Supercharge Your Innovation With Domain-Expert AI Agents!

Kit for determining specific growth factor

A growth factor and specific technology, applied in the field of medical testing, to achieve the effect of improving detection performance and measurability

Pending Publication Date: 2021-12-03
ZYBIO INC
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, by adding a composition of surfactants and salts, the problem of subsequent sample precipitation after adding organic solvents is solved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for determining specific growth factor
  • Kit for determining specific growth factor
  • Kit for determining specific growth factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 TSGF detection kit

[0030] The specific growth factor (TSGF) detection kit of the present invention includes two liquid components of reagent R1 and reagent R2 which are independent of each other.

[0031] 1. Preparation of Reagent R1

[0032] Prepare according to the following formula, stir and mix thoroughly, and store at 2-8°C.

[0033]

[0034] 2. Preparation of Reagent R2

[0035] Prepare according to the following formula, stir and mix thoroughly, and store at 2-8°C.

[0036]

Embodiment 2

[0037] The usage method of embodiment 2 kit

[0038] In this example, a fully automatic biochemical analyzer (Hitachi 7180) was used together with the kit of the present invention for sample detection.

[0039] (1) Instrument parameter setting

[0040]

[0041] (2) Measurement plan

[0042]

[0043]

[0044] (3) Calculation method

[0045] Using the two-point linear calibration mode, the linear function is used as the calculation mode, and the dose / response curve is made according to the value of the calibrator and the absorbance change value, and the content of the specific growth factor in the sample can be calculated on the dose / response curve according to the absorbance change value out.

[0046] Among them, the detection principle of the present invention is: the mixture in the kit is mixed with the specific growth factors (specific glycolipids with amino groups, metabolites of glycoproteins and free α- Substances such as amino acids) for superposition coupli...

Embodiment 3

[0047] The performance test of embodiment 3 kits

[0048] In order to verify each performance of the kit of the present invention, a total of 3 groups of kits are set for performance verification:

[0049] Group A: the kit prepared in Example 1 of the present invention;

[0050] Group B: specific growth factor assay kit (chemical method) (Beijing Jiuqiang);

[0051] Group C: kits obtained according to the method described in Example 1 of the patent CN104515768B specification.

[0052] Among them, the kits of group C all use the existing reagents in the laboratory during the preparation process (that is, the same components are involved in group A and group C, for example, the chromogenic agent ninhydrin all adopts the same batch of products from the same manufacturer). The kit was tested according to the usage method described in Example 2, group B was tested according to its instructions, and group C was tested according to the usage method in the example.

[0053] (1) Accur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for determining a specific growth factor, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a buffer solution, a surfactant 1, a surfactant 2, an inorganic salt and an organic solvent, and the reagent R2 comprises a buffer solution, a color developing agent and a preservative. By adding a certain amount of organic solvent (DMSO) into the detection reagent, forward proceeding of ninhydrin chromogenic reaction can be effectively promoted, so that the chromogenic reaction is close to complete reaction. Meanwhile, by adding the composition of the surfactant and the salt, the problem of subsequent sample precipitation caused by adding the organic solvent is solved. Through the cooperation of the improvement measures and the reaction system provided by the invention, the detection performance of the kit is effectively improved, and the detection performance comprises precision and the measurability of a to-be-detected object in a linear range.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a kit for measuring specific growth factors. Background technique [0002] Specific Growth Factor (SGF) is a special class of substances, including carbohydrates, amino acids and their metabolites, which have a characteristic that when a tumor occurs, the content of specific growth factors in the blood increases. High, it can stimulate the formation of malignant tumor angiogenesis, and after angiogenesis, it can provide sufficient nutrition for the tumor tissue, thereby promoting the rapid growth of the tumor. Specific growth factors will increase significantly in the early stage of tumors. Clinically, the content of specific growth factors in serum can be measured as an effective reference for early detection of tumors, changes in patients' conditions, and postoperative monitoring. [0003] Currently known detection methods for SGF include enzyme-linked immunoassay (ELISA) and co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/92G01N21/78
CPCG01N33/68G01N33/6806G01N33/92G01N21/78Y02A50/30
Inventor 马腾飞杨焜诚刘霖芮海涛
Owner ZYBIO INC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com