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Method for simultaneously determining content of various components of traditional Chinese medicine for treating liver diseases

A traditional Chinese medicine, content technology, applied in the direction of measurement device, material separation, analysis of materials, etc., can solve the problems of few components, cumbersome processing, long analysis time, etc., and achieve the effect of saving measurement time, easy operation, and accurate measurement results.

Pending Publication Date: 2021-12-17
ZHEJIANG WECOME MEDICINE IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its "Chinese Pharmacopoeia" 2015 edition records that kudzu root is based on puerarin, danshen is based on tanshinone IIA, red peony is based on paeoniflorin, notoginseng is based on notoginsenoside R 1 Astragalus is used as an index, and astragaloside IV is used as an index for Astragalus membranaceus, but when using high-performance liquid chromatography to measure, the contents of astragaloside IV, puerarin, and paeoniflorin are separately measured, and there are few components to be measured. For the quantitative analysis of these three components It is difficult to truly control the product quality of traditional Chinese medicine in detection, and it is impossible to completely eliminate the phenomenon of adulteration and inferiority of traditional Chinese medicine, and it cannot objectively reflect and evaluate the consistency of the quality of traditional Chinese medicine, and the sample pretreatment is cumbersome, the analysis time is long, and the operability is not strong

Method used

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  • Method for simultaneously determining content of various components of traditional Chinese medicine for treating liver diseases
  • Method for simultaneously determining content of various components of traditional Chinese medicine for treating liver diseases
  • Method for simultaneously determining content of various components of traditional Chinese medicine for treating liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] S1. Accurately weigh 4.0 g of traditional Chinese medicine for treating liver diseases with the batch number of 1805311, grind it finely, accurately weigh it, put it in a conical flask, add 25 mL of 50% methanol solution, seal it tightly, weigh it, ultrasonically treat it for 30 minutes, let it cool, and then Weigh the weight, make up the lost weight with 50% methanol solution, shake well, filter through a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain the yellow beetle soft liver solution.

[0079] S2, accurately weigh gallic acid, puerarin, paeoniflorin, notoginseng saponin R 1 , astragaloside IV and tanshinone IIA, add 50% methanol solution to make the reference substance stock solution, accurately draw a certain amount of each reference substance stock solution and put it in the same measuring bottle, add 50% methanol solution to dilute to the mark, and obtain the reference substance solution.

[0080] S3, absorbing the Huangjia Ruangan sol...

Embodiment 2

[0109] Precisely weigh 4g of Chinese medicine for the treatment of liver diseases with batch number 1805312, and measure according to the steps in Example 1. The difference from Example 1 is that the conditions of high performance liquid chromatography are:

[0110] Column: Waters Symmetry C 18 Chromatographic column, specification 250mm*4.6mm, 5μm;

[0111] Mobile phase: mobile phase A is methanol, mobile phase B is 0.08% glacial acetic acid, gradient elution is used, and the mobile phase ratios are all volume percentages:

[0112] 0-25min, mobile phase A is 5%-30%, mobile phase B is 95%-70%;

[0113] 25-50min, mobile phase A is 30%-35%, mobile phase B is 70%-65%;

[0114] 50-65min, mobile phase A is 35%-45%, mobile phase B is 65%-55%;

[0115] 65-95min, mobile phase A is 45%-95%, mobile phase B is 55%-5%;

[0116] 95-105min, mobile phase A is 95%, mobile phase B is 5%;

[0117] Flow rate: 0.8mL / min;

[0118] Column temperature: 25°C;

[0119] Detection wavelength: 270...

Embodiment 3

[0125] Precisely weigh 4g of Chinese medicine for the treatment of liver diseases with batch number 1805313, and measure according to the steps in Example 1. The difference from Example 1 is that the conditions of high performance liquid chromatography are:

[0126] Column: Waters Symmetry C 18 Chromatographic column, specification 250mm*4.6mm, 5μm;

[0127] Mobile phase: mobile phase A is methanol, mobile phase B is 0.12% glacial acetic acid, gradient elution is used, and the mobile phase ratios are all volume percentages:

[0128] 0-25min, mobile phase A is 5%-30%, mobile phase B is 95%-70%;

[0129] 25-50min, mobile phase A is 30%-35%, mobile phase B is 70%-65%;

[0130] 50-65min, mobile phase A is 35%-45%, mobile phase B is 65%-55%;

[0131] 65-95min, mobile phase A is 45%-95%, mobile phase B is 55%-5%;

[0132] 95-105min, mobile phase A is 95%, mobile phase B is 5%;

[0133] Flow rate: 0.9mL / min;

[0134] Column temperature: 27°C;

[0135] Detection wavelength: 270...

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Abstract

The invention discloses a method for simultaneously determining the contents of various components of a traditional Chinese medicine for treating liver diseases. According to the method, a high performance liquid chromatography method is adopted, and a chromatographic column is a Waters Symmetry C18 chromatographic column; a mobile phase A is methanol, a mobile phase B is 0.1% glacial acetic acid, gradient elution is carried out for 0-25 min and adopting 5%-30% of A and 95%-70% of B, gradient elution is carried out for 25-50 minand adopting 30%-35% of A and 70%-65% of B, gradient elution is carried out for 50-65 min and adopting 35%-45% of A and 65%-55% of B, gradient elution is carried out for 65-95 min and adopting 45%-95% of A and 55%-5% of B, gradient elution is carried out for 95-105 min and adopting 95% of A and 5% of B, and the flow rate is 1.0 mL / min; the column temperature is 30 DEG C, and the detection wavelength is 270 nm; and the sample size is 5 microliters. The method can be used for qualitatively and quantitatively analyzing the content of various components of the traditional Chinese medicine for treating liver diseases simultaneously, saves the determination time and is simple and convenient to operate.

Description

technical field [0001] The invention relates to the field of quality control methods of traditional Chinese medicine preparations, in particular to a method for simultaneously measuring the contents of multiple components of the traditional Chinese medicine, and the method is used to determine the contents of each component contained in the traditional Chinese medicine for treating liver diseases. Background technique [0002] As a compound preparation, traditional Chinese medicine for the treatment of liver disease belongs to the sixth category of traditional Chinese medicine in the research and development of new drugs. The specific composition and preparation method are as described in the Chinese patent application CN101214288B "A Traditional Chinese Medicine Composition for Treating Liver Disease and Its Preparation Method". Its "Chinese Pharmacopoeia" 2015 edition records that kudzu root is based on puerarin, danshen is based on tanshinone IIA, red peony is based on pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88G01N30/06G01N30/34
CPCG01N30/02G01N30/88G01N30/06G01N30/34G01N2030/884Y02A50/30
Inventor 张保献高进姜志宏曹晖王宗权李翠朱国元
Owner ZHEJIANG WECOME MEDICINE IND
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