Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of chemotactic factor CCL28 as marker in preparation of sicca syndrome diagnostic reagent

A technology of Sjogren's syndrome and diagnostic reagents, which is applied in the field of medical biological detection, can solve the problems of lack of clear indicators for the diagnosis of secondary Sjögren's syndrome, and achieve the effects of short cycle, sensitive detection, and easy clinical promotion

Pending Publication Date: 2022-01-28
CHONGQING TRADITIONAL CHINESE MEDICINE HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall diagnosis of secondary Sjogren's syndrome still lacks clear indicators

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of chemotactic factor CCL28 as marker in preparation of sicca syndrome diagnostic reagent
  • Application of chemotactic factor CCL28 as marker in preparation of sicca syndrome diagnostic reagent
  • Application of chemotactic factor CCL28 as marker in preparation of sicca syndrome diagnostic reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Serum CCL28 detects

[0036] This study included 60 patients with Sjogren's syndrome, 35 of which were primary Sjögren's syndrome and 25 patients with secondary Sjögren's syndrome (11 patients with Sjogren's syndrome secondary to rheumatoid arthritis, 14 called secondary to systemic lupus erythematosus). In addition, 18 subjects with rheumatoid arthritis, 10 subjects with systemic lupus erythematosus and 24 healthy controls were included in this study. The diagnosis of Sjögren’s syndrome was based on the international standards in 2002, the diagnosis of rheumatoid arthritis was based on the standards established by ACR and ELUAR in 2009, and the diagnosis of systemic lupus erythematosus was based on the classification standards of the International Lupus Research Clinical Cooperative Group (SLICC) in 2012.

[0037] During the study, blood was drawn from the patients, and the serum obtained after centrifugation was immediately frozen at -70°C.

[0038] 50...

Embodiment 2

[0046] Example 2: The present invention uses CCL28 as a serum marker for Sjogren's syndrome diagnostic reagent and its operation process

[0047] The diagnostic reagent of the present invention includes: CCL28 assay buffer, immunoadsorbent, enzyme-labeled conjugate, standard product and standard serum, washing liquid and stop liquid.

[0048] 60 patients diagnosed with Sjögren's syndrome (diagnostic criteria are the 2002 international classification diagnostic criteria) and 60 age- and gender-matched non-Sjögren's syndrome patients were detected by the ELISA method using the above diagnostic reagents, and the ROC for the diagnosis of Sjögren's syndrome was made. curve.

[0049] image 3 The ROC curve for the diagnosis of Sjogren's syndrome is CCL28 in serum as a diagnostic index.

[0050] The results indicated that the AUC value was 0.766, the Cutoff value was 945pg / ml, and the sensitivity of CCL28 in serum to diagnose Sjögren's syndrome was 70.49%, and the specificity was 7...

Embodiment 3

[0052] Example 3: Correlation between the degree of lip gland damage and serum CCL28 in different Sjogren's syndrome patients

[0053] Histological and immunohistochemical studies of the labial glands:

[0054] After obtaining the lip gland specimens of the patients, 4mm pathological sections of the lip gland specimens were made, and hematoxylin and eosin staining (H&E) was performed for routine histological examination. The lesion scoring method was used to judge the degree of lymphocyte infiltration of the specimen. Scoring was done by counting the number of focal inflammatory cell aggregates containing 50 or more monocytes per 4 mm2 area in salivary gland tissue.

[0055] After antigen retrieval, salivary gland paraffin sections were washed with 3% HO 2 o 2 Incubate for 10 min, add rabbit anti-human CCL28 antibody (Abcam, Cambridge, UK) diluted 1:100, and incubate overnight at 4°C. Goat anti-rabbit IgG antibody HRP polymer (Bioss, Beijing, China) was added to DAB counte...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a chemotactic factor CCL28 as a marker in preparation of a sicca syndrome diagnostic reagent. A detection sample of the diagnostic reagent is serum. According to the reagent, whether a subject suffers from the sicca syndrome or not and the degree of salivary gland injury of the subject suffering from the sicca syndrome are judged by monitoring the concentration of CCL28 in serum of the subject; and when the concentration of the CCL28 in the serum of the subject is lower than 900-990pg / ml, it is judged that the subject suffers from the sicca syndrome. The diagnostic reagent is easy and convenient to operate, reliable, high in sensitivity and specificity and capable of being used for clinical diagnosis and illness state evaluation.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to the application of chemokine CCL28 as a marker in the preparation of diagnostic reagents for Sjogren's syndrome. Background technique [0002] Sjogren's syndrome (SS) is a chronic inflammatory autoimmune disease mainly involving exocrine glands, also known as autoimmune exocrine gland epithelial cell inflammation or autoimmune exocrine disease. In addition to the decreased function of the salivary glands and lacrimal glands resulting in dry mouth and dry eyes, other exocrine glands and other organs outside the glands are also involved, resulting in multi-system damage symptoms. There are multiple autoantibodies and hyperimmunoglobulinemia in the serum. The disease is divided into primary and secondary categories. [0003] Primary Sjögren's syndrome is a global disease, with a prevalence rate of 0.3% to 0.7% in our country and 3% to 4% in the elderly populat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/58G01N33/53
CPCG01N33/6863G01N33/581G01N33/53G01N2333/521G01N2800/24
Inventor 李成荫龙海霞
Owner CHONGQING TRADITIONAL CHINESE MEDICINE HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com