Production process for increasing content of astaxanthin in haematococcus pluvialis by using xanthophyll

Abstract

The invention discloses a production process for increasing the content of astaxanthin in haematococcus pluvialis by using xanthophyll, and aims to solve the technical problems that in the prior art, the content of the astaxanthin in the haematococcus pluvialis is not high when the astaxanthin in the haematococcus pluvialis is extracted and produced, and processing raw materials are not completely extracted during processing. According to the production process, haematococcus pluvialis cells are inoculated for multiplication culture, a multiplication culture material is subjected to induction culture, the haematococcus pluvialis cells are induced to accumulate the astaxanthin, the haematococcus pluvialis is used for preparing content materials, and the content of the astaxanthin in the haematococcus pluvialis is increased by increasing the added xanthophyll; meanwhile, by optimizing the processing process, processing and production are better carried out, the working speed and efficiency are improved, astaxanthin loss caused by a saponification process is overcome, and the yield of high-purity astaxanthin ester is high; and the process is simple and concise, a used separation and purification solvent meets the requirements of food additives, and the process is simple to operate and easy to industrialize.

Description

technical field

[0001] The invention belongs to the field of astaxanthin production, and in particular relates to a production process for increasing the content of astaxanthin in Haematococcus pluvialis by lutein. Background technique

[0002] Astaxanthin is a ketone carotenoid, red solid powder, fat-soluble, insoluble in water, soluble in organic solvents, widely present in the biological world, especially in aquatic animals such as shrimp, crab, fish and birds In feathers, it plays the role of color development and has a strong antioxidant capacity. It can remove nitrogen dioxide, sulfide, disulfide, etc., and can also reduce lipid peroxidation, effectively inhibiting lipid oxidation caused by free radicals. Peroxidation, the main production methods of astaxanthin include artificial synthesis and biological acquisition. Synthetic astaxanthin is only expensive, while the biological source of natural astaxanthin is the most suitable for large-scale production, using Haemat...

Examples

Embodiment 1

[0029] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0030] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0031] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium for 5 days, adding a biological inducer for induction culture for 48 hours, and obtaining an induced culture, the inducer is the fermentation product of Bacillus subtilis of oat ;

[0032] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, remove the superna...

Embodiment 2

[0038] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0039] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0040] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium and culturing them for 3 days, adding a biological inducer for induction culture for 24 hours to obtain an induced culture, the inducer being the fermentation product of Bacillus subtilis of oat ;

[0041] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, r...

Embodiment 3

[0047] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0048] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0049] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium and culturing them for 3 days, adding a biological inducer for induction culture for 24 hours to obtain an induced culture, the inducer being the fermentation product of Bacillus subtilis of oat ;

[0050] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, r...

technical field

[0001] The invention belongs to the field of astaxanthin production, and in particular relates to a production process for increasing the content of astaxanthin in Haematococcus pluvialis by lutein. Background technique

[0002] Astaxanthin is a ketone carotenoid, red solid powder, fat-soluble, insoluble in water, soluble in organic solvents, widely present in the biological world, especially in aquatic animals such as shrimp, crab, fish and birds In feathers, it plays the role of color development and has a strong antioxidant capacity. It can remove nitrogen dioxide, sulfide, disulfide, etc., and can also reduce lipid peroxidation, effectively inhibiting lipid oxidation caused by free radicals. Peroxidation, the main production methods of astaxanthin include artificial synthesis and biological acquisition. Synthetic astaxanthin is only expensive, while the biological source of natural astaxanthin is the most suitable for large-scale production, using Haemat...

Examples

Embodiment 1

[0029] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0030] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0031] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium for 5 days, adding a biological inducer for induction culture for 48 hours, and obtaining an induced culture, the inducer is the fermentation product of Bacillus subtilis of oat ;

[0032] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, remove the superna...

Embodiment 2

[0038] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0039] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0040] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium and culturing them for 3 days, adding a biological inducer for induction culture for 24 hours to obtain an induced culture, the inducer being the fermentation product of Bacillus subtilis of oat ;

[0041] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, r...

Embodiment 3

[0047] This embodiment is a production process for improving the content of astaxanthin in Haematococcus pluvialis, and its steps are as follows:

[0048] Step 1: Inoculate Haematococcus pluvialis cells for expansion culture to obtain an expansion culture, induce culture for the expansion culture, induce the algae cells to accumulate astaxanthin, obtain an induction culture, and separate from the induction culture to obtain Haematococcus pluvialls, Haematococcus pluvialls expanded culture in expansion medium;

[0049] Step 2: Separating the algae cells from the expanded culture, transferring them to nitrogen-deficient BG11 medium and culturing them for 3 days, adding a biological inducer for induction culture for 24 hours to obtain an induced culture, the inducer being the fermentation product of Bacillus subtilis of oat ;

[0050] Step 3: Use Haematococcus pluvialis to prepare the content materials, put the collected fresh Haematococcus pluvialis into static sedimentation, r...