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Method for establishing acute plateau hypobaric and hypoxic myocardial injury animal model

An animal model and myocardial injury technology, which is applied in the field of biomedicine, can solve the problems of inaccurate parameter setting of low-pressure and hypoxic animal model cabin experimental conditions, acute HAMI animal model does not use multi-modal parameter verification, etc., to eliminate confounding interference factors. , the effect of stable altitude change rate

Active Publication Date: 2022-01-11
天津医科大学总医院空港医院
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Problems solved by technology

[0007] The experimental acute HAMI animal model is the basis for the study of hypobaric hypoxic myocardial injury, and the successful establishment of this model needs to improve two existing key problems: 1. Inaccurate setting of experimental condition parameters in the hypobaric hypoxic animal model cabin; 2. Acute HAMI Animal models not validated with multimodal parameters

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  • Method for establishing acute plateau hypobaric and hypoxic myocardial injury animal model
  • Method for establishing acute plateau hypobaric and hypoxic myocardial injury animal model
  • Method for establishing acute plateau hypobaric and hypoxic myocardial injury animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1. Experimental grouping and model building method condition setting

[0056] 1. Experimental grouping

[0057] (1) Preparation of experimental animals.

[0058] Purchase SPF grade 6-week-old, body weight 22.1g±0.7g, male Babl / c mice, and raise them in the laboratory environment for 7 days to adapt to the laboratory environment.

[0059] Experimental animal hypobaric hypoxia pre-experimental screening.

[0060] All experimental animals were placed in a low-pressure hypoxic animal model cabin, and the parameters in the cabin were set as follows: simulated altitude of 3500 meters; altitude change speed of 0.45m / s; pressure of 65.7KPa; oxygen concentration of 14.60%; temperature range of 16-28 ℃; humidity range 40-70RH%; noise <59dB, 3500 meters above sea level for 3 hours, observe and record the state of the experimental animals. During the experiment, the experimental animals with limbs stretched and no activity were defined as experimental animals with poo...

Embodiment 2

[0069] Embodiment 2. The process of raising experimental animals in the model cabin

[0070] (1) Control group: they were kept in a clean breeding room with a pressure of 101.3KPa and an oxygen content of 20.9% for 216 hours.

[0071] (2) 72h group: they were raised in a hypobaric hypoxic animal model cabin, and carried out according to the stage of ascending altitude, maintaining high altitude, and descending altitude.

[0072] (3) 144h group: they were raised in a hypobaric hypoxic animal model cabin, and carried out according to the stage of increasing altitude, the stage of maintaining high altitude in a cycle, and the stage of descending altitude.

[0073] (4) 216h group: they were raised in a hypobaric hypoxic animal model cabin, and carried out according to the stage of ascending altitude, cycle maintaining high altitude, and descending altitude.

[0074] (5) 216h continuous group: they were raised in a hypobaric hypoxic animal model cabin, and carried out according to...

Embodiment 118

[0135] Example 1. 18 F-FDG myocardial metabolic imaging experiment.

[0136] a: Prepare experimental animals. Take out the experimental animals of the experimental group, weigh them, and record the weight of the experimental animals. All experimental animals to be tested were fasted for 12 hours and placed in a 37°C incubator and transported to the Small Animal Imaging Center.

[0137] b: Preparation of myocardial metabolic imaging reagents. 18 F-FDG stock solution 1mCi, diluted to 100μL with 0.9%NaCl solution, draw 75μCi before each drug injection, diluted to 100μL with 0.9%NaCl 18 F-FDG working solution, tail vein injection, the reagents are ready-to-use.

[0138] c: Small animal PET / CT calibration. Turn on the small animal PET / CT, preheat the machine, click Auto Calibration, and confirm that the calibration is correct. Turn on the anesthesia machine, place the insulation blanket set at 37°C in the anesthesia induction box, adjust the concentration of isoflurane to 1.2...

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Abstract

The invention provides a method for establishing an acute plateau hypobaric and hypoxic myocardial injury animal model. The method comprises the following steps: firstly, setting three stages of altitude increasing, high altitude maintaining and altitude decreasing, simulating the altitude of 8500 meters, continuously operating for 216 hours, limiting the change rate of the simulated altitude to be 0.45 + / -0.04 m / s, enabling the air flow in a cabin to be stable, the air to be homogeneous and the oxygen content to be constant, and finally, obtaining the acute HAMI animal model. In addition, model-related characteristic change verification is carried out through 18F-FDG PET / CT myocardial metabolism imaging and small animal cardiac ultrasound in combination with FITC-BSA vascular leakage experiments, special staining of myocardial pathology and other means. According to the method for establishing the acute plateau hypobaric and hypoxic myocardial injury animal model, an optimization device and related specific parameter conditions required by modeling are defined, characteristic description is carried out on the model through a multi-mode combined verification means, and the animal model which is stable, reliable and capable of being continuously observed is provided for related research in the field.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for establishing an animal model of acute plateau hypobaric hypoxic myocardial injury. Background technique [0002] The main feature of the plateau environment is low pressure and hypoxia. The oxygen content at an altitude of 3,000 meters is equivalent to 75% of sea level. People living in low altitude areas who enter the plateau above 3,500 meters in an emergency may cause acute myocardial injury after a short period of exposure. [0003] The World Health Organization and the International Federation of Cardiology (WHO / ISFC) define acute high-altitude myocardial injury as a systemic disease and specifically as a specific cardiomyopathy. According to the time of exposure to hypobaric hypoxia, it can be divided into: acute hypobaric hypoxia, which means that people (tourists and climbers) who have been exposed to high altitude for hours or days become ill. Chronic hypobaric h...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2267/03
Inventor 张鹏李仕聪官宝礼张金霞宋世辉王欢
Owner 天津医科大学总医院空港医院
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