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Method for catalytically synthesizing copolymer by using soybean seed coat peroxidase

A seed coat peroxidase and seed coat peroxide technology, applied in the field of polymer synthesis, can solve the problems of low conversion rate and low reaction efficiency, and achieve the effects of low cost, optimized process flow and controllable molecular weight

Pending Publication Date: 2022-02-08
NANJING ADVANCED BIOLOGICAL MATERIALS & PROCESS EQUIP INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: the present invention provides a method for catalyzing the synthesis of copolymers by using soybean seed coat peroxidase to solve the shortcomings of low reaction efficiency and low conversion rate in the prior art

Method used

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  • Method for catalytically synthesizing copolymer by using soybean seed coat peroxidase
  • Method for catalytically synthesizing copolymer by using soybean seed coat peroxidase
  • Method for catalytically synthesizing copolymer by using soybean seed coat peroxidase

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Provide monomer 1: chain transfer agent: catalyst: initiator: oxidant molar ratio is 120:1:0.00081:6:0.3, monomer 1: monomer 2 molar ratio=3:1, monomer 1 is DMA, monomer 2 for APEG 2300 . 12.26mg CTA1, 6.56g DMA, 39.25g TPEG 2300 and 250mL of PBS and DMSO mixed solution (PBS / DMSO=26:74v / v, [PBS]=20mM, pH=6) were added to the reaction flask and stirred evenly, and after adding 19.13mg soybean seed coat peroxidase and 0.33g ACAC Seal the bottle and place the reaction bottle in an ice-water bath to vent oxygen for 30 minutes, and then place it in an oil bath at 30°C. 2 o 2 The solution is injected into the reaction vial to initiate polymerization. After polymerizing for 2 h under a nitrogen atmosphere, the reaction flask was placed in an ice-water bath and oxygen was passed through to quench the reaction. Add a large amount of methanol to the reaction solution, stir and precipitate at low temperature for 4 hours, collect the precipitate by filtration, and put it in a ...

Embodiment 2

[0041] Let monomer 1: chain transfer agent: catalyst: initiator: oxidant molar ratio be 130:1:0.00081:6:0.3, monomer 1: monomer 2 molar ratio=4:1, monomer 1 is HEAA, monomer 2 for APEG 2300 . 12.26mg CTA1, 7.47g HEAA, 37.37g APEG 2300 and 200mL of PBS and DMSO mixed solution (PBS / DMSO=26:74v / v, [PBS]=20mM, pH=6) were added to the reaction flask and stirred evenly, and after adding 19.13mg soybean seed coat peroxidase and 0.56g BOP Seal the bottle and place the reaction bottle in an ice-water bath to vent oxygen for 30 minutes, and then place it in an oil bath at 30°C. 2 o 2 The solution is injected into the reaction vial to initiate polymerization. After polymerizing for 2 h under a nitrogen atmosphere, the reaction flask was placed in an ice-water bath and oxygen was passed through to quench the reaction. Add a large amount of methanol to the reaction solution, stir and precipitate at low temperature for 4 hours, collect the precipitate by filtration, and put it in a vac...

Embodiment 3

[0043] Let monomer 1:chain transfer agent:catalyst:initiator:oxidant molar ratio be 130:1:0.0006:6:0.3, monomer 1:monomer 2 molar ratio=3:1, monomer 1 is AA, monomer 2 for APEG 4000 . 12.26mg CTA2, 4.6g AA, 40.26g APEG 4000 and 250mL of PBS and DMSO mixed solution (PBS / DMSO=26:74v / v, [PBS]=20mM, pH=7) were added to the reaction flask and stirred evenly, and after adding 19.13mg soybean seed coat peroxidase and 0.33g ACAC Seal the bottle and place the reaction bottle in an ice-water bath to vent oxygen for 30 minutes, and then place it in an oil bath at 30°C. 2 o 2 The solution is injected into the reaction vial to initiate polymerization. After polymerizing for 2 h under a nitrogen atmosphere, the reaction flask was placed in an ice-water bath and oxygen was passed through to quench the reaction. Add a large amount of methanol to the reaction solution, stir and precipitate at low temperature for 4 hours, collect the precipitate by filtration, and put it into a vacuum dryi...

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Abstract

The invention discloses a method for catalytically synthesizing a copolymer by using soybean seed coat peroxidase, which comprises the following steps of: mixing a monomer 1, a monomer 2, a chain transfer agent, soybean seed coat peroxidase, an initiator and a solvent under the condition of inert gas atmosphere, preheating, then adding an oxidant under the condition of inert gas atmosphere, and reacting to obtain the copolymer. The monomer 1 is an acrylic acid or acrylamide compound, and the monomer 2 is a vinyl polyether macromonomer. Compared with traditional polymerization, the method has the advantages that the soybean seed coat peroxidase catalyst is used for carrying out an enzyme catalysis process, the advantages of greenness and high efficiency of bio-enzyme catalysis are combined, the reaction efficiency of inactive monomers (vinyl polyether macro-monomers) is greatly improved, the chain contents of different components in the product are close to theoretical values, the reaction time is shortened, and the reaction process has the advantages of safety, high efficiency, low energy consumption, high reaction rate, mild reaction conditions and the like.

Description

technical field [0001] The invention relates to polymer synthesis, in particular to a method for synthesizing a copolymer by catalyzing soybean seed coat peroxidase. Background technique [0002] Copolymers are also called interpolymers. A polymer obtained by polymerization of two or more different monomers. It can combine the excellent properties of various polymers to obtain functional polymer materials with superior performance. Enzyme is a biocatalyst with high efficiency and specificity. Enzyme catalysis has the characteristics of green, non-toxic, renewable, mild reaction conditions and environmental friendliness. At present, there are many methods for synthesizing copolymers. Enzymes catalyze RAFT polymerization through a one-pot method without intermediate purification steps to achieve one-step polymerization of monomers. However, there are two limitations in its preparation: 1) the reaction time is long and the reaction conditions are harsh; 2) it is difficult to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F283/06C08F220/54C08F220/58C08F2/38C12P7/40C12P7/62C12P13/02
CPCC08F283/065C12P13/02C12P7/62C12P7/40C08F220/54C08F220/58
Inventor 沈磊王慧悦李玉光季栋李亚军黄达刘一寰方正胡欣朱宁郭凯
Owner NANJING ADVANCED BIOLOGICAL MATERIALS & PROCESS EQUIP INST CO LTD