Preparation method of cationic starch-based glutathione peroxidase

A technology of glutathione peroxide and cationic starch, applied in the field of starch modification, can solve the problems of restricting industrialization development, high cost, limited and other problems, so as to improve the substrate binding ability, improve the catalytic activity, and expand the scope of application Effect

Active Publication Date: 2022-04-01
BEIBU GULF UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Usually, it is difficult for most small molecule bionic GPx to realize the overall simulation of GPx synergistic catalytic process
Supramolecular biomimetic GPx can usually realize the synergistic catalysis of various catalytic primitives, but it can only be realized under specific conditions, which has certain limitations in application.
The structure and catalytic activity of biomimetic GPx with protein backbone can be compared with natural GPx, but the limited raw materials and high cost limit its industrialization development

Method used

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  • Preparation method of cationic starch-based glutathione peroxidase
  • Preparation method of cationic starch-based glutathione peroxidase
  • Preparation method of cationic starch-based glutathione peroxidase

Examples

Experimental program
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Embodiment 1

[0029] A kind of preparation method of cationic starch-based glutathione peroxidase (preparation route sees figure 1 ), including the following steps:

[0030] (1) 30g of cassava starch (Cassava starch) was added into the mixed solvent of absolute ethanol / water and stirred to prepare starch emulsion. Octenyl succinic anhydride (3.5% of starch dry weight) was dissolved in 5ml of ethanol, and it was slowly added dropwise into the flask of starch emulsion. During the dropwise reaction, the pH of the reaction system was maintained at about 8.5. React at 40°C for 6 hours. After the reaction, deionized water, 75% ethanol and absolute ethanol are used to wash, filter, and dry to obtain OSAstarch.

[0031] (2) 123.90 mg of selenium and 178.10 mg of sodium borohydride were added to 20 mL of deionized water, and a stock solution of sodium selenium hydride (NaSeH) was prepared under a nitrogen atmosphere. 5.0g of OSA starch was added to 80mL of absolute ethanol (the volume ratio of ab...

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Abstract

The invention relates to the technical field of starch modification, in particular to a preparation method of cationic starch-based glutathione peroxidase. The invention relates to a preparation method of cationic starch-based glutathione peroxidase. The preparation method comprises the following steps: (1) preparing OSA (Octenyl Succinic Acid) starch ester; (2) preparation of selenium starch (Se-Starch); and (3) preparation of the cationic Se-Starch (Cation Se-Starch). The cationic selenized starch can simulate a catalytic center, a hydrophobic microenvironment and a substrate recognition site of natural glutathione peroxidase (GPx), and meanwhile, the effective matching and synergistic effect of three catalytic elements of the catalytic center, the hydrophobic microenvironment and the recognition site plays a positive role in improving the antioxidant catalytic activity of the cationic selenized starch.

Description

technical field [0001] The invention relates to the technical field of starch modification, in particular to a preparation method of cationic starch-based glutathione peroxidase. Background technique [0002] Glutathione peroxidase (GPx) is an important antioxidant enzyme that can scavenge reactive oxygen species and protect cells from oxidative damage. However, due to the limited source of natural GPx and its poor stability, its application development is limited, so the design and synthesis of highly efficient GPx antioxidant enzyme mimics has attracted widespread attention in the academic community. Based on the understanding of the structure and characteristics of GPx, studies have shown that catalytic centers, hydrophobic microenvironments and recognition sites are important catalytic units for the construction of efficient biomimetic GPx, and their synergistic catalysis plays a very important role in improving the activity of biomimetic GPx . [0003] The skeleton-ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B31/16C08B31/04C08B31/12C12N9/08
Inventor 张瑞瑞石成郑韵英尹艳镇梁兴唐张艳军
Owner BEIBU GULF UNIV
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