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Method for rapidly quantifying adulterated content of sodium bicarbonate in fresh milk

A technology for quantifying raw milk and sodium bicarbonate, applied in the field of food testing, can solve the problems of unfriendliness and sample damage to the environment, and achieve the effect of simple measurement process and good practical application value

Inactive Publication Date: 2021-12-31
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, bromothymol blue or rose red are commonly used in milk production to carry out color reaction to judge whether raw milk is mixed with alkaline substances. Although this method has high sensitivity, it will cause irreversible damage to the sample and is not friendly to the environment.

Method used

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  • Method for rapidly quantifying adulterated content of sodium bicarbonate in fresh milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Simulation of adulterated raw milk samples:

[0040] The raw milk samples were collected from Shanghai Dairy No. 4 Factory, and the indicators of protein, fat and carbohydrates of the raw milk met the standards when they left the factory.

[0041] Take an appropriate amount of fresh raw milk samples and add them to a pre-sterilized Erlenmeyer flask, fully wrap them to prevent the infection of external bacteria, and then store them in a constant temperature incubator at 37°C for 2 hours to obtain spoiled fresh milk samples, and add different The amount (0.1%-2.25%, w / w) of sodium bicarbonate solid was stirred thoroughly to obtain raw milk samples mixed with different sodium bicarbonate contents.

Embodiment 2

[0043] Detection of fast gas-phase electronic nose:

[0044] Accurately weigh 5g of the sample to be tested in a 20mL sample bottle, and then sequentially place it on the sample rack that comes with the instrument, so that its mechanical arm can detect the sample in an orderly and accurate manner. Set the sampling sequence through the software, and use the fast gas phase electronic nose To detect the volatile compounds of the samples to be tested, the detection conditions are as follows: the sample bottle is closed with a leak-proof cap and covered with a silicon / PTFE septum. The samples were incubated at 50°C for 20 min, then the autosampler injected 5000 µL of sample from the headspace into the GC at a rate of 125 µL / s, and the analytes were collected in the Tenax trap at 40°C for 50 s. After rapid heating, the analytes were separated and transferred to two parallel short GC columns (Restek, Center County, Pennsylvania, USA): non-polar column (MXT-5: 5% biphenyl, 95% methylp...

Embodiment 3

[0046] Data preprocessing and data set creation:

[0047] The spectra obtained from the two flash chromatography columns were combined and arranged according to the retention time, and the substances with different response values ​​but similar retention times were regarded as the same substance, and the peak areas with different retention times were used as independent variables. NaHCO 3 The actual content of the data set is established for the dependent variable, and the data set is randomly divided into a training set and a test set in a ratio of 7:3.

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Abstract

The invention discloses a method for rapidly quantifying the adulterated content of sodium bicarbonate in fresh milk. The method comprises the following steps: detecting metamorphic raw and fresh milk samples doped with NaHCO3 with different concentrations by utilizing FGC E-nose to obtain a chromatogram, and then establishing a data set by taking peak areas at different retention times as independent variables and taking the actual content of NaHCO3 as a dependent variable; randomly dividing the data set into a training set and a test set according to a ratio of 7: 3; and importing the training set into a random forest (RF) to train a prediction model, evaluating the performance of the model by using the test set, optimizing the parameters of the R-F model, and finally obtaining an RF model with excellent prediction performance. According to the RF model, for a test set, the decision coefficient R2 is 0.9587, the root-mean-square error is 0.0079, and in practical application, the decision coefficient R2 of a prediction set is equal to 0.9865, the root-mean-square error RMSE is equal to 0.0087, and the result indicates that the performance of the model is ideal.

Description

technical field [0001] The invention relates to a method for quickly quantifying the adulterated content of sodium bicarbonate in fresh milk, which belongs to the technical field of food detection. Background technique [0002] With the increase of raw milk production year by year, whether raw milk can be preserved in a standardized and effective manner has become a key issue in its quality control. Milk is rich in easily absorbed nutrients such as protein, energy, vitamins, amino acids and minerals, which also make it ideal for microbial growth and lead to the breakdown of lactose and the production of lactic acid, which increases the acidity of the milk. In order to cover up the rancidity of milk and reduce its acidity, sodium bicarbonate is often added to milk as an alkaline substance to neutralize the lactic acid in milk and prevent it from coagulating. Although sodium bicarbonate is often used as a bulking agent in the food industry, it is prohibited from being added t...

Claims

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Application Information

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IPC IPC(8): G16C20/20G06K9/62
CPCG16C20/20G06F18/214
Inventor 田怀香陈彬陈霜于海燕陈臣黄芝阳王晓雨
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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