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Beta-human chorionic gonadotropin detection kit and production method thereof

A technology of chorionic gonadotropin and detection kit, which is applied in the field of immune detection, and can solve the problem that the detection effect of the beta human chorionic gonadotropin detection kit is not very satisfactory.

Pending Publication Date: 2022-02-01
NANJING LANSION BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The detection effect of the β-human chorionic gonadotropin detection kit of the above technical scheme is not very satisfactory. The reason is closely related to the selection and content of the reagent type

Method used

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  • Beta-human chorionic gonadotropin detection kit and production method thereof
  • Beta-human chorionic gonadotropin detection kit and production method thereof
  • Beta-human chorionic gonadotropin detection kit and production method thereof

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Embodiment Construction

[0029] Below in conjunction with the embodiment of the present invention further elaborates:

[0030] The β-human chorionic gonadotropin detection kit of this embodiment includes reagent R1, reagent R3 and reagent R4; wherein, the reagent R1 has streptavidin-coated magnetic particles and trihydroxymethylamino Methane Tris buffer, the reagent R3 has an acridinium ester-labeled mouse anti-human HCG antibody and phosphate buffer, and the reagent R4 has a biotin-labeled mouse anti-human HCG antibody and morpholineethanesulfonate MES;

[0031] Also included are calibrators and quality controls.

[0032] The reagent R1, the reagent R3 and the reagent R4 all contain the preservative Proclin 300.

[0033] The calibrators and quality controls are human chorionic gonadotropin recombinant antigen obtained through in vitro recombination in the state of lyophilized powder; the preservative Proclin 300 is 1 g / L.

[0034] In the reagent R1, the streptavidin-coated magnetic particle is 2.5g...

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Abstract

The invention discloses a beta-human chorionic gonadotropin detection kit. The beta-human chorionic gonadotropin detection kit comprises a reagent R1, a reagent R3 and a reagent R4, wherein the reagent R1 is provided with magnetic particles coated with streptavidin and a trihydroxymethyl aminomethane Tris buffer solution, the reagent R3 is provided with an acridinium ester labeled mouse anti-human HCG antibody and a phosphate buffer solution, and the reagent R4 is provided with a biotin labeled mouse anti-human HCG antibody and morpholine ethanesulfonic acid MES. The kit further comprises a calibration product and a quality control product. Buffer solutions are contained in the reagent R1, the reagent R3 and the reagent R4 (in the reagent R4, morpholine ethanesulfonic acid MES is the buffer solution), in addition, the beta-human chorionic gonadotropin detection kit comprises the calibration product and the quality control product, and the detection result is high in accuracy, good in anti-interference performance and good in stability.

Description

technical field [0001] The invention belongs to the field of immunoassay, and in particular relates to a beta-human chorionic gonadotropin detection kit and a preparation method thereof. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein with two non-covalently bound subunits. Its alpha subunit is similar to that of luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH). The beta subunit of human chorionic gonadotropin is distinct from other pituitary glycoprotein hormones and exhibits unique biochemical and immunological properties. Human chorionic gonadotropin is synthesized by placental cells and can maintain the corpus luteum function during pregnancy. It is a good pregnancy test marker and can be detected one week after conception. [0003] During pregnancy, human chorionic gonadotropin (hCG) levels rise exponentially and continue until approximately 8 to 10 weeks after the last menstrual per...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/58G01N33/577G01N33/543
CPCG01N33/76G01N33/577G01N33/583G01N33/54326G01N2333/59
Inventor 许行尚杰弗瑞·陈仲慧
Owner NANJING LANSION BIOTECH CO LTD
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