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Application of diterpenoid compound

A compound and diterpenoid technology, applied in the application field of diterpenoids, can solve the problems of aggravation of the patient's condition, functional decline, drug failure, etc., and achieve a significant anti-islet cell apoptosis effect.

Active Publication Date: 2022-02-01
HAINAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although commercially available drugs can reduce the blood sugar level of T2DM patients, the apoptosis of pancreatic β cells leads to the decline of their function, which leads to the gradual aggravation of the patient's condition and the failure of the drugs
Therefore, in addition to good blood sugar control, the use of drugs that can reduce β-cell apoptosis is the new core of modern T2DM treatment. There is no anti-diabetic drug used in clinical practice that directly targets the apoptosis of pancreatic islet cells.

Method used

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  • Application of diterpenoid compound
  • Application of diterpenoid compound
  • Application of diterpenoid compound

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0027] The preparation of embodiment 1 compound of the present invention

[0028] Take 10 kg of wild licorice, extract with ethanol reflux, dissolve the extract in water after concentrating the extract, then extract with petroleum ether, recover the upper organic phase, and extract the remaining water layer with dichloromethane, recover the lower organic phase, and concentrate under reduced pressure , to obtain dichloromethane extract (180g).

[0029] Get the obtained dichloromethane extract, use silica gel dry method to mix the sample, add organic solvent to dissolve the dichloromethane extract, mix well with silica gel particles, after the organic solvent evaporates to dryness, grind finely, use silica gel column chromatography (silica gel 200 -300 mesh, particle size is 200-300μM), gradient elution is carried out with a mixed solution of petroleum ether and acetone, and the fraction obtained by using a volume ratio of petroleum ether and acetone of 100:16 is applied to a si...

Embodiment 2

[0035] Embodiment 2: Anti-apoptotic activity experiment of compounds of the present invention in MIN-6 cells

[0036] Methods: (1) Resurrection and culture of MIN6 cells: Take them out from the refrigerator at -80°C, centrifuge to discard the upper cryopreservation solution, add 1640 medium containing 10% fetal bovine serum and 1% double antibody to resuspend the cells, and the relative humidity is Culture in an incubator at 95%, 37°C, 5% CO2; depending on the growth of the cells, replace the fresh medium every 24-36 hours, and pass passage or freeze when the cells grow to 75%-85%. (2) Diterpenoid intervention model group (PA) culture: MIN6 cell proliferation grouping: well-grown MIN6 cells were evenly inoculated into 96-well plates, and divided into 5×10 5 100 μLC complete medium was added per mL. The cells were divided into the following groups: blank control group (CK), 300 μg / mL LC model group (PA), compound Ⅰ 25 μg / mL dosing group, 50 μg / mL dosing group. The original cu...

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Abstract

The invention belongs to the field of biochemical medicine, and discloses an application of a diterpenoid compound, the structure of the diterpenoid compound is shown in the specification, the diterpenoid compound is extracted and separated from wild liquorice, and cell experiments prove that the diterpenoid compound can remarkably resist islet cell apoptosis and has an important application prospect in treatment of T2DM.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of a diterpenoid compound. Background technique [0002] Type 2 diabetes mellitus (T2DM) has become a major health problem that seriously endangers human health and social and economic development. China is the country with the largest number of diabetic patients in the world, with a total of more than 100 million. The diabetes epidemic not only seriously endangers public health, but also imposes a huge economic burden on patients' families and society. The global economic burden of diabetes was about US$1.31 trillion in 2015, and it is predicted to increase to US$2.5 trillion per year by 2030. Insulin resistance is the root cause of the early development of T2DM. With the prolongation of the disease course, the number of pancreatic β cells gradually decreases, and their function also declines progressively. Pancreatic IsletβCells Apoptosis (Pancreatic Is...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P3/10C07C69/78C07C67/48C07C67/56
CPCA61K31/235A61P3/10C07C67/48C07C67/56C07C2602/28C07C69/78Y02A50/30
Inventor 董琳孙万莹张勇张小坡张彩云
Owner HAINAN MEDICAL COLLEGE