Application of monensin sodium in prevention and treatment of agricultural fungal diseases
A monensin sodium, fungal disease technology, applied in application, animal husbandry, fungicides, etc., can solve the problem of ineffective gram-negative bacteria
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[0008] Embodiment 1: Indoor bacteriostatic activity assay of monensin sodium to fungus
[0009] The phytopathogenic fungi used in this experiment were strains stored at 4°C in the laboratory. The mycelial growth rate method was used. The medium used for all phytopathogenic fungi in the laboratory was potato agar dextrose medium (PDA for short). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL.
[0010] PDA medium preparation method: wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil until it can be punctured by a glass rod, filter with four layers of gauze, add distilled water in a beaker To 1000 mL, add 15-20 g of agar according to the needs of the experiment, add 20 g of glucose, stir to dissolve it fully, then divide it into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool down for later use.
[0011] experimental method
[0012] 1) Mycelia growth rate method.
[0013] 1. First cultiva...
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