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Bacterium loaded with nano drug-loaded particles, preparation method and application thereof

A nano-drug and bacteria technology, applied in the field of nano-medicine, can solve the problems of reducing the effectiveness of bacterial delivery, increasing toxicity, and falling off nanoparticles, and achieving the effects of rapid bioorthogonal reaction, reducing toxicity and increasing effectiveness.

Pending Publication Date: 2022-02-18
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, one method of loading nanoparticles on the surface of bacteria is the conjugation of chemical groups, which easily destroys the active sites on the surface of bacteria and affects their recognition and function; the other is adsorption by physical means such as positive and negative charges. , this method is not stable enough, it is easy to cause the shedding of nanoparticles in the blood circulation, reduce the effectiveness of bacterial delivery, and increase the toxicity

Method used

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  • Bacterium loaded with nano drug-loaded particles, preparation method and application thereof
  • Bacterium loaded with nano drug-loaded particles, preparation method and application thereof
  • Bacterium loaded with nano drug-loaded particles, preparation method and application thereof

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Embodiment 1 4

[0060] The modification of the azide monosaccharide analogue of embodiment 1 tetraacetylation to the surface of anaerobic bacteria Salmonella

[0061] Carbohydrate derivatives carrying azide (Ac 4 ManNAz, Ac 4 GalNAz, Ac 4 GlcNAz) was added into simple DMEM medium, and the medium with a final concentration of 50 μg / mL was prepared. Cultivate Salmonella (YB1) in the prepared DMEM medium for 48-72 hours under anaerobic conditions, and use the endogenous sugar metabolism process of the bacteria to embed the azide group on the surface of the YB1 bacterial membrane to prepare azide-modified YB1 bacteria (N 3 -YB1).

Embodiment 2

[0062] The modification of the azide monosaccharide analogue of embodiment 2 tetraacetylation to the surface of anaerobic bacteria Salmonella

[0063] Carbohydrate derivatives carrying azide (Ac 4 ManNAz, Ac 4 GalNAz, Ac 4 GlcNAz) was added to simple DMEM medium to prepare a medium with a final concentration of 100 μg / mL. Cultivate Salmonella (YB1) in the prepared DMEM medium for 48-72 hours under anaerobic conditions, and use the endogenous sugar metabolism process of the bacteria to embed the azide group on the surface of the YB1 bacterial membrane to prepare azide-modified YB1 bacteria (N 3 -YB1).

Embodiment 3

[0064] Example 3 Modification of tetraacetylated azide monosaccharide analogs on the surface of magnetobacteria

[0065] Carbohydrate derivatives carrying azide (Ac 4 ManNAz, Ac 4 GalNAz, Ac 4 GlcNAz) was added to pure DMEM medium to prepare a medium with a final concentration of 150 μg / mL. Cultivate Salmonella (YB1) in the prepared DMEM medium for 48-72 hours under anaerobic conditions, and use the endogenous sugar metabolism process of bacteria to embed azide groups on the surface of Salmonella (YB1) membrane to prepare azide Modified YB1 bacteria (N 3 -YB1).

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Abstract

The invention discloses bacterium loaded with nano drug-loaded particles and a preparation method and application of the bacterium, the surface of a photosynthetic bacterium or anaerobic bacterium is modified with a first click chemical group in a cell metabolism mode, and a second click chemical group is conjugated to the nano drug-loaded particles; and finally, loading of the nano drug-loaded particles on the surfaces of the bacterium is realized through a click chemistry mediated biological orthogonal reaction. The method does not influence the function and recognition mode of the bacterium, meanwhile, the biological orthogonal reaction is non-toxic and rapid, the specific chemical linking method can efficiently load anti-cancer nano drug-loaded particles on the surface of the bacterium, and effective carrying of the nano particles by the bacterium is truly achieved; and the bacterium loaded with nano drug-loaded particles prepared by the method can be applied to the development of antitumor drugs.

Description

technical field [0001] The invention relates to the field of nanomedicine, in particular to a bacterium loaded with nanometer drug-loaded particles and its preparation method and application. Background technique [0002] In the past 20 years, cancer in my country has shown a trend of younger age and higher morbidity and mortality in the "third line". According to the average life expectancy of 74 years, the probability of suffering from malignant tumors in one's life is 22%. Tumors have become a common disease. How to effectively prevent and treat cancer has become a top priority in scientific research. The rapid development of new technologies has had a huge impact on the progress of tumor medicine, and nano-biomedicine is a new discipline developed with the development of nano-technology. Nanobiomedicine has gained more and more recognition in the diagnosis and treatment of major diseases such as cancer, cardiovascular, neurology, and metabolism. The structure of nanoma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K45/00A61P35/00B82Y5/00B82Y40/00C12N1/20C12R1/42
CPCA61K47/6901A61K47/6931A61K45/00A61P35/00B82Y5/00B82Y40/00C12N1/20C12N1/005
Inventor 蔡林涛李文军梁振国臧中盛刘陈立贺伟邓冠军
Owner SHENZHEN INST OF ADVANCED TECH