Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Low-phenylalanine vegetable protein peptide and preparation method thereof

A plant protein peptide and low-phenylalanine technology, applied in the field of low-phenylalanine plant protein peptide and its preparation, can solve the problems of less development and utilization of pea protein, improve the utilization rate of adsorbent, reduce the dosage, and facilitate absorption effect

Pending Publication Date: 2022-02-11
CHINA AGRI UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pea protein is currently less developed and utilized, and has a lot of room for development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-phenylalanine vegetable protein peptide and preparation method thereof
  • Low-phenylalanine vegetable protein peptide and preparation method thereof
  • Low-phenylalanine vegetable protein peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Weigh a certain amount of pea protein powder, add distilled water to make a solution, pretreat in a 90°C water bath for 15 minutes, cool, and then heat the solution to 55°C on a constant temperature magnetic stirrer, add water, 15 mol / L disodium hydrogen phosphate- Potassium dihydrogen phosphate buffer and NaOH were used to adjust the pH to 8.4, the protein concentration was 3.0%, and the enzyme was added in an amount of 3600 U / g to add alkaline protease and mix well, and the hydrolysis was carried out at a constant temperature of 55°C for 3 hours. After the alkaline protease hydrolysis is completed, inactivate the alkaline protease in a boiling water bath for 10 minutes, and cool to room temperature. Then add 4000U / g flavor protease and hydrolyze for 3h under the conditions of 55°C and pH7.5.

[0043] Phenylalanine was removed by activated carbon adsorption. After the enzymatic hydrolysis, the solution was centrifuged at 6000r / min for 20 min, the supernatant was collect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a low-phenylalanine vegetable protein peptide and a preparation method thereof, and belongs to the field of food. The content of phenylalanine in the low-phenylalanine plant protein peptide accounts for 0.13-0.19% of the protein equivalent, and the low-phenylalanine plant protein peptide has the potential of being used for preparing formula food or health care products with special medical purposes for patients suffering from phenylketonuria and the like. The method comprises the following steps: dissolving plant protein powder, carrying out two-step protease enzymolysis on the plant protein powder by using alkaline protease and flavourzyme, and centrifuging the obtained zymolyte to remove insoluble substances; enabling the collected supernate to be subjected to static adsorption by activated carbon to remove phenylalanine, and obtaining a low-phenylalanine vegetable protein peptide mixed solution; carrying out vacuum filtration to remove the activated carbon, and performing freeze-drying to obtain the low-phenylalanine vegetable protein peptide powder. The vegetable protein is hydrolyzed through a compound enzyme method, more than 96% of phenylalanine in the vegetable protein can be removed, and the preparation method of the low-phenylalanine protein peptide is effective.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a low-phenylalanine vegetable protein peptide and a preparation method thereof. Background technique [0002] Phenylketonuria is an amino acid metabolic disease caused by phenylalanine hydroxylase dysfunction or dihydrobiosynthetic enzyme / dihydrobiopterin reductase deficiency in the phenylalanine metabolic pathway in patients, which can cause Various severe symptoms. The global incidence of phenylketonuria is about 1 / 15,000, and the incidence in China is about 1 / 11,000. If the neonatal patients with phenylketonuria do not take measures in time, they will have symptoms such as mental retardation, psychoneurological symptoms, eczema, skin scratches, depigmentation, and rat odor. In severe cases, they may suffer from cerebral palsy. At present, controlling the intake of phenylalanine in patients' daily diet is still the safest and most effective therapeutic intervention for phenylk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 车会莲刘桂蓉朱皓茹曾冰蕙李博雅熊文文陈成韩诗雯
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com