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Peripheral blood mobilization concentrated cell therapeutic agent for treating premature ovarian failure

A peripheral blood and cell technology, applied in the fields of biotechnology and biomedicine, can solve the problems of long time, complicated operation, poor repeatability of results, etc., and achieve fast and accurate data tracking and documentation, and the effect of a wide range of clinical application scenarios.

Pending Publication Date: 2022-03-18
深圳博雅感知医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, mobilized peripheral blood is traditionally separated by density gradient centrifugation manually, which has very difficult problems such as complicated operation, long time-consuming, easy contamination, and poor reproducibility of results.

Method used

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  • Peripheral blood mobilization concentrated cell therapeutic agent for treating premature ovarian failure
  • Peripheral blood mobilization concentrated cell therapeutic agent for treating premature ovarian failure
  • Peripheral blood mobilization concentrated cell therapeutic agent for treating premature ovarian failure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1: Rapid separation and preparation of mobilized peripheral blood concentrated cells

[0102] (1) Provide biological samples to mobilize peripheral blood (sample volume of 20-200ml can be processed), put it in a sterile bag containing anticoagulant for standby, and take another 1ml sample for testing;

[0103] (2) Remove the protective cap on the input tube of the automatic cell separation system, connect the syringe to the Luer lock connector of the input tube, pass through the thrombus filter at a slow and steady speed, and transfer the anticoagulated biological sample to a disposable In the sterile separation cup, shake the mixed sample along the horizontal axis; the automatic cell separation system is a closed PXP separation system, which consists of four parts: a) disposable sterile separation cup, b) control module, c) Separate base for data transmission, d) DataTrak software processing system;

[0104] (3) Put the disposable separation cup into the con...

Embodiment 1a： reference Embodiment 1 preparation ，，5 to 5。 Embodiment 1b： reference Embodiment 1 preparation ，，5 to 5。 Embodiment 1c

[0117]Example 1a: Refer to Example 1 to prepare mobilized peripheral blood concentrated cells, the difference is that no histidine is added to the anticoagulant, and 5 biological samples are processed to obtain 5 mobilized peripheral blood concentrated cells. Example 1b: Refer to Example 1 to prepare mobilized peripheral blood concentrated cells, the difference is that phosphatidylcholine is not added to the anticoagulant, and 5 biological samples are processed to obtain 5 mobilized peripheral blood concentrated cells. Example 1c: Refer to Example 1 to prepare mobilized peripheral blood concentrated cells, the difference is that neither histidine nor phosphatidylcholine is added to the anticoagulant, and 5 biological samples are processed to obtain 5 mobilized peripheral blood Concentrate cells. The blood plasma layer obtained in the operation of embodiment 1, embodiment 1a, embodiment 1b, embodiment 1c is diluted 1 times with double distilled water, measures absorbance at 540...

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Abstract

The invention relates to a peripheral blood mobilizing concentrated cell therapeutic agent for treating premature ovarian failure. The method for preparing peripheral blood, such as mobilized peripheral blood concentrated cells, comprises the following steps: providing peripheral blood, such as mobilized peripheral blood concentrated cells, of a biological sample, and placing the peripheral blood in a sterile bag containing an anticoagulant for later use; a protective cap on an input tube of the automatic cell separation system is taken down, an injector is connected to a Luer locking connector of the input tube and passes through a thrombus filter at a slow and stable speed, an anticoagulant biological sample is transferred into a disposable sterile separation cup, and the mixed sample is shaken along a horizontal shaft; the automatic cell separation system is a closed PXP separation system and consists of four parts, namely a disposable sterile separation cup, a control module, a separation base and a software processing system, and putting the disposable separation cup into a control module, and centrifuging in a programmable centrifugal machine to obtain mobilized peripheral blood concentrated cells. The method disclosed by the invention has excellent effects as described in the specification.

Description

technical field [0001] The invention belongs to the fields of biotechnology and biomedicine, and relates to a method for treating premature ovarian failure (Premature ovarian failure, POF) by using a cell therapy agent. Specifically, the present invention relates to the rapid separation and preparation of a cell therapy agent for mobilizing peripheral blood concentrated cells from mobilized peripheral blood (Mobilized Peripheral Blood, MPB), and then using the mobilized peripheral blood concentrated cells to treat premature ovarian insufficiency and ovarian approach to premature aging. Using the method of the present invention can effectively improve the efficiency of separating mobilized peripheral blood concentrated cells from mobilized peripheral blood, and provide a safe, efficient and simple preparation of mobilized peripheral blood concentrated cells for the treatment of premature ovarian failure and ovarian insufficiency such as premature ovarian failure Methods of Ova...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/0775C12N5/0789A61K35/14A61P15/08
CPCC12N5/0647C12N5/0665C12N5/0692A61K35/14A61P15/08C12N2509/10
Inventor 肖海蓉李容徐勇
Owner 深圳博雅感知医疗科技有限公司
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