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A kind of preparation method of small molecule β-1,3-glucan

A dextran and small molecule technology, applied in the field of small molecule β-1,3-glucan preparation, can solve the problems of harsh production conditions, large output of three wastes, pollution, etc., and achieve low production cost and simple steps , Environmentally friendly effect

Active Publication Date: 2022-03-22
JINAN GUOLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are problems such as complicated process and harsh production conditions in the preparation method of derivatives
The degradation preparation method, according to the existing, patent publications and literature introductions, mainly degrades β-1,3-glucan with a high degree of polymerization through physical, chemical, biological and other methods. There are complex production processes, low degradation efficiency, The composition of degradation products is complex, and physical and chemical methods still have problems such as large output of three wastes and serious pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of small molecule β-1,3-glucan, comprising the following steps:

[0028] S1. Activating the β-1,3-glucan-producing strain, inserting 500mL of seed medium for cultivation, and preparing a seed culture solution;

[0029] The β-1,3-glucan producing strain is Rhizobium GL-1803 (CGMCC No. 23416).

[0030] The composition of described seed culture medium: by mass percent, sucrose 2.0%, (NH 4 ) 2 HPO 4 0.5%, KH 2 PO 4 0.15%, MgSO 4 •7H 2 O 0.1%, CaCO 3 0.3%, corn syrup powder 0.15%, pH adjusted to 7.2.

[0031] The seed culture temperature is 30° C., and the shaking culture is 220 r / min for 24 hours to obtain a seed culture solution.

[0032]S2. Put the seed culture liquid into 6L fermentation medium to carry out fermentation culture.

[0033] The composition of described fermentation medium is: by mass percent, sucrose 5.0%, (NH 4 ) 2 HPO 4 0.2%, KH 2 PO 4 0.2%, MgSO 4 •7H 2 O 0.1%, CaCO 3 0.2%, corn syrup powder 0.2%, pH adjusted to...

Embodiment 2

[0053] Compared with Example 1, the difference is that after the fermentation in step S3, the fermentation broth is concentrated by ceramic membrane filtration until the solid content of macromolecular insoluble β-1,3-glucan is 40 g / L, and the other steps are all Same as Example 1. The purity of the obtained small molecule β-1,3-glucan product was 91.05%, and the total extraction yield was 83.65%.

Embodiment 3

[0055] Compared with Example 1, the difference is that after the fermentation in step S3, the fermentation broth is concentrated by ceramic membrane filtration until the solid content of macromolecular insoluble β-1,3-glucan is 60 g / L, and the other steps are all Same as Example 1. The purity of the obtained small molecule β-1,3-glucan product was 91.21%, and the total extraction yield was 82.34%.

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a preparation method of small molecule β-1,3-glucan. The method of the present invention uses the macromolecule insoluble β-1,3-glucan obtained from the fermentation of Rhizobium GL-1803 as a substrate, hydrolyzes it with endo-type β-1,3-glucanase, and obtains the purity by filtration and concentration ≥90% small molecule β-1,3-glucan; the preservation number of the Rhizobium GL-1803 is: CGMCC No.23416. The preparation method is simple, the production cost is low, and the environment is friendly, and the obtained small molecule β-1,3-glucan has high purity, which provides the possibility for large-scale production of the small molecule β-1,3-glucan.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of small molecule beta-1,3-glucan. Background technique [0002] β-1,3-glucan is a kind of polysaccharide whose main chain is connected by β-1,3-glycosidic bonds, and usually contains β-1,2-glycosidic bonds in different proportions and sizes due to different sources , β-1,4-glucosidic bonds, β-1,6-glycosidic bond-linked branched chains. β-1,3-glucan has biological activities such as enhancing immune activity, anti-tumor, anti-oxidation, anti-bacteria, anti-virus, anti-fungal, lowering cholesterol, lowering blood fat, etc., and is a good biological effect regulator. At the same time, it also has the functions of moisturizing, anti-inflammation, anti-aging, anti-wrinkle, anti-dandruff, anti-yellowing, accelerating repair, increasing skin elasticity, etc. It is widely used in many fields such as medicine, food, cosmetics, animal feed additives, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08C12P19/14C12N1/20C08B37/02C12R1/41
CPCC12P19/08C12P19/14C12N1/20C08B37/0024C08B37/0003
Inventor 袁建国黄魁吉武科
Owner JINAN GUOLI BIOLOGICAL SCI & TECH