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Method for preparing kaempferol and afzelin from Chinese redbud leaf extract

A technology for alfodzin and extract is applied in the field of preparing kaempferol and alfondoside, which can solve the problems of low extraction rate and the like, and achieve the effects of high extraction rate, simple extraction process and large extraction amount.

Pending Publication Date: 2022-03-01
HENAN UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of kaempferol and afudouside in Bauhinia leaves is relatively high, but there is no report of extracting kaempferol from Bauhinia plants in large quantities. In the existing method for extracting afudouside from Bauhinia leaves (CN 201710206992.7), it is necessary to Using silica gel column chromatography will produce silica gel waste and the extraction rate is low

Method used

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  • Method for preparing kaempferol and afzelin from Chinese redbud leaf extract

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for preparing kaempferol and arfudoside from Bauhinia leaf extract, comprising the steps of:

[0029] (1) Take dried Bauhinia leaves as raw materials, grind them and extract them with 60% ethanol at 40°C, and extract twice for 1 hour each time;

[0030] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0031] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0032] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to NKA-9 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0033] (5) The 30% ethanol eluted part was concentrated under reduced pressure, dissolved in methanol, passed through a ...

Embodiment 2

[0036] A method for preparing kaempferol and arfudoside from Bauhinia leaf extract, comprising the steps of:

[0037] (1) Take 1 kg of dried Bauhinia leaves as raw material, grind and extract with 85% ethanol at 50°C, extract 3 times, each time for 1.5h;

[0038] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0039] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0040] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to NKA-9 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0041] (5) The 30% ethanol eluted part was concentrated under reduced pressure, dissolved in methanol, passed through a Sephad...

Embodiment 3

[0049] A method for preparing kaempferol and arfudoside from Bauhinia leaf extract, comprising the steps of:

[0050] (1) Using dried Bauhinia leaves as raw materials, crushed and extracted with 95% ethanol at 65°C, 3 times for 3 hours each time;

[0051] (2) concentrating the extract under reduced pressure and reclaiming the solvent to obtain the total extract of the extract;

[0052] (3) After the total extract of the extract is dissolved in water, it is sequentially extracted with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers;

[0053] (4) The ethyl acetate layer was concentrated under reduced pressure, the obtained extract was dissolved in water, applied to NKA-9 macroporous adsorption resin, and gradiently eluted with different concentrations of ethanol to obtain the corresponding eluted fraction;

[0054] (5) The 30% ethanol eluted part was concentrated under reduced pressure, dissolved in methanol, passed through a Sephadex LH-20...

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Abstract

The invention discloses a method for preparing kaempferol and afzelin from a Chinese redbud leaf extract, and belongs to the technical field of medicines.The method comprises the following steps that dried Chinese redbud leaves serve as raw materials, are smashed and then extracted with 85% ethyl alcohol at the temperature of 50 DEG C, an extracting solution is subjected to vacuum concentration, a solvent is recycled, and total extract of the extract is obtained; adding water to dissolve the total extract of the extract, and sequentially extracting with petroleum ether, dichloromethane and ethyl acetate to obtain different organic layers; carrying out vacuum concentration on the ethyl acetate layer, adding water into the obtained extract for dissolving, loading on NKA-9 macroporous adsorption resin, and carrying out gradient elution by using ethanol with different concentrations to obtain a corresponding elution part; and concentrating the 30% ethanol elution part under reduced pressure, dissolving with methanol, passing through a Sephadex LH-20 gel chromatographic column, and eluting with methanol to obtain pure products of kaempferol and afzelin. The extraction process is simple, pure kaempferol and afzelin can be efficiently obtained, the used separation materials and solvents can be recycled, and the method is green, environmentally friendly, large in extraction amount and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for preparing kaempferol and afudouside from extracts of bauhinia leaves. Background technique [0002] Cercis chinensis is a plant of the genus Cercis in the family Leguminosae. Bauhinia bark can be used as medicine. It has the effects of clearing heat and detoxifying, promoting blood circulation and promoting qi, reducing swelling and relieving pain. , bleeding, sores and rheumatic pain. Bauhinia fruit has the effects of relieving cough and asthma, promoting qi and relieving pain, and is often used for cough with phlegm, asthma and heartache. Bauhinia root has the effect of breaking blood stasis, activating blood circulation, eliminating carbuncle and detoxifying. It is often used for irregular menstruation, stagnant abdominal pain, carbuncle sores, mumps and rabies bites. [0003] The natural compounds kaempferol and afudouside involved in the present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08
CPCC07D311/30C07D311/40C07H17/07C07H1/08
Inventor 张彭湃娄月月徐婷张林舒朋华张玉焕
Owner HENAN UNIVERSITY