Fermentation medium of animal bifidobacterium and application thereof
A technology of animal bifidobacteria and fermentation medium, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of lack of efficacy comparison, lack of effective efficacy data of probiotics, etc., and achieve good stability and significant barrier repair effect. Effect
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Embodiment 1-1
[0103] The preparation of embodiment 1-1 fermentation medium
[0104] The carbon source is glucose 4.20g / L,
[0105] Malt extract 0.5g / L, calcium chloride 0.2g / L,
[0106] The nitrogen source is tryptone and yeast powder, tryptone 3.50g / L, yeast powder 2.90g / L,
[0107] Inorganic salts are anhydrous sodium acetate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate trihydrate, wherein, anhydrous sodium acetate 2.00g / L, diammonium hydrogen citrate 1.80g / L, magnesium sulfate heptahydrate 0.55g / L, manganese sulfate monohydrate 0.25g / L, dipotassium hydrogen phosphate trihydrate 2.50g / L,
[0108] Surfactant is Tween 80 0.90ml / L,
[0109] Antioxidant is L-cysteine hydrochloride 0.60g / L,
[0110] Weigh the medium according to the above formula (weigh the glucose separately, put it in a sterilizing pot for sterilization at 118°C for 15 minutes, add it to the fermenter before inoculation), add it to the f...
Embodiment 1-2
[0111] The preparation of embodiment 1-2 fermentation medium
[0112] The carbon source is glucose 2.00g / L,
[0113] Malt extract 0.5g / L, calcium chloride 0.2g / L,
[0114] The nitrogen source is tryptone and yeast powder, tryptone 2.00g / L, yeast powder 1.50g / L,
[0115] Inorganic salts are anhydrous sodium acetate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate trihydrate, wherein, anhydrous sodium acetate 1.00g / L, diammonium hydrogen citrate 1.00g / L, magnesium sulfate heptahydrate 0.10g / L, manganese sulfate monohydrate 0.10g / L, dipotassium hydrogen phosphate trihydrate 1.00g / L,
[0116] Surfactant is Tween 80 0.30ml / L,
[0117] The antioxidant is 0.20 g / L of L-cysteine hydrochloride, and the sterilization method is the same as in Example 1-1.
Embodiment 1-3
[0118] The preparation of embodiment 1-3 fermentation medium
[0119] The carbon source is glucose 9.00g / L,
[0120] Malt extract 0.5g / L, calcium chloride 0.2g / L,
[0121] The nitrogen source is tryptone and yeast powder, tryptone 7.00g / L, yeast powder 5.50g / L,
[0122] Inorganic salts are anhydrous sodium acetate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate trihydrate, wherein, anhydrous sodium acetate 4.00g / L, diammonium hydrogen citrate 4.00g / L, magnesium sulfate heptahydrate 1.80g / L, manganese sulfate monohydrate 0.75g / L, dipotassium hydrogen phosphate trihydrate 3.75g / L,
[0123] Surfactant is Tween 80 1.75ml / L,
[0124] Antioxidant is L-cysteine hydrochloride 1.20g / L, and the sterilization method is the same as that of Example 1-1.
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