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Preparation process development of special polyglutamic acid biostimulant for traditional Chinese medicine planting

A technology of biostimulants and polyglutamic acid, which is applied in the fields of chemicals for biological control, botany equipment and methods, and plant growth regulators, and can solve problems such as poor growth status, high viscosity, and yield decline

Inactive Publication Date: 2022-02-15
JINAN HANGCHEN BIOTECHNOLOGY CO LTD +4
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the relative average molecular weight of polyglutamic acid can be as high as 1000KDa or more, and the viscosity is high, when it acts on crops as a synergist, if the concentration is improper, it is easy to wrap the seeds or roots, resulting in the contact area between the seeds or roots and the air. decrease, which is detrimental to the growth of crops
[0004] Most of the traditional Chinese medicine planting areas, especially the areas where the traditional Chinese medicine is planted all the year round, will have the problem of continuous cropping obstacles, resulting in reduced yield, poor quality, and poor growth status, especially for traditional medicines that use roots or rhizomes. Continuous cropping obstacles are prone to occur when plants are used for continuous cropping

Method used

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  • Preparation process development of special polyglutamic acid biostimulant for traditional Chinese medicine planting

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation process development of a special polyglutamic acid biostimulant for Chinese medicine planting of the present invention comprises the following steps:

[0019] 1) Seed liquid medium formula ( / L): glucose 10g, yeast powder 6g, ammonium chloride 3g, sodium glutamate 5g, magnesium sulfate 0.5g, pH7.3;

[0020] 2) Fermentation medium formula ( / L): 40g sucrose, 10g peptone, 10g ammonium sulfate, 20g sodium glutamate, 0.6g magnesium sulfate, 2g dipotassium hydrogen phosphate, pH 7.4;

[0021] 3) Inoculate Bacillus licheniformis into a conical flask with seed medium, culture at 37°C, shaker speed 180r / min, and culture for 16 hours to obtain seed liquid;

[0022] 4) Add 0.3% hydrochloric acid solution to the above seed solution, inoculate it in the fermentation medium with an inoculum size of 10%, and culture it with aeration and stirring at 37° C. for 24 hours to prepare a fermentation broth of 10KDa-18KDa low molecular weight polyglutamic acid;

[0023] 5) 10 ...

Embodiment 2

[0026] 1) Seed liquid medium formula ( / L): glucose 10g, yeast powder 6g, ammonium chloride 3g, sodium glutamate 5g, magnesium sulfate 0.5g, pH7.3;

[0027] 2) Fermentation medium formula ( / L): 40g sucrose, 10g peptone, 10g ammonium sulfate, 20g sodium glutamate, 0.6g magnesium sulfate, 2g dipotassium hydrogen phosphate, pH 7.4;

[0028] 3) Inoculate Bacillus licheniformis into a conical flask with seed medium, culture at 37°C, shaker speed 180r / min, and culture for 16 hours to obtain seed liquid;

[0029] 4) Take the above seed liquid and add 0.03% hydrochloric acid solution, inoculate it in the fermentation medium, the inoculum size is 10%, cultivate it with aeration and stirring at 37°C for 24 hours, and prepare the fermentation liquid of 20KDa-40KDa low molecular weight polyglutamic acid;

[0030] 5) 10 parts by weight of 1KDa-2KDa low molecular weight polyglutamic acid fermentation liquid, 10 parts of potassium humate, 7 parts of seaweed extract, 5 parts of potassium dihyd...

Embodiment 3

[0033] 1) Seed liquid medium formula ( / L): glucose 10g, yeast powder 6g, ammonium chloride 3g, sodium glutamate 5g, magnesium sulfate 0.5g, pH7.3;

[0034] 2) Fermentation medium formula ( / L): 40g sucrose, 10g peptone, 10g ammonium sulfate, 20g sodium glutamate, 0.6g magnesium sulfate, 2g dipotassium hydrogen phosphate, pH 7.4;

[0035] 3) Inoculate Bacillus licheniformis into a conical flask with seed medium, culture at 37°C, shaker speed 180r / min, and culture for 16 hours to obtain seed liquid;

[0036] 4) Take the above seed liquid and add 0.003% hydrochloric acid solution, inoculate it in the fermentation medium, the inoculum size is 10%, cultivate it with aeration and stirring at 37°C for 24 hours, and prepare the fermentation liquid of 60KDa-100KDa low molecular weight polyglutamic acid;

[0037] 5) 10 parts by weight of 1KDa-2KDa low molecular weight polyglutamic acid fermentation liquid, 10 parts of potassium humate, 7 parts of seaweed extract, 5 parts of potassium dih...

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Abstract

The invention discloses development of a preparation process of a special polyglutamic acid biostimulant for traditional Chinese medicine planting. The biostimulant is composed of bacillus licheniformis, low-molecular polyglutamic acid generated by fermentation, added potassium humate, a seaweed extracting solution, urea, monopotassium phosphate, calcium hydrophosphate, ammonium bicarbonate, magnesium sulfate, ferrous sulfate, zinc sulfate and manganese sulfate. The special polyglutamic acid biostimulant for traditional Chinese medicine planting can fully stimulate the intrinsic potential of traditional Chinese medicine plants; meanwhile, low-molecular-weight polyglutamic acid and chelated medium trace elements are added, the slow release property is achieved, the utilization rate of a fertilizer can be increased, growth of the traditional Chinese medicine plants is promoted, the yield of traditional Chinese medicines is increased, cost is saved, and meanwhile, environmental pollution is reduced.

Description

Technical field [0001] The invention belongs to the field of fermentation fertilizers, and specifically relates to the development of a preparation process of polyglutamic acid biostimulant specially used for traditional Chinese medicine planting. Background technique [0002] Biostimulants are a general term for various substances and microorganisms that can promote plant growth. They are mainly divided into 8 categories: humic acid, complex organic materials, beneficial chemical elements, inorganic salts (including phosphite esters), seaweed extracts, crustaceans Vitamins and chitosan derivatives, anti-transpiration agents, free amino acids and other nitrogen-containing substances. Biostimulants are not traditional fertilizers. During the growth process of crops, cell division, chlorophyll conversion, cell wall formation, crop stress resistance, that is, immunity, and other physiological behaviors are different in different regions, soils, and climates. It even has a big ...

Claims

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Application Information

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IPC IPC(8): C05G3/80A01N65/03A01N61/00A01N37/46A01P21/00
CPCC05G3/80C05B3/00A01N65/00A01N65/03A01N61/00A01N37/46C05B7/00C05C3/00C05C9/00C05C11/00C05D5/00C05D9/02C05F11/00C05F11/02C05F11/10
Inventor 赵林李昆仑李宝君赵晨岳秋林苏乐孙欣田丽
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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