Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing xylooligosaccharide by utilizing electron beam irradiation and formic acid

A technology of electron beam irradiation and xylooligosaccharides, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve the problems of unsatisfactory production efficiency, difficulty in mass production, and high equipment requirements. Achieve high-efficiency preparation, high safety factor, and low equipment requirements

Pending Publication Date: 2022-01-28
HUNAN AGRICULTURAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional high-temperature self-hydrolysis and steam explosion methods are easy to directly hydrolyze the polymerized sugars in lignocellulose into monosaccharides, which is not ideal for production efficiency, and requires high temperature and high pressure, which consumes a lot of energy, which is not conducive to operation
Although the traditional acid-base method is simple and efficient, it requires a large amount of acid-base neutralization, which is likely to cause environmental pollution and requires high equipment
The reaction time of enzymatic hydrolysis is long, the efficiency is low, and it is difficult to carry out mass production in actual production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Carry out electron beam irradiation pretreatment to Nandi raw material, irradiation intensity is 500kGy, after crushing, pass through 40 mesh sieves, obtain sample.

[0033] (2) Accurately weigh 100 g of the sample in a glass reactor, and add a formic acid solution with a concentration of 78% according to a material-to-liquid ratio of 1:10 (m / v).

[0034] (3) Install the stirring blade and the condenser tube, and stir at a speed of 260 r / min for 2 hours under the condition of a water bath at 80°C.

[0035] (4) Suction filtration is performed after step (3) is finished, the filtrate is collected, and the filter residue is rinsed with 78% formic acid solution.

[0036] (5) The collected formic acid filtrate was placed in a round-bottomed flask, and the formic acid was collected by rotary evaporation in a water bath at 80° C. until there was no obvious liquid in the round-bottomed flask.

[0037] (6) Add a small amount of distilled water to the round bottom flask of s...

Embodiment 2

[0042] (1) Carry out electron beam irradiation pretreatment to Nandi raw material, irradiation intensity is 500kGy, after crushing, pass through 40 mesh sieves, obtain sample.

[0043] (2) Accurately weigh 100 g of the sample in a glass reactor, and add a formic acid solution with a concentration of 88% according to a material-to-liquid ratio of 1:10 (m / v).

[0044] (3) Install the stirring blade and the condenser, and stir for 2 hours at a speed of 260 r / min in a water bath at 70°C.

[0045] (4) Suction filtration is performed after step (3), the filtrate is collected, and the filter residue is rinsed with 88% formic acid solution.

[0046] (5) The collected formic acid filtrate was placed in a round-bottomed flask, and the formic acid was collected by rotary evaporation in a water bath at 80° C. until there was no obvious liquid in the round-bottomed flask.

[0047](6) Add a small amount of distilled water to the round bottom flask of step (5), and slightly ultrasonically v...

Embodiment 3

[0052] (1) Carry out electron beam irradiation pretreatment to Nandi raw material, irradiation intensity is 500kGy, after crushing, pass through 40 mesh sieves, obtain sample.

[0053] (2) Accurately weigh 100 g of the sample in a glass reactor, and add a formic acid solution with a concentration of 78% according to a material-to-liquid ratio of 1:10 (m / v).

[0054] (3) Install the stirring blade and the condenser, and stir for 3 hours at a speed of 260 r / min in a water bath at 90°C.

[0055] (4) Suction filtration is performed after step (3) is finished, the filtrate is collected, and the filter residue is rinsed with 78% formic acid solution.

[0056] (5) The collected formic acid filtrate was placed in a round-bottomed flask, and the formic acid was collected by rotary evaporation in a water bath at 80° C. until there was no obvious liquid in the round-bottomed flask.

[0057] (6) Add a small amount of distilled water to the round bottom flask of step (5), and slightly ult...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing xylooligosaccharide, which comprises the following steps: carrying out electron beam irradiation pretreatment on a plant raw material containing xylan, and crushing to obtain a sample; mixing the sample with a formic acid solution, and separating after the reaction is completed to obtain a first solution; evaporating and concentrating the first solution so as to remove formic acid and obtain a residual solid; mixing the residual solid with water to form a suspension, and separating to obtain a second solution; and deacidifying the second solution through an ion exchange resin. According to the method, the means of combining electron beam irradiation pretreatment and formic acid hydrolysis is adopted, xylooligosaccharide can be efficiently prepared, and the method is green and environmentally friendly.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a method for preparing xylooligosaccharides by using electron beam irradiation in cooperation with formic acid. Background technique [0002] Xylo-oligosaccharide is a component of xylan and is a functional oligosaccharide, which is widely used in the fields of agriculture, food, medicine and health products because of its unique physical and chemical properties and physiological functions. Generally, xylan-rich plant raw materials such as wheat straw, rice straw and reed can be prepared by different methods to prepare xylo-oligosaccharides. At present, common methods for preparing xylooligosaccharides mainly include high-temperature autohydrolysis, steam explosion, acid-base method, and enzymatic hydrolysis. [0003] The traditional high-temperature self-hydrolysis and steam explosion methods are easy to directly hydrolyze the polymerized sugars in lignocellulose into monosa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H1/00C07H1/06C07H3/06C07H3/02C08H7/00
CPCC07H1/00C07H1/06C07H3/06C07H3/02C08H6/00
Inventor 苏小军
Owner HUNAN AGRICULTURAL UNIV