Typing model, typing method and kit for diffuse large B-cell lymphoma
A lymphoma and B cell technology, applied in the field of biological detection, can solve the problems of accelerated disease process and poor prognosis of patients, and achieves the effect of wide coverage, easy clinical tracking, and easy clinical promotion.
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[0138] The kit adopts probe capture technology. Firstly, human genomic nucleic acid (DNA) is used as material to prepare a pre-library through fragmentation, end repair, adapter addition, and PCR amplification; then, biotin-labeled oligonucleotides are used to The acid probe is hybridized with the pre-library; then the streptavidin magnetic beads are combined with the probe to capture the target region; finally, the final capture library is obtained through PCR enrichment. The capture library was sequenced using a gene sequencer (model: MGISEQ-2000, MGISEQ-200, produced by MGI Corporation) for high-throughput sequencing.
[0139] For sequencing data, use bioinformatics software (Fastp-v0.21.0, FastQC, bwa-v0.7.17, samtools-1.11, GATK3.8, lianti-v1.0, VarScan-v2.3) to interpret DLBCL-related mutation information .
[0140] The composition of the kit is shown in Table 4:
[0141] Table 4
[0142]
[0143]
[0144] Primer sequences are shown in Table 5:
[0145] table 5...
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