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Method for promoting adventitious bud induction and cluster bud proliferation of curcuma zedoary

It is a technology of turmeric and bud induction, which is applied in the fields of botanical equipment and methods, horticultural methods, and plant regeneration to achieve the effects of promoting proliferation, robust plant growth, and promoting rooting.

Active Publication Date: 2022-01-04
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are few reports on the in vitro cultivation of plant tissues of Ezhu at home and abroad.

Method used

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  • Method for promoting adventitious bud induction and cluster bud proliferation of curcuma zedoary
  • Method for promoting adventitious bud induction and cluster bud proliferation of curcuma zedoary
  • Method for promoting adventitious bud induction and cluster bud proliferation of curcuma zedoary

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Experimental program
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Embodiment 1

[0013] The commercial name of the yucca extract of the present invention is Yucca Bo, which is produced by Mexico Agro-Industrial Corporation and imported and distributed by Shanghai Youjiu Biotechnology Co., Ltd. In the basal medium MS or 1 / 2MS of the present invention, the added amount of sucrose is 30 g / L, the added amount of agar is 7 g / L, and the pH needs to be adjusted to 5.8 before sterilization.

[0014] 1. Germination treatment

[0015] Excavate after the above-ground part of Ezhu is completely out of winter, select the rhizome material of Ezhu without rot and the same size, wash the cultivation soil, cut off the surface fibrous roots, clean the outer skin of the bulb, and dry it. 15 rhizomes per treatment. Soak the rhizomes of Zedoary Rhizome with different concentrations of yucca extracts (Table 1, the preparation method is to dissolve the yucca extracts in water) for 30 minutes, and then cover the bulbs with clean river sand and place them in a constant temperatur...

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Abstract

The invention discloses a method for promoting adventitious bud induction and cluster bud proliferation of curcuma zedoary. The method comprises the steps of accelerating germination of rhizome of curcuma zedoary, disinfection of explant bud points, induction of adventitious buds, proliferation of cluster buds and seedling strengthening and rooting. The method is characterized in that induction of the adventitious buds is that yucca extract is added into an induction culture medium. According to the method, the induced number of buds on the rhizome of the curcuma zedoary can be increased, induction of the adventitious buds can be promoted, the induced adventitious buds can be enlarged and thickened, plants can grow more robust, germ pollution is effectively reduced, proliferation of cluster buds is promoted, rooting is promoted, and the buds can be elongated and robust. Therefore, large-scale planting requirements can be met.

Description

Technical field: [0001] The invention belongs to the field of flower propagation, and in particular relates to a method for promoting the induction of adventitious buds and the multiplication of clustered buds. Background technique: [0002] Curcuma (C.elata) is a perennial herb widely distributed in Guangdong, Guangxi, Sichuan, and Yunnan, and its length can reach 80-150cm. The 4-7 leaves are basal, and the lower part of the sheath is often brownish purple. The leaves are generally oblong or oval, with a length of about 20-50 cm and a width of about 8-20 cm, with no hairs on the front and back of the leaves. The petiole is 1 / 3-1 / 2 the length of the blade. Curcuma curcuma has significant medicinal effects. Its ingredients can not only fight cancer and thrombus, eliminate accumulation and dissipate stagnation, but also regulate the stomach and intestines. Some scholars conducted a comprehensive evaluation of ornamental varieties of 10 Curcuma species with the help of analy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 盛爱武周丽颖冯欣张施君李艳兰石海桃
Owner ZHONGKAI UNIV OF AGRI & ENG
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