] (1) Convert the English name "YOUMINZHU" into amino acid information (SEQ ID No.1) as shown in the table below, with one letter for every two amino acid codes, or other coding methods, such as three or more amino acid codes for one letter;

[0020] The coding table that present embodiment adopts is as follows:

[0021]

[0022]

[0023] (2) Convert amino acid information into DNA information (SEQ ID No.2);

[0024] (3) The sequence (SEQ ID No.3) of the improved anchor protein, the name information sequence (SEQ ID No.2) and the β-galactosidase sequence (SEQ ID No.4) are fused into a gene (SEQ ID No.4) ID No.5), the two ends of the fusion gene contain carrier homology arm sequences, and the DNA information sequence serves as the connecting peptide between the anchor protein and the β-g...

Embodiment 2

[0049] Embodiment 2 A method for displaying textual information in the form of protein on the surface of Pichia pastoris cells and its application, including the following steps:

[0050] (1) Convert the English name "YOUMINZHU" into amino acid information (SEQ ID No.1);

[0051] (2) Convert amino acid information into DNA information (SEQ ID No.2);

[0052] (3) The improved anchor protein sequence (SEQ ID No.3), name information sequence (SEQ ID No.2), connecting peptide sequence (SEQ ID No.6) and β-galactosidase sequence (SEQ ID No.4) is fused into a gene (SEQ ID No.7), the two ends of the fusion gene contain carrier homology arm sequences, and the DNA information sequence is used as a part of the connecting peptide between the anchor protein and the β-galactosidase sequence;

[0053] (4) Chemically synthesize the fusion sequence of the anchor protein sequence shown in SEQ ID No.7, the DNA message sequence, the connecting peptide sequence and the β-galactosidase sequence. ...

Embodiment 3

[0075] Embodiment 3 A method for displaying text information in the form of protein on the surface of Pichia pastoris cells and its application, including the following steps:

[0076] (1) Convert the English name "YOUMINZHU" into amino acid information (SEQ ID No.1);

[0077] (2) Convert amino acid information into DNA information (SEQ ID No.2);

[0078] (3) Chemically synthesize the sequence of the improved anchor protein (SEQ ID No.3), and add the carrier homology arm sequence to the front end;

[0079] (4) Chemically synthesize the fusion sequence (SEQ ID No.8) of the name information sequence and the connecting peptide sequence, and add homology arm sequences before and after;

[0080] (5) Chemically synthesize the improved β-galactosidase sequence (SEQ ID No.4), and add the carrier homology arm sequence at the back end;

[0081] (6) The vector pPIC9K is digested with EcoRI and Not1, and the enzyme digestion system is as follows:

[0082]

[0083] (7) Recombine the ...

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PUM

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Abstract

The invention discloses a method for displaying character information on the surface of a cell, which comprises the following steps of: coding letters by using amino acid, and converting the character information expressed by the letters into an amino acid sequence; converting the amino acid sequence into a corresponding coding DNA sequence; fusing the coding DNA of the anchoring protein, the coding DNA sequence in the step (2) and the verification protein gene into a fusion gene, and constructing the fusion gene into a vector; transforming the vector into a pichia pastoris competent cell; and checking the expression condition of the verification protein in the pichia pastoris. Protein with information is displayed on the surface of a pichia pastoris cell. The method has the advantages that the information can be read through DNA sequencing and can also be read through the amino acid sequence corresponding to the protein. Even if the DNA is damaged, the reading of the information is still not influenced.

Description

technical field [0001] The invention relates to a method for displaying text information on the cell surface in the form of protein, and belongs to the technical field of information storage. Background technique [0002] The existing DNA information storage technology only uses DNA or protein as the medium for information storage. But it doesn't display the protein on the cell surface. If only DNA is used as the information storage medium, if the DNA is damaged, the data cannot be recovered. If the protein is used as the medium for information storage, but the protein is not displayed on the cell surface, the protein can only be distributed inside the cell, and the information cannot be deciphered on the cell surface, and the DNA or protein inside the cell can only be extracted by breaking the cell to decipher the information. Contents of the invention [0003] The purpose of the present invention is to provide a method for displaying text information on the cell surfa...

Claims

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Application Information

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IPC IPC(8): C12N15/81C12N15/62C12N1/19C12R1/84
CPCC12N15/815C12N9/2471C07K14/39C12Y302/01023C07K2319/00C12N2800/102
Inventor 朱佑民于婷婷张慧
Owner 上海国龙生物科技有限公司
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