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A kind of goosegrass biocontrol bacteria

A technology of oxtenoid grass and biocontrol bacteria, applied in the directions of microorganisms, fungi, biocides, etc., can solve the problems of aggravating environmental pollution of oxtenda grass, affecting the growth of other crops, agricultural ecosystem functions and structural threats, etc.

Pending Publication Date: 2021-12-10
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The grass has strong adaptability, fast growth, strong fecundity, trampling resistance, and strong competitive advantage, which seriously affects the growth of other crops and poses a serious threat to the function and structure of the agricultural ecosystem
[0003] At present, the use of chemical herbicides is the main control measure for goosegrass. However, with the extensive use of chemical herbicides, the problem of drug resistance and environmental pollution of goosegrass is increasing. The efficient and safe control of goosegrass is facing severe challenges.

Method used

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  • A kind of goosegrass biocontrol bacteria
  • A kind of goosegrass biocontrol bacteria
  • A kind of goosegrass biocontrol bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, purification and identification of herbicidal fungi

[0023] 1. Isolation and purification of goosegrass pathogenic bacteria: select diseased leaf samples, use conventional tissue separation method to isolate pathogenic bacteria, wash the diseased leaves with clean water and cut out 4mm×4mm disease-health junction tissue, treat with 75% ethanol for 30s, 0.1 Sterilize with %mercuric chloride for 1-2min, rinse with sterile water 3 times, inoculate on PDA medium, culture at 25°C for 5 days, purify the obtained strains through single spores, store them on PDA slant and filter paper, and place them at 4°C respectively and store in -20°C refrigerator for later use.

[0024] 2. Pathogenicity identification of goosegrass pathogenic bacteria: use in vitro inoculation of mycelium block and in vivo inoculation of conidia liquid, mycelium block inoculation: select disease-free leaves of the same size from healthy goosegrass plants at the three-leaf stage 20 p...

Embodiment 2

[0026] Embodiment 2: biological characteristics of goosegrass anthracnose

[0027] 1. Effect of culture medium on strain growth and sporulation: inoculate pathogenic bacteria on PDA, PSA, OTA, OMA, CA, MBA culture plates, culture in dark at 25°C, observe colony shape, color, conidia and appressoria feature.

[0028] 2. The effect of temperature on the strain NJC-16: take a mycelium block with a diameter of 6mm, connect it to the center of the PDA plate, and cultivate it under constant temperature conditions of 5, 10, 15, 20, 25, 30, and 35°C, and observe For mycelium growth, the diameter of the colony was measured by the cross method after 5 days, and each treatment was repeated 5 times.

[0029]3. The effect of light on the strain NJC-16: Connect the mycelium block of pathogenic bacteria with a diameter of 6mm to the center of the PDA plate, place the plate in an artificial climate incubator with continuous light, 12h alternating light and dark, and continuous darkness, at a...

Embodiment 3

[0032] Example 3: Determination of pathogenicity of bacterial strain NJC-16

[0033] The strain NJC-16 was inoculated in PDB medium, shaken at 25°C and 220r / min for 7 days, and the spores were collected and prepared at a concentration of 1×10 6 Individual / mL spore suspension (containing 0.05% Tween 80). Spray 10mL of the spore suspension in each pot on weed plants such as barnyardgrass, crabgrass and retroverted amaranthus at the 3-leaf stage. After 2 days of moist cultivation in the dark at 28°C, they were cultured in 16h / 8h light / dark. Take the plants sprayed with 0.05% Tween 80 as the control, and observe the disease state of the plants after 7 days. Each treatment was repeated 3 times.

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Abstract

The invention discloses a goosegrass biocontrol bacterium, which collects goosegrass natural diseased plants, adopts potato dextrose agar medium (PDA), and obtains various bacterial strains through indoor tissue separation, culture and purification, and is verified by Koch's law Among the pathogenic bacteria, a pathogenic fungus (NJC-16) with strong pathogenicity to goosegrass was finally obtained; it was preserved in the China Center for Type Culture Collection, and the preservation number: CCTCC NO.M2020688. The goosegrass anthracnose bacterium of the invention has strong specificity and relatively rapid pathogenicity, and can form obvious lesion spots on leaves three days after inoculation, and with the expansion of the lesion spots, the purpose of effectively preventing and controlling goosegrass is achieved. The fungus is safe to corn, wheat and rice, has strong specificity, and has potential for further development.

Description

technical field [0001] The invention belongs to the technical field of weed biological control, in particular to a biocontrol fungus (Colletotrichum eleusines) capable of infecting and controlling goosegrass. Background technique [0002] Goosegrass is a vicious weed in farmland. It is distributed in various provinces in the north and south of China and in temperate and tropical regions of the world. It mainly harms food crops such as corn, cotton, and soybeans, and economic crops such as vegetables and fruit trees. The grass has strong adaptability, fast growth, strong fecundity, trampling resistance, and strong competitive advantage, which seriously affects the growth of other crops and poses a serious threat to the function and structure of the agricultural ecosystem. [0003] At present, the use of chemical herbicides is the main control measure for goosegrass. However, with the extensive use of chemical herbicides, the problem of goosegrass drug resistance and environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P13/02C12R1/645
Inventor 顾琼楠李儒海褚世海黄启超陈安安
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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