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Porcine circovirus type 2 Cap protein as well as coding gene and application thereof

A technology of porcine circovirus and coding gene, which is applied in the field of porcine circovirus type 2 Cap protein and its coding gene and its application, can solve the problems of less post-translational modification, high production cost, unfavorable purification, etc., and achieve reduction of production cost, The effect of huge social benefits and high economic value

Pending Publication Date: 2021-12-28
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The subunit vaccine produced by the baculovirus expression system is difficult to operate and the production cost is high, while the Escherichia coli expression system has little post-translational modification of the protein, and it is easy to form inclusion bodies, without the use of later purification

Method used

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  • Porcine circovirus type 2 Cap protein as well as coding gene and application thereof
  • Porcine circovirus type 2 Cap protein as well as coding gene and application thereof
  • Porcine circovirus type 2 Cap protein as well as coding gene and application thereof

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Embodiment Construction

[0022] In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other implementations obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

[0023] 1. Reagents used and their preparation

[0024] (1) LB (Luria-Bertani) liquid medium: take 10g peptone, 5g yeast powder, and 5g NaCl, dissolve in 800mLddH 2 O, 10M NaOH to adjust pH to 7.0, dilute to 1000mL, autoclave (121℃, 1.034×10 5 Pa) 20min, 4 ℃ preservation.

[0025] (2) LB (Luria-Bertani) solid medium: per 1000mL ddH 2 Add 15 g of agar powder to O, add agar powder to the LB liquid medium, autoclave, and store at 4°C.

[0026] (3) Reserve solution:

[0027] 10×YNB ...

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Abstract

The invention belongs to the field of genetic engineering in the biotechnological pharmaceutical industry, and particularly relates to a porcine circovirus type 2 Cap protein as well as a coding gene and application thereof. The amino acid sequence is as shown in SEQ ID NO: 2. The invention further provides an application of the porcine circovirus type 2 Cap protein in preparation of a porcine circovirus type 2 vaccine. According to the porcine circovirus type 2 Cap protein, the advantages of Cap and Pichia pastoris are well utilized, the coding gene of the porcine circovirus type 2 Cap protein can be efficiently expressed in the Pichia pastoris, the production cost is reduced, and the recombinant protein has very high economic value and huge social benefits when being applied to preparation of porcine circovirus vaccines.

Description

technical field [0001] The invention belongs to the field of genetic engineering in the biotechnology pharmaceutical industry, in particular to a porcine circovirus type 2 Cap protein and its encoding gene and application. Background technique [0002] At present, the most effective means of preventing PCV2 is injection of vaccine, and most of the commercialized PCV2 vaccines are inactivated vaccines. Although the application of commercial inactivated vaccines has played an important role in the effective prevention and control of PCV2 infection, the preparation of inactivated vaccine antigens has low virus titers, long production cycles, high production costs, and difficulty in culturing the virus. Inactivated vaccines cannot completely block the spread of PCV2, nor can they completely remove the infected virus from the body. Therefore, the subunit vaccine developed based on PCV2 structural protein Cap has attracted the attention of our research group due to its quality co...

Claims

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Application Information

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IPC IPC(8): C07K14/01C07K1/22C12N15/34C12N15/81A61K39/12A61P31/20
CPCC07K14/005C12N15/815A61K39/12A61P31/20C12N2800/22C12N2750/10022C12N2750/10034
Inventor 孙娜姜俊兵范阔海李宏全孙耀贵尹伟孙盼盼
Owner SHANXI AGRI UNIV
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