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Strain Serratia sp.X10 for reducing cadmium accumulation in rice and application of strain Serratia sp.X10

A technology of bacterial strains and rice, applied in the field of agricultural microorganisms, to achieve fast growth, reduce the accumulation of heavy metal cadmium in rice, and add simple and convenient effects

Active Publication Date: 2022-03-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]Since Serratia has plant selectivity, there is no report on Serratia that can reduce cadmium accumulation in rice

Method used

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  • Strain Serratia sp.X10 for reducing cadmium accumulation in rice and application of strain Serratia sp.X10
  • Strain Serratia sp.X10 for reducing cadmium accumulation in rice and application of strain Serratia sp.X10
  • Strain Serratia sp.X10 for reducing cadmium accumulation in rice and application of strain Serratia sp.X10

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Obtaining of Serratia sp.X10:

[0049] The applicant isolated and screened a cadmium-contaminated soil (the total cadmium content in the soil sample was 0.5 mg / kg) in Shaoguan City, Guangdong Province, China, and screened a plant that could effectively passivate the heavy metal cadmium in the soil and reduce the absorption and accumulation of cadmium by rice. The strain, identified by 16sRNA gene, has 99.86% homology with Serratia nematodiphila DSM21420 (JPUX01000001), so it is Serratia nematodiphila. The strain has been sent to the China Collection of Type Cultures (CCTCC) for preservation on June 21, 2021, and its preservation number is CCTCC NO: M2021738, and the classification name is: Serratia sp. X10, address: Wuhan University, Wuhan, China. In the present invention, Serratia sp. X10 or simply referred to as strain X10.

[0050] The specific characteristics of this strain are as follows:

[0051] A. Morphological features

[0052] The cell morphology of strain ...

Embodiment 2

[0060] Soil colonization curve of strain Serratia sp.X10

[0061] The specific steps are as follows:

[0062] (1) Inoculate the fresh bacterial liquid Serratia sp.X10 cultured overnight with 1% inoculum into LB medium, and cultivate in a constant temperature shaker at 28°C and 150rpm / min for 12 hours.

[0063] (2) After centrifuging at 4000rpm / min for 10min to collect the cells, suspend the cells with ultrapure water, and suspend the cells at 10 8 CFU / g is added to the soil as a standard, and the day of addition is regarded as the 0th day.

[0064] (3) Take 5 g of rhizosphere soil at 1, 3, 5, 7, 9, 12, and 15 days, and count the colonies on antibiotic plates.

[0065] (4) Every 15 days, the same step (1) is generally supplemented with microbial inoculum.

[0066] The result is as figure 2 As shown: Serratia sp.X10 can survive in large quantities in paddy soil for about 11 days (as shown in the table below). After 11 days, it is difficult to detect due to the decrease in t...

Embodiment 3

[0070] Determination of Cadmium Content in Various Parts of Rice

[0071] The specific steps are as follows:

[0072] (1) Two treatment groups were set up in the rice pot experiment (control group without bacteria, X10 group with Serratia sp. X10 bacteria), five parallel groups in each group.

[0073] Cadmium balance was performed on the soil before planting: 5 mg / kg of cadmium was added to each pot, and the soil was immersed with excess water and then balanced for one week.

[0074] (2) After the soil balance is over, transplant 3 rice seedlings with the same growth in each pot, resuspend the Serratiasp. X10 strain with physiological saline, and apply it to the five pots of soil in the X10 group on average. Each pot of Serratiasp. The final concentration of X10 bacteria is 10 8 CFU / g (dry soil), in the control group, an equal amount of normal saline was added to each pot.

[0075] (3) Two treatment groups were set up in the rice field experiment (control group without bact...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and discloses a strain Serratia sp.X10 for reducing cadmium accumulation in rice and application of the strain Serratia sp.X10, and the preservation number of the strain Serratia sp.X10 is CCTCC (China Center for Type Culture Collection) NO: M2021738. The strain is directly sprayed or irrigated in soil. The X10 can reduce the absorption and accumulation of the rice on the cadmium in the soil through the adsorption of the strain on the cadmium and the change of the bioavailability of the cadmium in the soil, and meanwhile, the inhibition effect of the heavy metal cadmium on the growth of the rice is relieved. The strain Serratia sp.X10 can efficiently passivate cadmium in soil, so that cadmium accumulation of rice is reduced, and the diversity of original microorganisms in the soil is not changed. The method is easy to operate, economical, efficient, remarkable in effect and capable of reducing harm of cadmium-polluted soil to rice and has high application value and potential.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a strain Serratiasp.X10 for reducing cadmium accumulation in rice and its application. Background technique [0002] Cadmium (Cd) is a silver and ductile toxic metal, easily soluble in acid but insoluble in alkali, and its valences are Cd(II) and Cd(I). The forms of cadmium in soil can be divided into four forms: acid extractable state, reducible state, oxidizable state and residue state. Acid-extractable and reducible cadmium are the most easily utilized by plants among the four forms. Oxidable and residual cadmium have the highest stability and are not easily absorbed and utilized by plants, and various forms of cadmium can be transformed into each other. Cadmium in soil is highly mobile and easily accumulated by plants into the food chain, and then accumulates in the human body and produces irreversible toxic effects. In addition, cadmium in the air can als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/20A01P21/00C12R1/425C09K101/00
CPCC09K17/14A01N63/20C09K2101/00
Inventor 李明顺王孟杰范蓉荣王革娇
Owner HUAZHONG AGRI UNIV
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