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Method for improving extraction rate of chondroitin sulfate prepared from tilapia mossambica skull

A chondroitin sulfate and tilapia technology, applied in the field of bioengineering, can solve the problems of complex steps, long production cycle and high time requirements, and achieve the effects of improving extraction rate, efficient extraction and mild reaction conditions

Active Publication Date: 2021-12-10
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the alkali-salt method will pollute the environment and degrade chondroitin sulfate; ultrasonic assistance requires high time and is prone to impurities, and the efficiency of neutral salt extraction of chondroitin sulfate is low
The separation and purification methods of chondroitin sulfate include chromatography, electrophoresis ultrafiltration, anion exchange resin method, etc., but these methods have problems such as complicated steps, long production cycle and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0024] (1) Tilapia skulls were steamed at 100°C for 30 minutes to remove excess muscle and other impurities, soaked in ethanol overnight, dried in an oven at 65°C for 8 hours, crushed into powder, and stored at -80°C for later use.

[0025] (2) Take 10g of the fish skull powder prepared in (1), add 50mM Na 2 CO 3 solution, adjust the pH=7.0, then add 200 μL Savinase 16L, stir in a water bath at 55°C for 4 hours, and inactivate the enzyme at 100°C for 10 minutes. Cool to room temperature to obtain the first enzymolysis solution.

[0026] (3) Add 5.4 mg / ml 2709 alkaline protease to the first enzymolysis solution obtained in (2), react in a water bath at 50°C for 2 hours, and inactivate the enzyme at 100°C for 10 minutes. Cool to 4°C, centrifuge at 8000r / min for 20min, collect the supernatant, and obt...

Embodiment 2

[0031] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0032] The operation process is the same as that in Example 1, except that the mass-volume ratio of tilapia skull powder to Savinase 16L in step (2) is 1:15 in g:μL; The mass-to-volume ratio of the solution is 5:1 in mg:mL.

Embodiment 3

[0034] This example provides a test for preparing chondroitin sulfate by applying a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skull.

[0035]The operation process is the same as that in Example 1, except that the mass-to-volume ratio of tilapia skull powder to Savinase 16L in step (2) is 1:25 in g:μL; The mass-to-volume ratio of the solution is 6:1 in mg:mL.

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PUM

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Abstract

The invention provides a method for preparing chondroitin sulfate from tilapia mossambica skull. The method comprises the following steps: performing enzymolysis on tilapia mossambica skull powder by adopting Savinase 16L to obtain Savinase 16L enzymatic hydrolysate; carrying out secondary enzymolysis on the Savinase 16L enzymatic hydrolysate by using 2709 alkaline protease; and separating and purifying with ethanol and cetylpyridinium chloride to obtain the chondroitin sulfate. According to the method, a composite enzymolysis method of Savinase 16L and 2709 alkaline protease is adopted, efficient extraction of chondroitin sulfate from the tilapia mossambica skull powder is effectively achieved, purification of chondroitin sulfate is further achieved through ethyl alcohol and cetylpyridinium chloride, and high-value utilization of tilapia mossambica by-products is achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for improving the extraction rate of chondroitin sulfate prepared from tilapia skulls. Background technique [0002] Chondroitin Sulfate (CS) is a highly sulfated glycosaminoglycan composed of alternating D-glucuronic acid and N-acetyl-D-galactosamine-linked disaccharide units. According to the number and types of sulfuric acid groups attached to different positions of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc), there are many isomers of chondroitin sulfate. According to existing studies, chondroitin sulfate has various biological activities such as anti-inflammation, anti-oxidation, anti-coagulation, hypolipidemic and anti-tumor, and effectively inhibits thrombus formation, hinders atherosclerotic plaque formation and reduces Blood lipid index, thereby preventing cardiovascular disease. In addition, chondroitin sulfate can also be used as a food ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069C12P19/04C12Y304/21062C12N9/54C12N9/6408
Inventor 钟赛意左格格陈菁陈建平李瑞宋兵兵陈康健刘晓菲贾学静
Owner GUANGDONG OCEAN UNIVERSITY
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