Typing model, typing method and kit for diffuse large B-cell lymphoma

A lymphoma and B cell technology, applied in the field of biological detection, can solve the problems of accelerated disease process and poor prognosis of patients, and achieves the effect of wide coverage, easy clinical tracking, and easy clinical promotion.

Pending Publication Date: 2022-04-05
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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Problems solved by technology

These will accelerate the progression of the disease, and patients with such mutations often have a poor prognosis

Method used

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  • Typing model, typing method and kit for diffuse large B-cell lymphoma
  • Typing model, typing method and kit for diffuse large B-cell lymphoma
  • Typing model, typing method and kit for diffuse large B-cell lymphoma

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Embodiment 1

[0138] The kit adopts probe capture technology. Firstly, human genomic nucleic acid (DNA) is used as material to prepare a pre-library through fragmentation, end repair, adapter addition, and PCR amplification; then, biotin-labeled oligonucleotides are used to The acid probe is hybridized with the pre-library; then the streptavidin magnetic beads are combined with the probe to capture the target region; finally, the final capture library is obtained through PCR enrichment. The capture library was sequenced using a gene sequencer (model: MGISEQ-2000, MGISEQ-200, produced by MGI Corporation) for high-throughput sequencing.

[0139] For sequencing data, use bioinformatics software (Fastp-v0.21.0, FastQC, bwa-v0.7.17, samtools-1.11, GATK3.8, lianti-v1.0, VarScan-v2.3) to interpret DLBCL-related mutation information .

[0140] The composition of the kit is shown in Table 4:

[0141] Table 4

[0142]

[0143]

[0144] Primer sequences are shown in Table 5:

[0145] table 5...

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Abstract

The invention relates to the field of biological detection, in particular to a typing method and a typing model for diffuse large B-cell lymphoma, and further relates to a targeted sequencing kit and a typing kit for the diffuse large B-cell lymphoma. The method comprises the following steps: acquiring a diffuse large B-cell lymphoma sample with the lowest sample size; mutation information of 18 related genes in the diffuse large B-cell lymphoma sample is detected; and the mutation information is clustered by adopting a PAM algorithm, a typing model is established, and the typing model containing an MCD type, an N1 type, an EZB type, a BN2 type and an Others type is obtained. According to comparison of consistency, a result separated by applying the NEJM50 gene model is highly consistent with a result of the model disclosed by the invention, typing can be completed only by detecting mutation information of 18 genes, the sequencing cost is relatively low, clinical popularization is facilitated, the detection cost is reduced, and the method is economical and practical.

Description

technical field [0001] The present invention relates to the field of biological detection, in particular to a typing model and typing method for diffuse large B-cell lymphoma, and also relates to a targeted sequencing kit and typing kit for diffuse large B-cell lymphoma . Background technique [0002] Lymphoma is the hematological malignancy with the highest incidence rate in my country, and ranks among the top ten cancer incidence rates in my country. It can be roughly divided into Hodgkin's Lymphoma (HL) and non-Hodgkin's Lymphoma ( Non-Hodgkin's Lymphoma, NHL) two categories. Diffuse Large B Cell Lymphoma (DLBCL) is a relatively common and highly heterogeneous aggressive lymphoma among non-Hodgkin's lymphomas. In my country, the incidence rate accounts for 30% of the total number of non-Hodgkin's lymphoma (NHL) and more than 50% of the total number of B-cell non-Hodgkin's lymphoma (B-NHL). In terms of prognosis and drug selection, the International Prognostic Index (IPI...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11G16B20/50G16B20/20G16B25/20
Inventor 赵维莅许彭鹏付迪熊慧俞浩赵天雨
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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