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Kit and method for simultaneously determining FT3 and FT4 in blood

A FT4 and kit technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection accuracy, narrow dynamic range, long detection time, etc., and achieve low quantitative lower limit, wide dynamic range, and easy operation. Effect

Active Publication Date: 2022-02-08
合肥歆智医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the large sample volume required for the detection of FT3 and FT4 in the prior art, the sample needs to be derivatized, the detection takes a long time, the detection accuracy is not high, and the operation is complicated, the specificity is low, the difference between batches is large, the sensitivity is low, and the dynamic range is low. Narrow technical problem, this application provides a kit for simultaneous determination of FT3 and FT4 in blood, including ultrafiltration device, buffer, FT3 and FT4 mixed calibrator, FT3 and FT4 mixed quality control, FT3 and FT4 mixed stable isotope Internal standard solution, this protocol is easy to operate, has high specificity, small batch-to-batch difference, high sensitivity, and wide dynamic range, which can meet the clinical requirements for FT3 and FT4 detection

Method used

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  • Kit and method for simultaneously determining FT3 and FT4 in blood
  • Kit and method for simultaneously determining FT3 and FT4 in blood
  • Kit and method for simultaneously determining FT3 and FT4 in blood

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A test kit for simultaneously measuring FT3 and FT4 in blood according to this embodiment, said test kit includes the following components:

[0040] Table 1 simultaneously measures the components of the test kit of FT3 and FT4 in blood

[0041]

[0042]

[0043] In this embodiment, the molecular weight cut-off of the ultrafiltration device of component 1 is 50K.

[0044] Component 2 buffer preparation includes: 12.570g of 4-hydroxyethylpiperazineethanesulfonic acid and 0.900g of sodium hydroxide, dissolved in 1L of purified water, shaken and ultrasonically mixed; take 15mL of the above buffer solution into a clean brown glass bottle, closed with parafilm.

[0045] Components 3 to 8 are FT3 and FT4 mixed calibrator with different concentrations, which are used to draw the calibration curve. Among them, the concentration of FT3 calibrator is 40, 20, 10, 4, 2, 1pg / mL, and the concentration of FT4 calibrator is 160, 80, 40, 16, 8, 4 pg / mL.

[0046] Components 9 and...

Embodiment 2

[0051] A kit for simultaneously measuring FT3 and FT4 in blood in this embodiment is basically the same as in Embodiment 1, except that sodium chloride, potassium dihydrogen phosphate, magnesium sulfate, urea, and calcium chloride are added to component 2 .

[0052] The specific preparation includes: 12.570g 4-hydroxyethylpiperazineethanesulfonic acid, 5.265g sodium chloride, 0.224g potassium dihydrogen phosphate, 0.275g magnesium sulfate heptahydrate, 0.300g urea, 0.275g calcium chloride dihydrate, dissolved Shake and ultrasonically mix in 1L of purified water; take 15mL of the above buffer solution into a clean brown glass bottle, and seal it with a parafilm.

[0053] In this embodiment, additional components are added to enhance the pH buffering capacity and ionic strength of the buffer, so that the parallelism of the ultrafiltration effect of different clinical serum samples is increased by 5-10% compared with Example 1, that is, different clinical serum samples are used i...

Embodiment 3

[0055] A kit for simultaneously measuring FT3 and FT4 in blood in this embodiment is basically the same as that in Embodiment 2, except that the molecular weight cut-off of the ultrafiltration device is 3K.

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Abstract

The invention belongs to the technical field of hormone detection, and relates to a kit and method for simultaneously determining FT3 and FT4 in blood. In the prior art, when FT3 and FT4 are detected, the volume of a required sample is large, the sample needs to be subjected to derivatization treatment, the detection time is long, the detection accuracy is not high, the operation is complicated, the specificity is low, the batch difference is large, the sensitivity is low and the dynamic range is narrow. Aiming to solve the above problems, the invention provides the kit for simultaneously determining FT3 and FT4 in blood. The kit comprises an ultrafiltration device, a buffer solution, an FT3 and FT4 mixed calibration product, an FT3 and FT4 mixed quality control product and an FT3 and FT4 mixed stable isotope internal standard solution. The kit is simple and convenient to operate, high in specificity, small in batch difference, high in sensitivity and wide in dynamic range, and can meet clinical FT3 and FT4 detection. The invention further provides a method for simultaneously determining FT3 and FT4 in blood, derivatization treatment is not needed, and the detection steps are simplified.

Description

technical field [0001] The invention belongs to the technical field of hormone detection, and in particular relates to a kit and method for simultaneously measuring FT3 and FT4 in blood. Background technique [0002] The thyroid gland is an endocrine gland that secretes thyroid hormones and exerts extensive and strong physiological effects on the body. Triiodothyronine (T3) and thyroxine (T4) are two main thyroid hormones, which can promote individual growth and development, increase the basic metabolic rate of the body, improve the oxidative decomposition of sugar, fat, and protein, and increase In addition, thyroid hormones also have important physiological effects on the central nervous system, cardiovascular system, and digestive system. About 10% of T3 and T4 in the blood come from the thyroid gland, and the remaining about 90% of T3 is produced by the deiodination metabolism of T4 in peripheral tissues. In the blood circulation, T3 and T4 exist in two forms: bound st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 彭军江振作陈文慧刘超谢沙莎
Owner 合肥歆智医疗器械有限公司