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Application of 2-bromopalmitic acid in preparation of medicine for treating spermatogenic dysfunction related diseases

A dysfunctional, bromopalmitic acid technology, applied in the field of biomedicine, to restore the integrity of the blood-testis barrier and improve spermatogenic dysfunction

Active Publication Date: 2022-04-12
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of 2-bromopalmitic acid to improve spermatogenic function

Method used

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  • Application of 2-bromopalmitic acid in preparation of medicine for treating spermatogenic dysfunction related diseases
  • Application of 2-bromopalmitic acid in preparation of medicine for treating spermatogenic dysfunction related diseases
  • Application of 2-bromopalmitic acid in preparation of medicine for treating spermatogenic dysfunction related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The example of 2-bromopalmitic acid improving the barrier damage of Sertoli cells stimulated by saturated fatty acids in vitro is as follows.

[0031] (1) Isolation and culture of mouse primary Sertoli cells:

[0032] Take 20-day-old ICR male mice, kill them by neck dislocation, soak them in 75% disinfectant alcohol for 2-5 minutes, incise the skin of the lower abdomen in an ultra-clean bench, take out the testes, cut off the seminal vesicles from the tail of the epididymis, take both testes, and put them in In a culture dish of PBS at 37°C, use ophthalmic tweezers to tear off adipose tissue, testicular tunica and blood vessels, expose the seminiferous tubules, and put them into another culture dish with PBS. Cut tissue to less than 1mm 3 Put them in 5ml d0.25% (m / V) trypsin, shake and digest at 37°C for 4-6 minutes to disperse the seminiferous tubules. Wash once with 25-30ml of PBS, remove the supernatant. Digest with 5ml 0.1% (m / V) type 1 collagenase at 37°C for 6-...

Embodiment 2

[0047] 2-Bromopalmitic acid can significantly improve spermatogenic dysfunction and blood-testis barrier damage caused by lipid metabolism disorders in vivo. Examples are as follows.

[0048] (1) Selection of experimental materials:

[0049] 4-week-old ICR male mice were selected as animals; 2-BP and PA were selected from Sigma Company of the United States. 2-BP was prepared as a 100 mg / ml storage solution with ethanol, and was injected with physiological saline as a 5 mg / ml working solution during the experiment. PA was chelated with BSA and prepared into a 20 mM (about 5.13 mg / ml) storage solution (ie, working solution) with physiological saline as a solvent.

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Abstract

The invention discloses an application of 2-bromopalmitic acid in preparation of a medicine for treating spermatogenic dysfunction related diseases. According to the invention, it is determined for the first time that 2-bromopalmitic acid can improve barrier damage of testicular sertoli cells under the stimulation of saturated fatty acid; by constructing a highly saturated fatty acid mouse spermatogenesis dysfunction model, it is clear that 2-bromopalmitic acid can effectively improve spermatogenesis dysfunction caused by highly saturated fatty acid in vivo and recover the barrier function of testicular sertoli cells; the invention also provides an in-vivo experimental data basis for treating spermatogenic dysfunction caused by lipid metabolism disorder by using 2-bromopalmitic acid, and provides a new method for treating the disease.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of 2-bromopalmitic acid in the preparation of medicines for treating diseases related to spermatogenic dysfunction. Background technique [0002] Infertility is an important medical problem that cannot be ignored in modern society. Male factors account for 30-50% of infertile couples. Sperm dysfunction is one of the main causes of male infertility. In recent years, the rising rate of obesity has made lipid metabolism disorder an important cause of spermatogenic dysfunction. The abnormal increase of saturated fatty acid is a major feature of lipid metabolism disorder, and it is also a key factor causing spermatogenesis dysfunction. Therefore, the treatment of spermatogenic dysfunction caused by lipid metabolism disorder and abnormal increase of saturated fatty acid has become an urgent medical problem to be solved in recent years. [0003] The pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/20A61P15/08
CPCY02A50/30
Inventor 戈榭姚兵马汝钧靖俊赵薇
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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