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Immunoregulation-based stem cell membrane camouflage nano-drug as well as preparation method and application thereof

A technology of nano-medicine and immune regulation, which is applied in stem cell membrane-disguised nano-medicine to treat bone diseases. In the field of preparation of nano-medicine, it can solve the problem of repairing bone damage and less disease, and achieve the effect of improving the pathological microenvironment

Pending Publication Date: 2022-03-01
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the induction of immunological processes including magnetic polarization, magnetothermal, magnetic force and other magnetic effects to repair bone damage diseases

Method used

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  • Immunoregulation-based stem cell membrane camouflage nano-drug as well as preparation method and application thereof
  • Immunoregulation-based stem cell membrane camouflage nano-drug as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Adipose-derived mesenchymal stem cell membrane camouflaged ferric oxide and denosumab nanomedicine

[0026] Adipose-derived mesenchymal stem cell membrane camouflaged nano-medicine, including adipose-derived mesenchymal stem cell membrane, iron ferric oxide nanoparticles (size 10-15nm) and denosumab (anti-osteoporosis drug) three components, nano The size of the drug is 100-200nm, the drug loading capacity is 6-18 mg / mL of iron, and 5-20 mg / mL of denosumab.

[0027] In the preparation process, firstly, adipose-derived mesenchymal stem cells are extracted by hypotonicity, homogenization and ultrasonic treatment to prepare a uniform stem cell membrane; at the same time, chemical co-precipitation method is used to prepare ferric oxide nanoparticles with good water dispersibility; secondly, using Ferric oxide nanoparticles and denosumab were co-loaded into the stem cell membrane by sonoporation, and the stem cell membrane disguised nano-drug with uniform size was...

Embodiment 2

[0030] Example 2. Bone marrow-derived mesenchymal stem cell membrane camouflaged manganese dioxide and strontium ranelate nanomedicine

[0031] Bone marrow-derived mesenchymal stem cell membrane camouflaged nanomedicine, including three components: bone marrow-derived mesenchymal stem cell membrane, manganese dioxide nanosheets (radial size 50-85nm) and strontium ranelate (anti-osteoporosis drug), The size of the nano drug is 200-400nm. The drug loading is 10-25 mg / mL of manganese and 50-70 mg / mL of strontium ranelate.

[0032] The preparation process of the bone marrow-derived mesenchymal stem cell membrane is similar to the preparation process of the adipose-derived mesenchymal stem cell membrane in Example 1; at the same time, a hydrothermal method is used to prepare manganese dioxide nanosheets with good water dispersibility; Manganese nanosheets and strontium ranelate were co-loaded into the stem cell membrane, and the stem cell membrane with uniform size was obtained by...

Embodiment 3

[0034] Example 3. Umbilical cord blood-derived mesenchymal stem cell membrane disguised zinc ferrite and hydroxyapatite nano-medicine

[0035] Umbilical cord blood-derived mesenchymal stem cell membrane camouflage nano-medicine, including cord blood-derived mesenchymal stem cell membrane, zinc ferrite nanoparticles (size 100-150nm) and hydroxyapatite (drug for repairing bone damage, size 80-20nm) A component, the size of the nano drug is 500-800nm. The drug loading is 20-50 mg / mL of iron and 10-20 mg / mL of phosphorus.

[0036] The preparation process of umbilical cord blood-derived mesenchymal stem cell membrane is similar to the preparation process of adipose-derived mesenchymal stem cell membrane in Example 1; at the same time, zinc ferrite nanoparticles and hydroxyapatite nanoparticles with good water dispersibility are prepared respectively by hydrothermal method Secondly, the zinc ferrite nanoparticles and denosumab were co-loaded into the stem cell membrane by magnetic ...

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Abstract

The invention discloses a stem cell membrane camouflage nano-drug based on immunoregulation as well as a preparation method and application of the stem cell membrane camouflage nano-drug. The nano-drug is formed by loading a magnetic nano-material and a bone disease treatment drug on a stem cell membrane, the stem cell membrane camouflage nano-drug can break through various physiological barriers through an immune escape and membrane lubrication interface to reach a bone focus area, and can reside for a long time through mutual fusion with local cells and tissues. A magnetic effect is generated through stimulation of an external magnetic field device, long-term immunoregulation treatment of a bone injury area is jointly achieved in combination with slow release of drugs, and great clinical development potential is achieved.

Description

technical field [0001] The invention relates to the field of drugs for the diagnosis and treatment of bone-related diseases, in particular to stem cell membrane camouflage nano-medicine based on immune regulation, and also relates to a preparation method and application of the nano-medicine in the treatment of bone diseases. Background technique [0002] Refractory bone diseases represented by osteoarthritis, osteoporosis, and cartilage damage, involving cartilage degradation, subchondral bone sclerosis, and osteophyte formation, are characterized by inflammation, cartilage loss, and bone changes. Chronic degenerative disease. It is estimated that 15% of people over the age of 60 in the world currently suffer from clinical symptoms of cartilage damage. Due to the scarcity of blood vessels, the damaged cartilage is difficult to repair itself, which manifests as a degenerative and inflammatory process, which promotes the progression of the disease. However, there are still ma...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/381A61K38/18A61K41/00A61K45/00A61K47/46A61K47/02A61P19/02A61P19/08A61P19/10
CPCA61K9/5176A61K9/5115A61K45/00A61K41/00A61K31/381A61K38/1825A61P19/02A61P19/10A61P19/08
Inventor 李长明陈博史转转邹卓孙会萍袁颖赵轩宇
Owner SUZHOU UNIV OF SCI & TECH