Method for removing benzopyrene in flax extract

The technology of extract and benzopyrene is applied in the field of removal of benzopyrene in flax extract, and can solve the problems of uncontrollable loss of flax lignans, unsuitable removal of benzopyrene, high removal cost, etc. The effect of low cost, increased content and low removal cost

Pending Publication Date: 2022-01-28
西安天一生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The prior art cannot control the loss of the active ingredient flax lignans in the extract when removing benzopyrene, the cost of removal is high, the economic benefit is low, and it is not suitable for the removal of benzopyrene in plant extracts;
[0008] (2) The prior art does not have a method specifically for the removal of benzopyrene in plant extracts

Method used

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  • Method for removing benzopyrene in flax extract
  • Method for removing benzopyrene in flax extract
  • Method for removing benzopyrene in flax extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The method for removing benzopyrene in the flax extract provided by the embodiments of the present invention specifically includes:

[0053] (1) Ethanol extraction: take 200 g of linseed meal, extract with 75% ethanol, extract three times, 1.5 h each time, and the amount of extraction solvent is 8 / 6 / 6 times of the raw material. Combine the extracts, filter, concentrate the filtrate to no alcohol, and recover ethanol;

[0054] (2) Saponification: add lime water to the alcohol-free solution to adjust the pH to 11, react in a hot water bath, the hot water bath is 80 ° C, keep warm for 5 hours, and filter;

[0055] (3) acid adjustment: the filtrate is adjusted to pH 5 with oxalic acid, left to stand for 4 hours, precipitated, filtered, and collected;

[0056] (4) Alcohol dissolution: add 75% ethanol to dissolve the precipitate, the amount of ethanol added is 5 times of the precipitate, stir to dissolve completely.

[0057] (5) Column: PHD-100 non-polar resin is adsorbed o...

Embodiment 2

[0060] The method for removing benzopyrene in the flax extract provided by the embodiments of the present invention specifically includes:

[0061] (1) Ethanol extraction: take 500g of linseed meal, extract with 70% ethanol, extract three times, 1.5h / time, and the amount of extraction solvent is 10 / 8 / 6 times of the raw material. Combine the extracts, filter, concentrate the filtrate to no alcohol, and recover ethanol;

[0062] (2) Saponification: add lime water to the alcohol-free solution to adjust the pH to 11, react in a hot water bath, the hot water bath is 80 ° C, keep warm for 6 hours, and filter;

[0063] (3) acid adjustment: the filtrate is adjusted to pH 5 with oxalic acid, left to stand for 5 hours, precipitated, filtered, and collected;

[0064] (4) Alcohol dissolution: add 75% ethanol to dissolve the precipitate, the amount of ethanol added is 6 times of the precipitate, stir and dissolve completely.

[0065] (5) Putting on the column: LX-60 non-polar resin is ad...

Embodiment 3

[0068] The method for removing benzopyrene in the flax extract provided by the embodiments of the present invention specifically includes:

[0069] (1) Ethanol extraction: take 1000 g of linseed meal and extract with 65% ethanol for three times, 2 hours each time, and the amount of extraction solvent is 10 / 8 / 8 times of the raw material. Combine the extracts, filter, concentrate the filtrate to no alcohol, and recover ethanol;

[0070] (2) Saponification: add lime water to the alcohol-free solution to adjust the pH to 11, react in a hot water bath, the hot water bath is 85 ° C, keep warm for 4 hours, and filter;

[0071] (3) acid adjustment: the filtrate is adjusted to pH 5 with oxalic acid, left to stand for 4 hours, precipitated, filtered, and collected;

[0072] (4) Alcohol dissolution: add 75% ethanol to the precipitate to dissolve, the amount of ethanol added is 4 times of the amount of the precipitate, stir to dissolve completely.

[0073] (5) Putting on the column: D10...

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Abstract

The invention belongs to the technical field of plant component extraction, and discloses a method for removing benzopyrene in a flax extract, which comprises the following steps: extracting flaxseed meal after oil extraction with ethanol, mixing extracting solutions, filtering, concentrating until no alcohol exists, and recovering ethanol; adding lime water into the concentrated alcohol-free liquid to adjust the pH to be alkaline, performing a reaction in a hot bath, performing filtering after the reaction, and collecting filtrate; adjusting the pH value of the obtained filtrate to be acidic by using oxalic acid, standing, separating out a precipitate, filtering and collecting the precipitate; adding ethanol into the obtained precipitate for dissolving, and stirring until the precipitate is completely dissolved; and adsorbing by using non-polar resin, collecting effluent, and drying to obtain the flax extract. According to the invention, the content of benzo (a) pyrene is reduced to 0.5 ppb or below, the total amount of four polycyclic aromatic hydrocarbons is less than 2ppb and is far higher than the European Union standard, and the safety is high; meanwhile, the content of the effective component secoisolariciresinol diglucoside is increased, the yield is increased, the removal operation is simple, the cost is low, economy and effectiveness are realized, and industrial production can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant component extraction, in particular to a method for removing benzopyrene from flax extract. Background technique [0002] At present, flax, also known as flax, belongs to the dicotyledonous plants Rosaceae Linaceae annual herb, is one of the world's top ten oil crops. Flax meal is rich in lignans, the content of which is about 1% to 4%, which is 75 times higher than other foods containing lignans. The structure of flax lignans is very similar to that of human estrogen. It is considered to be a kind of phytoestrogen. It is a natural component produced by phenylalanine and cinnamyl alcohol dimerization reaction. It exists in the form of resinol diglucoside. Flax lignans have anti-oxidation, anti-tumor, anti-virus and fungi, anti-osteoporosis, lower serum cholesterol, enhance autoimmunity, delay aging, protect cardiovascular, inhibit aromatase activity, inhibit DNA and RNA synthesis, etc. In particu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/18
CPCC07H1/08C07H15/18
Inventor 杨景霞
Owner 西安天一生物技术股份有限公司
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