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Bifidobacterium animalis fermentation filtrate as well as preparation method and application thereof

A technology of animal bifidobacterium and fermentation liquid, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of lack of effective efficacy data and comparison of efficacy of probiotics, and achieve significant barrier repair efficacy Effect

Active Publication Date: 2021-12-10
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 2. Probiotics used in the field of cosmetics lack effective efficacy data, and there is no comparison of efficacy before and after use;

Method used

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  • Bifidobacterium animalis fermentation filtrate as well as preparation method and application thereof
  • Bifidobacterium animalis fermentation filtrate as well as preparation method and application thereof
  • Bifidobacterium animalis fermentation filtrate as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] The preparation of embodiment 1 bifidobacterium animal fermentation filtrate:

[0131] 1. Seed solution culture:

[0132] (1) The formula of the seed liquid medium is as follows: glucose 20.00g / L, tryptone 10.00g / L, yeast powder 5.00g / L, anhydrous sodium acetate 2.00g / L, diammonium hydrogen citrate 2.00g / L, seven Magnesium sulfate water 0.58g / L, manganese sulfate monohydrate 0.25g / L, Tween 80 1.00ml / L, dipotassium hydrogen phosphate trihydrate 2.60g / L and L-cysteine ​​hydrochloride 0.50g / L.

[0133] (2) Sterilization: add peptone, yeast powder, magnesium sulfate heptahydrate, manganese sulfate monohydrate, Tween 80, diammonium hydrogen citrate, dipotassium hydrogen phosphate trihydrate, etc. into purified water according to the formula of the seed liquid medium Adjust the pH to 6.2, divide the packaging, prepare the mother liquor according to a certain concentration of glucose, separate the packaging, sterilize at 115°C for 20 minutes, and add the glucose into the medi...

Embodiment 2

[0161] The preparation of embodiment 2 bifidobacterium animal fermentation filtrate

[0162] 1. Seed liquid culture

[0163] Seed liquid culture medium formula, sterilization and cultivation are identical with embodiment 1.

[0164] 2. Fermentation culture:

[0165] (1) The formula of the fermentation medium is as follows: the carbon source is glucose 2.00g / L,

[0166] Malt extract 0.5g / L, calcium chloride 0.2g / L,

[0167] The nitrogen source is tryptone and yeast powder, tryptone 2.00g / L, yeast powder 1.50g / L,

[0168] Inorganic salts are anhydrous sodium acetate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate trihydrate, wherein, anhydrous sodium acetate 1.00g / L, diammonium hydrogen citrate 1.00g / L, magnesium sulfate heptahydrate 0.10g / L, manganese sulfate monohydrate 0.10g / L, dipotassium hydrogen phosphate trihydrate 1.00g / L,

[0169] Surfactant is Tween 80 0.30ml / L,

[0170] Antioxidant i...

Embodiment 3

[0175] The preparation of embodiment 3 animal bifidobacterium fermentation filtrate

[0176] 1. Seed liquid culture

[0177] Seed liquid culture medium formula, sterilization and cultivation are identical with embodiment 1.

[0178] 2. Fermentation culture:

[0179] (1) The formula of the fermentation medium is as follows: the carbon source is glucose 9.00g / L,

[0180] Malt extract 0.5g / L, calcium chloride 0.2g / L,

[0181] The nitrogen source is tryptone and yeast powder, tryptone 7.00g / L, yeast powder 5.50g / L,

[0182]Inorganic salts are anhydrous sodium acetate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and dipotassium hydrogen phosphate trihydrate, wherein, anhydrous sodium acetate 4.00g / L, diammonium hydrogen citrate 4.00g / L, magnesium sulfate heptahydrate 1.80g / L, manganese sulfate monohydrate 0.75g / L, dipotassium hydrogen phosphate trihydrate 3.75g / L,

[0183] Surfactant is Tween 80 1.75ml / L,

[0184] Antioxidant is...

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Abstract

The invention discloses a bifidobacterium animalis fermentation filtrate as well as a preparation method and application thereof. The fermentation filtrate is prepared by adopting a method comprising the following steps: inoculating bifidobacterium animalis into a seed culture medium for culturing to obtain a seed solution; inoculating the seed solution into a fermentation culture medium containing malt extract, calcium chloride and glucose, and fermenting to obtain a fermentation solution; and centrifuging and filtering the fermentation solution to obtain bifidobacterium animalis fermentation filtrate. The fermented filtrate has a remarkable barrier repairing effect, compared with a blank control without the product, the water content of the cuticle of the skin is increased by 9%-10%, the transdermal water loss amount is reduced by 5%, and the fermented filtrate can be applied to the field of cosmetics and used for research and development of skin barrier repairing products.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a bifidobacterium animal fermentation filtrate, a preparation method and an application thereof. Background technique [0002] Probiotics generally refer to microorganisms that have beneficial effects on humans and animals. The research on the probiotic function of probiotics has been expanded to various aspects related to health outside the intestinal tract, and it is a hot spot in current basic research. Its applications can be seen everywhere in life, including the food industry, medicine field, and skin care field. [0003] Human skin is colonized by about one billion microorganisms per square centimeter. These microorganisms secrete antimicrobial peptides or free fatty acids to prevent skin colonization of diseased bodies, thereby protecting the health of the skin. [0004] At present, the research on the functional characteristics of probiotics is relative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K8/99A61K8/9794A61Q19/00C12R1/01
CPCC12N1/20A61K8/99A61K8/9794A61Q19/00A61K2800/85
Inventor 张天萌刘金钊朱倩倩张由恒余济源温亮亮张晓鸥王玉玲谢文娟郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD