Scylla paramamosain antifungal polypeptide Spamptin72-91 and application thereof
A kind of anti-fungal technology, which is applied to anti-fungal agents, peptides/protein components, peptides, etc., can solve the problems of shortening the shelf life of food, changing color, affecting quality, etc., and achieve good antibacterial effect, short length, and antibacterial Active and stable effect
Active Publication Date: 2022-02-08
XIAMEN UNIV
View PDF8 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
When food is contaminated by mold, it will change color, become sticky and soft, decompose protein to produce sulfide and ammonia substances, cause spoilage, shorten the shelf life of food and affect the quality
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0028] A kind of pseudo cave blue crab novel antifungal polypeptide Spamptin of the present embodiment 72-91 The amino acid sequence of is SEQ ID NO: 01: Arg ThrAla Val Ser Leu Pro Arg Arg Pro Gln Phe Val Ile Lys Gln Met IleArg Ser.
[0029] In this example, Jill Biochemical (Shanghai) Co., Ltd. was entrusted to synthesize the antifungal polypeptide Spamptin of Scylla pseudocaveus with a purity of more than 95% by solid-phase synthesis. 72-91 , and provide detection information such as peptide molecular weight, HPLC, etc., and the relevant physical and chemical parameters are shown in Table 1 below.
[0030] Table 1 Antifungal peptide Spamptin 72-91 Physical and chemical parameters
[0031]
[0032] As can be seen from Table 1, the antifungal polypeptide Spamptin of Scylla pseudophylla of the present embodiment 72-91 Small molecular weight, good stability, strong water solubility, is a positively charged cationic polypeptide.
[0033] 1. Determination of Minimum Inhibit...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses a scylla paramamosain antifungal polypeptide Spamptin72-91 and the application thereof. The scylla paramamosain antifungal polypeptide Spamptin72-91 is composed of 20 amino acids, the molecular formula of the scylla paramamosain antifungal polypeptide Spamptin72-91 is C105H183N35O26S1, the molecular weight of the scylla paramamosain antifungal polypeptide Spamptin72-91 is 2383.89 Dalton, the scylla paramamosain antifungal polypeptide Spamptin72-91 contains 5 positively charged amino acid residues, the antifungal polypeptide Spamptin72-91 is predicted to have an isoelectric point of 12.48 according to charges of the amino acid residues, and the scylla paramamosain antifungal polypeptide Spamptin72-91 has good water solubility and is a positively charged cationic polypeptide. The compound has the characteristics of short length, easiness in synthesis, high antibacterial activity, stability and the like, and has a good application prospect in the fields of medicines, foods and the like.
Description
technical field [0001] The invention belongs to the technical field of marine molecular biology, and in particular relates to an antifungal polypeptide Spamptin 72-91 and its application. Background technique [0002] Fungi are widely distributed in nature, with various types and strong vitality. They can be spread by spores, and they mainly pollute food-related substances in daily production and life. When food is contaminated by mold, it will change color, become sticky and soft, decompose protein to produce sulfide and ammonia substances, cause spoilage, shorten the shelf life of food and affect the quality. In the process of food production, fungal contamination is likely to occur in every link from raw material production, storage, processing, transportation to use. Some fungi also produce toxic or carcinogenic secondary metabolites as they grow. Common toxin-producing fungi in daily life mainly include Aspergillus, Penicillium and Fusarium. The main toxins produced i...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/10
CPCC07K7/08A61P31/10A61K38/00
Inventor 王克坚张伟宾陈芳奕彭会熊明
Owner XIAMEN UNIV