PLGA nano particle for encapsulating DL-menthol and diclofenac as well as preparation method and application of PLGA nano particle
A diclofenac and nanoparticle technology, applied in the field of nanomaterials, can solve problems such as postoperative inflammation, tumor tissue residue, etc., and achieve the effects of reducing inflammatory response, good stability, and reducing adverse inflammatory response
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[0033] The present embodiment prepares the PLGA nanoparticle of encapsulating DL-menthol and diclofenac as follows:
[0034] (1) Dissolve 100mg PLGA powder and 50mg DL-menthol in 4mL CH 2 Cl 2 1 mL of DMSO solution containing 20 mg of diclofenac was added, and the resulting mixture was added to 20 mL of PVA solution with a mass concentration of 2%, and an emulsion was formed after probe ultrasonication (50% output power) for 5 min.
[0035] (2) The resulting emulsion was stirred at room temperature for 4 h. After the stirring, centrifuge at 9000rpm for 10min and wash with water 5 times to obtain PLGA nanoparticles encapsulating DL-menthol and diclofenac, which are denoted as diclofenac / DL-menthol@PLGA NPs (DC / DLM@PLGA NPs).
[0036] figure 2 It is the transmission electron microscope image of DC / DLM@PLGA NPs obtained in this example. It can be seen from the figure that the diameter of the nanoparticles is 340-460 nm.
[0037] image 3 It is the thermal phase transition ...
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