A method of enzymatic treatment and extraction of chitin

A technology of chitin and quality, applied in the field of bioengineering, can solve the problems that have not yet been applied in large-scale industrial production, and achieve the effect of increasing hydrolysis rate, improving conversion rate, and mild separation conditions

Inactive Publication Date: 2019-07-19
邵方琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is still in the stage of laboratory research and has not yet been applied to industrial production on a large scale.

Method used

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  • A method of enzymatic treatment and extraction of chitin
  • A method of enzymatic treatment and extraction of chitin
  • A method of enzymatic treatment and extraction of chitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] After washing the crab shells with clean water, weigh 10g of crab shells and break them into pieces, each with an area less than 1cm 2 , Put it in a 100ml conical flask, add 20ml of 1mol / L hydrochloric acid, soak for 12 hours, filter to remove the liquid to obtain a solid substance. Pour the solid material back into the Erlenmeyer flask, add 20ml of fresh 1mol / L hydrochloric acid again, and soak for 12 hours until the crab shell becomes soft to remove the calcium component in the crab shell.

Embodiment 2

[0024] Filter the product obtained in Example (1), remove the liquid, pour the solid material back into the Erlenmeyer flask, add 20ml of deionized water, use 1mol / L hydrochloric acid or 1mol / L sodium hydroxide, carefully adjust the pH to about 3 About, add 100,000 U of acid protease (Zhengzhou Shiquanshimei Trading Co., Ltd. 100,000 U / g~300,000 U / g, or Zhengzhou Junkai Chemical Products Co., Ltd. 100,000 u / g) and substrate 1% by mass of Candida lipase was reacted in a shaker at 300 rpm and 40°C for 12 hours to remove protein components. At this time, the sample gradually changed from white to transparent, and a transparent product after hydrolysis was obtained.

Embodiment 3

[0026] Determination of product protein content after reaction:

[0027] Take 1 g of the transparent product obtained in Example 2 and use the BCA protein quantification kit (Tiangen Biochemical Technology (Beijing) Co., Ltd.) standard method to quantify the residual protein content in the product, and define the residual protein content as the protein content of the product after the reaction.

[0028] Determination of the protein content of the initial product:

[0029] After washing the crab shells with clean water, weigh 10g of crab shells and break them into pieces, each with an area less than 1cm 2 , Put it into a 100ml conical flask, add 20ml of 1mol / L hydrochloric acid, soak for 12 hours, filter to remove the liquid to obtain a solid. After taking a small amount of solids and adding 100,000 enzymatic (U) acid protease, take 1g of enzymatic hydrolysate, measure the protein content and define the measured protein content as the initial protein content.

[0030] The proteolysis e...

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Abstract

The invention provides a chitin production method which comprises the steps of (1) soaking shrimp and crab shells by 1-3mol / L hydrochloric acid, (2) adjusting pH (potential of hydrogen) of a soaking solution to be 2-4 and then adding acid proteinase and candida lipase for enzymolysis treatment, and (3) decolorizing the shrimp and crab shells subjected to the enzymolysis treatment, performing rinsing by water, and performing drying to obtain the chitin. According to the method, the chitin with high molecular weight is prepared by taking the shrimp and crab shells as raw materials and performing enzymatic reaction catalysis proteolysis. The method is simple to operate and mild in separation conditions, and greatly increases a conversion rate.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and particularly relates to a method for producing and separating chitin. Background technique [0002] Chitin is also known as chitin and chitin, and its active ingredient is chitosan (chitosan). In nature, chitin exists in the cells of lower plant fungi and algae, the shells of arthropod shrimp, crabs, insects, and the cell walls of higher plants. It is another important polysaccharide besides cellulose. Because the chemical structure of chitin is very similar to plant cellulose, chitin is also called animal fiber. The shell of arthropods contains 35% protein, 30% calcium and inorganic salts, and the rest is 35% chitin. [0003] In recent years, the role of chitin's biological functions in physiological health has been widely concerned, and its application potential is huge. In terms of regulating immunity, chitin can strengthen the proliferation of immune cells, so it has the effect of regu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/003
Inventor 邵方琦
Owner 邵方琦
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