Collagenase-containing complex enzyme preparation and application thereof in treatment of leather leftovers

A compound enzyme preparation, collagenase-containing technology, applied in the direction of enzymes, peptidases, hydrolytic enzymes, etc., can solve the problems of wool fiber hydrolysis, low hydrolysis efficiency, and post-processing difficulties, achieving less addition, simple steps, and beneficial The effect of sustainable development

Pending Publication Date: 2022-01-28
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the leather inevitably contains wool fiber components. Conventional enzymatic methods cannot hydrolyze wool fibers, resulting in low hydrolysis efficiency, waste of wool fiber resources, and difficulties in post-processing. Enzyme preparation, effectively hydrolyzing waste scraps and obtaining valuable amino acid aqueous solution, reducing the amount of chemical reagents added has become one of the main technical research directions for those skilled in the art

Method used

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  • Collagenase-containing complex enzyme preparation and application thereof in treatment of leather leftovers
  • Collagenase-containing complex enzyme preparation and application thereof in treatment of leather leftovers
  • Collagenase-containing complex enzyme preparation and application thereof in treatment of leather leftovers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The compound enzyme preparation containing collagenase is composed of the following raw materials in unit mass parts:

[0038] Alkaline protease: alkaline lipase: keratinase: collagenase = 1.5: 1.5: 1.5: 2.5; wherein the parameters of each component are as follows:

[0039] The enzyme activity of alkaline protease is 700000U / g~800000U / g, the reaction temperature is 20℃~60℃, and the reaction pH is 8~12;

[0040] The enzyme activity of alkaline lipase is 1500U / g~2000U / g, the reaction temperature is 30℃~60℃, and the reaction pH is 7~10;

[0041] The enzyme activity of keratinase is 5000U / g~10000U / g, the reaction temperature is 25℃~60℃, and the reaction pH is 8~10;

[0042] The enzyme activity of collagenase is 1000U / g-1500U / g, the reaction temperature is 10°C-45°C, and the reaction pH is 8-10.

[0043] The application of the composite enzyme preparation containing collagenase in the treatment of leather scraps comprises the following process steps:

[0044] A. Put the l...

Embodiment 2

[0050] The compound enzyme preparation containing collagenase is composed of the following raw materials in unit mass parts:

[0051] Alkaline protease: alkaline lipase: keratinase: collagenase=0.5:0.5:0.5:1.5; wherein the parameters of each component are as follows:

[0052] Alkaline protease activity 700000U / g~800000U / g, reaction temperature 40℃~50℃, pH 10~11;

[0053] The enzyme activity of alkaline lipase is 1500U / g~2000U / g, the reaction temperature is 35℃~45℃, and the reaction pH is 7.5~8.5;

[0054] The enzyme activity of keratinase is 5000U / g~10000U / g, the reaction temperature is 35℃~45℃, and the reaction pH is 7.5~8.5;

[0055]The enzyme activity of collagenase is 1000U / g-1500U / g, the reaction temperature is 25°C-35°C, and the reaction pH is 7.5-8.5.

[0056] The application of the composite enzyme preparation containing collagenase in the treatment of leather scraps comprises the following process steps:

[0057] A. Put the leather solid waste in a 65°C water bath ...

Embodiment 3

[0063] The compound enzyme preparation containing collagenase is composed of the following raw materials in unit mass parts:

[0064] Alkaline protease: alkaline lipase: keratinase: collagenase = 1:1:1:2; the parameters of each component are as follows:

[0065] The enzyme activity of alkaline protease is 700000U / g~800000U / g, the reaction temperature is 20℃~60℃, and the reaction pH is 8~12;

[0066] The enzyme activity of alkaline lipase is 1500U / g~2000U / g, the reaction temperature is 30℃~60℃, and the reaction pH is 7~10;

[0067] The enzyme activity of keratinase is 5000U / g~10000U / g, the reaction temperature is 25℃~60℃, and the reaction pH is 8~10;

[0068] The enzyme activity of collagenase is 1000U / g-1500U / g, the reaction temperature is 10°C-45°C, and the reaction pH is 8-10.

[0069] The application of the composite enzyme preparation containing collagenase in the treatment of leather scraps comprises the following process steps:

[0070] A. Put the leather solid waste ...

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PUM

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Abstract

The invention relates to an enzyme preparation and application, in particular to a collagenase-containing complex enzyme preparation and application thereof in treatment of leather leftovers. The complex enzyme preparation comprises alkaline protease, alkaline lipase, keratinase and collagenase which are compounded in proportion, and the leather solid waste is subjected to hot bath, enzymolysis reaction and centrifugation to obtain hydrolysate. The technical problems that the technology is tedious, secondary pollution is serious, and the hydrolysis rate is low in the prior art are solved. The complex enzyme preparation has the advantages of simple process, high hydrolysis rate, no addition of chemical reagents, no secondary pollution and the like.

Description

technical field [0001] The invention relates to an enzyme preparation and its application, in particular to a compound enzyme preparation containing collagenase and its application in treating leather leftovers. Background technique [0002] my country's traditional tanning process has continued to this day. Leather processing requires dozens of processes and uses more than 100 chemical materials. The main purpose of the leather processing preparation stage is to remove tanning useless substances (such as hair, epidermis, fat, subcutaneous tissue, etc.) and loose collagen fibers in raw hides. This process will produce a large amount of tanning waste. The tanning industry only utilizes about 20% of the raw hide from raw hide to finished leather, and most of the leftovers from leather can only be piled up and buried, which brings great pressure on the environment and ecology. In 2020, the "Law of the People's Republic of China on the Prevention and Control of Environmental Po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/20C12P13/04
CPCC12N9/50C12N9/20C12P13/04C12Y301/01003C12Y304/24
Inventor 曹倩荣吴芳彤刘春卯郑翔秦梦王力源赵露秦艳梅马清河
Owner 河北省微生物研究所有限公司
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