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POCT detection method for content of hypoxanthine in urine and application

A technology of hypoxanthine and detection method is applied in the field of POCT detection of hypoxanthine content in urine, and achieves the effects of facilitating popularization, speeding up diagnosis efficiency and saving detection time.

Pending Publication Date: 2022-01-11
AFFILIATED CHILDRENS HOSPITAL OF CAPITAL INST OF PEDIATRICS +1
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  • Abstract
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  • Claims
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Problems solved by technology

This method has been widely used in the detection of antigens and antibodies, but it is rarely used in the field of urine hypoxanthine content detection

Method used

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  • POCT detection method for content of hypoxanthine in urine and application
  • POCT detection method for content of hypoxanthine in urine and application
  • POCT detection method for content of hypoxanthine in urine and application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031] The invention establishes a portable simple POCT detection method for hypoxanthine content in urine, thereby providing a reference basis for the detection of epilepsy.

[0032] 1. The kit should be stored at 2-8°C. Kit specifications: 10 servings / box, see Table 1 for specific components

[0033] Table 1. Kit components

[0034]

[0035]

[0036] 2. Kit performance verification

[0037] 2.1 Sample collection

[0038] Enterprise standard products were selected for kit performance verification. All samples are stored at 2-8°C; if the samples are not used for a long time, they can be stored at -20°C.

[0039] 2.2 Sample response

[0040]Take 1mL of the standard product of each enterprise, add it to the reaction cup, blow it with a pipette for 5 times and mix well, let it stand at room temperature for 15 minutes, use a pipette to absorb 0.5mL of the fully reacted liquid in the previous step, and carefully drop it into the detection card. slot. Insert the detecti...

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Abstract

The invention belongs to the technical field of biological detection, and discloses a POCT (Point of Care Testing) detection method for the content of hypoxanthine in urine and application. The method effectively meets the requirement for clinical detection of the content of the hypoxanthine in the urine, and the content of the hypoxanthine in the urine can be rapidly, accurately and sensitively detected. The experiment result is good in repeatability and high in precision. The detection time period is short, the detection can be completed within 20min at the soonest, the detection time is saved, the efficiency is improved, and the method is an efficient auxiliary detection means. The method can be used for clinical and even household fast detection through POCT, and effectively makes up for the defects of clinical auxiliary epilepsy detection at present. An operator only needs to drop the collected urine into a reaction cup for mixed reaction, a reaction product is transferred to a detection card for on-machine detection, a detection instrument only depends on a common handheld spectrophotometer, a result can be automatically interpreted, the requirement for the operator is extremely simple, and clinical and household generalizability is high.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a POCT detection method and application of urine hypoxanthine content. Background technique [0002] Epilepsy, as one of the most common neurological diseases in the world, is one of the five major neuropsychiatric diseases that the World Health Organization (WHO) focuses on prevention and treatment. Epilepsy is the most common in childhood and seriously endangers children's physical and mental health. There are currently many clinical treatment options for children with epilepsy, including drug therapy, surgical treatment, neuromodulation, ketogenic diet therapy, and gene therapy under research. therapy and cell transplantation, etc. According to the patient's seizure type, combined with neuroimaging, neuroelectrophysiology, and specific biomarkers, an individualized diagnosis and treatment plan will bring more benefits to epilepsy patients. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/38
CPCG01N21/31G01N1/38
Inventor 陈倩毛莹莹张平平高彦彦冯硕姬辛娜谢丽娜肖江山于超计魏星
Owner AFFILIATED CHILDRENS HOSPITAL OF CAPITAL INST OF PEDIATRICS
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