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Green separation and purification method and application of blueberry polysaccharide

A technology for separation and purification of polysaccharides, applied in the field of green separation and purification of blueberry polysaccharides, which can solve the problems of blueberry polysaccharides with many impurities, organic residues, and complicated separation and purification methods, and achieve high purity, improved product quality, and simple and uncomplicated methods Effect

Pending Publication Date: 2022-03-11
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the blueberry polysaccharide extracted by the prior art has many impurities, and a large amount of organic matter will be added in the separation and purification process. The separation and purification method is relatively complicated. quality

Method used

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  • Green separation and purification method and application of blueberry polysaccharide
  • Green separation and purification method and application of blueberry polysaccharide
  • Green separation and purification method and application of blueberry polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0090] A green separation and purification method of blueberry polysaccharides, comprising the following steps:

[0091] (1) Sample separation: The crude extract of blueberry anthocyanins is applied to the column by wet method through macroporous resin, let stand for adsorption, and then eluted with distilled water, and the polysaccharide is detected by the phenol-sulfuric acid method until the elution solution is sugar-free. Split out, and then concentrate the blueberry polysaccharide eluate to obtain blueberry polysaccharide extract; the quality of the blueberry anthocyanin crude extract and resin is applied to the column according to the ratio of 1:30 wet method, and the elution time is 60min; the concentration temperature is 63°C;

[0092] (2) Alcohol precipitation: dissolve the blueberry polysaccharide extract of step (1) with hot distilled water, then add ethanol under stirring, and after standing overnight, filter and collect the precipitate; the temperature of the dist...

Embodiment 2

[0097] A green separation and purification method of blueberry polysaccharides, comprising the following steps:

[0098] (1) Sample separation: The crude extract of blueberry anthocyanins is applied to the column by wet method through macroporous resin, let stand for adsorption, and then eluted with distilled water, and the polysaccharide is detected by the phenol-sulfuric acid method until the elution solution is sugar-free. Split out, and then concentrate the blueberry polysaccharide eluate to obtain blueberry polysaccharide extract; the quality of the blueberry anthocyanin crude extract and resin is applied to the column according to the ratio of 1:30 wet method, and the elution time is 65min; the concentration temperature is 68°C;

[0099] (2) Alcohol precipitation: dissolve the blueberry polysaccharide extract of step (1) with hot distilled water, then add ethanol under stirring, and after standing overnight, filter and collect the precipitate; the temperature of the dist...

Embodiment 3

[0104] A green separation and purification method of blueberry polysaccharides, comprising the following steps:

[0105] (1) Sample separation: The crude extract of blueberry anthocyanins is applied to the column by wet method through macroporous resin, let stand for adsorption, and then eluted with distilled water, and the polysaccharide is detected by the phenol-sulfuric acid method until the elution solution is sugar-free. Separate the flow, and then concentrate the blueberry polysaccharide eluate to obtain blueberry polysaccharide extract; the quality of the blueberry anthocyanin crude extract and resin is applied to the column according to the ratio of 1:30 wet method, and the elution time is 61min; the concentration temperature is 64°C;

[0106] (2) Alcohol precipitation: dissolve the blueberry polysaccharide extract of step (1) with hot distilled water, then add ethanol under stirring, and after standing overnight, filter and collect the precipitate; the temperature of ...

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Abstract

The invention discloses a green separation and purification method of blueberry polysaccharide, which comprises the following steps: carrying out wet column loading on a blueberry anthocyanin crude extract through macroporous resin, standing for adsorption, eluting with distilled water, detecting polysaccharide, concentrating, dissolving with hot distilled water, adding ethanol while stirring, standing overnight, filtering, collecting precipitate, dissolving into a polysaccharide solution, and drying to obtain the blueberry polysaccharide. Performing decoloration, centrifugal filtration, deproteinization, centrifugal filtration, supernate taking and wet loading on macroporous resin, performing gradient elution by using four solutions with different concentrations through column chromatography, respectively collecting each fraction, respectively dialyzing by using distilled water, respectively performing vacuum concentration and vacuum freeze drying on the liquid in a dialysis bag, and thus obtaining the compound. The method comprises the following steps of: separating and purifying blueberry polysaccharides Fr-A1, Fr-A2, Fr-A3 and Fr-A4 to obtain four separated and purified blueberry polysaccharides Fr-A1, Fr-A2, Fr-A3 The blueberry polysaccharide obtained after separation and purification through the method is high in purity, the method is simple and not complex, the product obtained after separation and purification is free of organic matter residues, and the product quality is improved.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and in particular relates to a green separation and purification method and application of blueberry polysaccharides. Background technique [0002] Blueberry (Vaccini μm μliginos μm), also known as bilberry or blueberry, belongs to Ericaceae (Ericaceae) Vaccinium genus (Vaccini μ mspp.) deciduous shrub plant. Known as "the king of berries in the world", the fruit is rich in anthocyanins, polysaccharides, vitamins, dietary fiber, protein, fat, VA, VE, SOD and other nutrients, among which the anthocyanins content in blueberries Higher, anthocyanins and polysaccharides are the main functional substances of blueberries, and anthocyanins have been widely studied because of their unique physiological activities. The active substances contained in blueberry fruit have anti-aging, relieve visual fatigue, improve memory, anti-oxidation, anti-cancer and other effects. [...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L33/125A61K31/715A61P39/06
CPCC08B37/0003A23L33/125A61K31/715A61P39/06A23V2002/00A23V2250/51
Inventor 李冰晶罗丽平王代波赵景芳刘国华封天洪
Owner GUIZHOU INST OF BIOLOGY
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