Lung cancer organoid culture medium, culture reagent combination and culture method thereof

Abstract

This application relates to the technical field of biological tissue engineering, and discloses a lung cancer organoid culture medium and its culture reagent combination and culture method. The lung cancer organoid culture medium includes complete medium and self-derived conditioned medium; complete medium includes conditioned culture Base, basal medium, compound antibiotics and growth factors; self-sourced conditioned medium is obtained by culturing fibroblasts extracted from lung cancer tissue using tumor-associated fibroblast medium. The synergy between the components of the culture medium accelerates the formation and growth of lung cancer organoid cells, increases the number of harvested cells and improves the culture speed; moreover, the organoids cultured in vitro are more bionic and can be applied to the personalized culture of lung cancer organoids , able to maintain the physical structure and pathological characteristics of each patient's primary tissue.

Description

Lung cancer organoid culture medium and its combination of culture reagents and culture method technical field The application relates to biological tissue engineering technical field, for example relates to a kind of lung cancer organoid culture fluid and culture test thereof. Agent combinations and culture methods. Background technique Lung cancer is one of the top ten malignant tumors in my country. At present, the etiology of lung cancer is not fully understood, and it is currently believed that the main It is the result of the combined effect of environmental factors and genetic factors. Lung cancer organoids are of great significance for research. Although there are currently Some studies on lung cancer organoid culture medium have reported, but the culture process is slow to grow, increasing the cost of time investment; Low. In the process of realizing the embodiment of the present application, it is found that there are at least the following problems in...

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Problems solved by technology

Moreover, the amount of cells obtained is also very small. In order to harvest more cells and conduct downstream experiments, it takes longer, which makes the construction process of the entire organ slow, resulting in high time costs for lung cancer organoid research. In addition, more The most important thing is that after a period of time in vitro culture of organoids, EMT transition will occur, and the mesenchymal properties in vivo will be gradually lost, which will be quite different from the real tumor properties in vivo

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