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Method for rapid fluorescence detection of TNT

A fluorescence detection and fast technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve the problems of cumbersome synthesis steps and processing, and achieve low-cost and environment-friendly effects

Pending Publication Date: 2022-03-25
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0006] However, these probe molecules require tedious synthesis steps and post-processing processes

Method used

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  • Method for rapid fluorescence detection of TNT
  • Method for rapid fluorescence detection of TNT
  • Method for rapid fluorescence detection of TNT

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of detection reagents:

[0027] At room temperature, measure 0.5mL of 50mM Cu(NO 3 ) 2 Aqueous solution and 2.5mL 50mM glutathione aqueous solution, stir and mix for 5 minutes, add 2mL water to continue to disperse evenly, adjust the pH to 5.0 with NaOH with a molar concentration of 2M to a light yellow transparent solution, and then add a total solvent volume fraction of 95% The ethanol aggregates to obtain a fluorescent detection reagent;

[0028] Fluorescent detection of 2,4,6-trinitrotoluene:

[0029] Measure 3mL of the detection reagent in a quartz cuvette, then add 1mg of 2,4,6-trinitrotoluene (TNT) solid particles, scan the fluorescence emission spectrum of the detection reagent before and after the reaction with a fluorescence spectrometer, and measure the change in fluorescence intensity The value is the ordinate, the wavelength is the abscissa, and the fluorescence emission spectrum before and after the solution reaction is obtained, such as Fig...

Embodiment 2

[0031] Preparation of detection reagents:

[0032] At room temperature, measure 0.5mL of 30mM Cu(NO 3 ) 2 Aqueous solution and 2.5mL 30mM glutathione aqueous solution, stir and mix evenly for 1min, add 2mL water to continue to disperse evenly, adjust the pH to 4.0 with NaOH with a molar concentration of 0.5M, and then add absolute ethanol with a total solvent volume fraction of 98% Aggregation to obtain a fluorescent detection reagent;

[0033] Fluorescent detection of 2,4,6-trinitrotoluene:

[0034] Measure 3mL of the detection reagent into a quartz cuvette, add 1mg of 2,4,6-trinitrotoluene (TNT) methanol solution, and irradiate with a 365nm ultraviolet lamp, the fluorescence is obviously quenched, so as to determine the TNT The presence.

Embodiment 3

[0036] Preparation of detection reagents:

[0037] At room temperature, measure 0.5mL of 50mM Cu(NO 3 ) 2 Aqueous solution and 2.5mL 50mM glutathione aqueous solution, stir and mix for 8 minutes, add 1.5mL water to continue to disperse evenly, adjust the pH to 6.0 with molar concentration, 1M NaOH, and then add anhydrous Ethanol aggregates to obtain a fluorescent detection reagent;

[0038] Measure 3 mL of the detection reagent into a quartz cuvette, and then add 200 μL of 1 mM 2,4,6-trinitrotoluene (TNT) methanol solution. Irradiated with 365nm ultraviolet light, the orange fluorescence of copper clusters was obviously quenched.

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Abstract

The invention provides a method for rapid fluorescence detection of TNT (trinitrotoluene). According to the method, fluorescence detection is carried out based on a glutathione modified copper nanocluster (GSH-CuNC). The preparation method comprises the following steps: mixing a copper nitrate aqueous solution and a glutathione aqueous solution at room temperature, uniformly stirring, adjusting the pH value by using sodium hydroxide, and adding ethanol to obtain the orange fluorescence detection reagent. The detected suspicious particles or solution is directly dropwise added into the detection reagent, and if the suspicious particles contain TNT, the orange fluorescence of the detection reagent is obviously quenched. The detection reagent is efficient and rapid in TNT detection, has the advantages of being easy to operate, low in price, high in visualization degree and the like, and facilitates TNT component detection in complex environments and standard explosive raw materials. Besides, after the reagent is in contact with the TNT, naked eye visual fluorescence quenching can be achieved within 30 seconds, and complicated analysis equipment is not needed, so that low-cost and real-time detection of the TNT is realized, and time and labor are saved. And an effective technical means is provided for the detection of an explosive case.

Description

technical field [0001] The invention belongs to the field of detection of environmental and standard explosive raw materials, and provides a green and non-toxic rapid fluorescence detection method for 2,4,6-trinitrotoluene which can not only be stored stably. Background technique [0002] In recent years, carrying out research work such as explosive detection and analysis is the premise and necessary means to effectively curb explosive attacks. The nitro explosives in explosives are powerful. TNT, known as the king of explosions, is Stability and other characteristics, it is still widely used so far; at the same time, 2,4,6-trinitrotoluene (TNT) in TNT will pollute the environment, have serious toxic effects on plants, and cause irreversible harm to the human body. Therefore, the detection of 2,4,6-trinitrotoluene (TNT) is of great significance to the safety of people's lives and property. (Journal of Materials Chemistry C, 2018, 6(45): 12142-58) [0003] Currently, common...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N2021/6432
Inventor 窦新存代卓华王广发
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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